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• Null and Alternative Hypotheses | Definitions & Examples

Null & Alternative Hypotheses | Definitions, Templates & Examples

Published on May 6, 2022 by Shaun Turney . Revised on June 22, 2023.

The null and alternative hypotheses are two competing claims that researchers weigh evidence for and against using a statistical test :

• Null hypothesis ( H 0 ): There’s no effect in the population .
• Alternative hypothesis ( H a or H 1 ) : There’s an effect in the population.

Table of contents

Answering your research question with hypotheses, what is a null hypothesis, what is an alternative hypothesis, similarities and differences between null and alternative hypotheses, how to write null and alternative hypotheses, other interesting articles, frequently asked questions.

The null and alternative hypotheses offer competing answers to your research question . When the research question asks “Does the independent variable affect the dependent variable?”:

• The null hypothesis ( H 0 ) answers “No, there’s no effect in the population.”
• The alternative hypothesis ( H a ) answers “Yes, there is an effect in the population.”

The null and alternative are always claims about the population. That’s because the goal of hypothesis testing is to make inferences about a population based on a sample . Often, we infer whether there’s an effect in the population by looking at differences between groups or relationships between variables in the sample. It’s critical for your research to write strong hypotheses .

You can use a statistical test to decide whether the evidence favors the null or alternative hypothesis. Each type of statistical test comes with a specific way of phrasing the null and alternative hypothesis. However, the hypotheses can also be phrased in a general way that applies to any test.

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The null hypothesis is the claim that there’s no effect in the population.

If the sample provides enough evidence against the claim that there’s no effect in the population ( p ≤ α), then we can reject the null hypothesis . Otherwise, we fail to reject the null hypothesis.

Although “fail to reject” may sound awkward, it’s the only wording that statisticians accept . Be careful not to say you “prove” or “accept” the null hypothesis.

Null hypotheses often include phrases such as “no effect,” “no difference,” or “no relationship.” When written in mathematical terms, they always include an equality (usually =, but sometimes ≥ or ≤).

You can never know with complete certainty whether there is an effect in the population. Some percentage of the time, your inference about the population will be incorrect. When you incorrectly reject the null hypothesis, it’s called a type I error . When you incorrectly fail to reject it, it’s a type II error.

Examples of null hypotheses

The table below gives examples of research questions and null hypotheses. There’s always more than one way to answer a research question, but these null hypotheses can help you get started.

*Note that some researchers prefer to always write the null hypothesis in terms of “no effect” and “=”. It would be fine to say that daily meditation has no effect on the incidence of depression and p 1 = p 2 .

The alternative hypothesis ( H a ) is the other answer to your research question . It claims that there’s an effect in the population.

Often, your alternative hypothesis is the same as your research hypothesis. In other words, it’s the claim that you expect or hope will be true.

The alternative hypothesis is the complement to the null hypothesis. Null and alternative hypotheses are exhaustive, meaning that together they cover every possible outcome. They are also mutually exclusive, meaning that only one can be true at a time.

Alternative hypotheses often include phrases such as “an effect,” “a difference,” or “a relationship.” When alternative hypotheses are written in mathematical terms, they always include an inequality (usually ≠, but sometimes < or >). As with null hypotheses, there are many acceptable ways to phrase an alternative hypothesis.

Examples of alternative hypotheses

The table below gives examples of research questions and alternative hypotheses to help you get started with formulating your own.

Null and alternative hypotheses are similar in some ways:

• They’re both answers to the research question.
• They both make claims about the population.
• They’re both evaluated by statistical tests.

However, there are important differences between the two types of hypotheses, summarized in the following table.

To help you write your hypotheses, you can use the template sentences below. If you know which statistical test you’re going to use, you can use the test-specific template sentences. Otherwise, you can use the general template sentences.

General template sentences

The only thing you need to know to use these general template sentences are your dependent and independent variables. To write your research question, null hypothesis, and alternative hypothesis, fill in the following sentences with your variables:

Does independent variable affect dependent variable ?

• Null hypothesis ( H 0 ): Independent variable does not affect dependent variable.
• Alternative hypothesis ( H a ): Independent variable affects dependent variable.

Test-specific template sentences

Once you know the statistical test you’ll be using, you can write your hypotheses in a more precise and mathematical way specific to the test you chose. The table below provides template sentences for common statistical tests.

Note: The template sentences above assume that you’re performing one-tailed tests . One-tailed tests are appropriate for most studies.

If you want to know more about statistics , methodology , or research bias , make sure to check out some of our other articles with explanations and examples.

• Normal distribution
• Descriptive statistics
• Measures of central tendency
• Correlation coefficient

Methodology

• Cluster sampling
• Stratified sampling
• Types of interviews
• Cohort study
• Thematic analysis

Research bias

• Implicit bias
• Cognitive bias
• Survivorship bias
• Availability heuristic
• Nonresponse bias
• Regression to the mean

Hypothesis testing is a formal procedure for investigating our ideas about the world using statistics. It is used by scientists to test specific predictions, called hypotheses , by calculating how likely it is that a pattern or relationship between variables could have arisen by chance.

Null and alternative hypotheses are used in statistical hypothesis testing . The null hypothesis of a test always predicts no effect or no relationship between variables, while the alternative hypothesis states your research prediction of an effect or relationship.

The null hypothesis is often abbreviated as H 0 . When the null hypothesis is written using mathematical symbols, it always includes an equality symbol (usually =, but sometimes ≥ or ≤).

The alternative hypothesis is often abbreviated as H a or H 1 . When the alternative hypothesis is written using mathematical symbols, it always includes an inequality symbol (usually ≠, but sometimes < or >).

A research hypothesis is your proposed answer to your research question. The research hypothesis usually includes an explanation (“ x affects y because …”).

A statistical hypothesis, on the other hand, is a mathematical statement about a population parameter. Statistical hypotheses always come in pairs: the null and alternative hypotheses . In a well-designed study , the statistical hypotheses correspond logically to the research hypothesis.

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• Knowledge Base
• Null and Alternative Hypotheses | Definitions & Examples

Null and Alternative Hypotheses | Definitions & Examples

Published on 5 October 2022 by Shaun Turney . Revised on 6 December 2022.

The null and alternative hypotheses are two competing claims that researchers weigh evidence for and against using a statistical test :

• Null hypothesis (H 0 ): There’s no effect in the population .
• Alternative hypothesis (H A ): There’s an effect in the population.

The effect is usually the effect of the independent variable on the dependent variable .

Table of contents

Answering your research question with hypotheses, what is a null hypothesis, what is an alternative hypothesis, differences between null and alternative hypotheses, how to write null and alternative hypotheses, frequently asked questions about null and alternative hypotheses.

The null and alternative hypotheses offer competing answers to your research question . When the research question asks “Does the independent variable affect the dependent variable?”, the null hypothesis (H 0 ) answers “No, there’s no effect in the population.” On the other hand, the alternative hypothesis (H A ) answers “Yes, there is an effect in the population.”

The null and alternative are always claims about the population. That’s because the goal of hypothesis testing is to make inferences about a population based on a sample . Often, we infer whether there’s an effect in the population by looking at differences between groups or relationships between variables in the sample.

You can use a statistical test to decide whether the evidence favors the null or alternative hypothesis. Each type of statistical test comes with a specific way of phrasing the null and alternative hypothesis. However, the hypotheses can also be phrased in a general way that applies to any test.

The null hypothesis is the claim that there’s no effect in the population.

If the sample provides enough evidence against the claim that there’s no effect in the population ( p ≤ α), then we can reject the null hypothesis . Otherwise, we fail to reject the null hypothesis.

Although “fail to reject” may sound awkward, it’s the only wording that statisticians accept. Be careful not to say you “prove” or “accept” the null hypothesis.

Null hypotheses often include phrases such as “no effect”, “no difference”, or “no relationship”. When written in mathematical terms, they always include an equality (usually =, but sometimes ≥ or ≤).

Examples of null hypotheses

The table below gives examples of research questions and null hypotheses. There’s always more than one way to answer a research question, but these null hypotheses can help you get started.

*Note that some researchers prefer to always write the null hypothesis in terms of “no effect” and “=”. It would be fine to say that daily meditation has no effect on the incidence of depression and p 1 = p 2 .

The alternative hypothesis (H A ) is the other answer to your research question . It claims that there’s an effect in the population.

Often, your alternative hypothesis is the same as your research hypothesis. In other words, it’s the claim that you expect or hope will be true.

The alternative hypothesis is the complement to the null hypothesis. Null and alternative hypotheses are exhaustive, meaning that together they cover every possible outcome. They are also mutually exclusive, meaning that only one can be true at a time.

Alternative hypotheses often include phrases such as “an effect”, “a difference”, or “a relationship”. When alternative hypotheses are written in mathematical terms, they always include an inequality (usually ≠, but sometimes > or <). As with null hypotheses, there are many acceptable ways to phrase an alternative hypothesis.

Examples of alternative hypotheses

The table below gives examples of research questions and alternative hypotheses to help you get started with formulating your own.

Null and alternative hypotheses are similar in some ways:

• They’re both answers to the research question
• They both make claims about the population
• They’re both evaluated by statistical tests.

However, there are important differences between the two types of hypotheses, summarized in the following table.

To help you write your hypotheses, you can use the template sentences below. If you know which statistical test you’re going to use, you can use the test-specific template sentences. Otherwise, you can use the general template sentences.

The only thing you need to know to use these general template sentences are your dependent and independent variables. To write your research question, null hypothesis, and alternative hypothesis, fill in the following sentences with your variables:

Does independent variable affect dependent variable ?

• Null hypothesis (H 0 ): Independent variable does not affect dependent variable .
• Alternative hypothesis (H A ): Independent variable affects dependent variable .

Test-specific

Once you know the statistical test you’ll be using, you can write your hypotheses in a more precise and mathematical way specific to the test you chose. The table below provides template sentences for common statistical tests.

Note: The template sentences above assume that you’re performing one-tailed tests . One-tailed tests are appropriate for most studies.

The null hypothesis is often abbreviated as H 0 . When the null hypothesis is written using mathematical symbols, it always includes an equality symbol (usually =, but sometimes ≥ or ≤).

The alternative hypothesis is often abbreviated as H a or H 1 . When the alternative hypothesis is written using mathematical symbols, it always includes an inequality symbol (usually ≠, but sometimes < or >).

A research hypothesis is your proposed answer to your research question. The research hypothesis usually includes an explanation (‘ x affects y because …’).

A statistical hypothesis, on the other hand, is a mathematical statement about a population parameter. Statistical hypotheses always come in pairs: the null and alternative hypotheses. In a well-designed study , the statistical hypotheses correspond logically to the research hypothesis.

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Module 9: Hypothesis Testing With One Sample

Null and alternative hypotheses, learning outcomes.

• Describe hypothesis testing in general and in practice

The actual test begins by considering two  hypotheses . They are called the null hypothesis and the alternative hypothesis . These hypotheses contain opposing viewpoints.

H 0 : The null hypothesis: It is a statement about the population that either is believed to be true or is used to put forth an argument unless it can be shown to be incorrect beyond a reasonable doubt.

H a : The alternative hypothesis : It is a claim about the population that is contradictory to H 0 and what we conclude when we reject H 0 .

Since the null and alternative hypotheses are contradictory, you must examine evidence to decide if you have enough evidence to reject the null hypothesis or not. The evidence is in the form of sample data.

After you have determined which hypothesis the sample supports, you make adecision. There are two options for a  decision . They are “reject H 0 ” if the sample information favors the alternative hypothesis or “do not reject H 0 ” or “decline to reject H 0 ” if the sample information is insufficient to reject the null hypothesis.

Mathematical Symbols Used in  H 0 and H a :

H 0 always has a symbol with an equal in it. H a never has a symbol with an equal in it. The choice of symbol depends on the wording of the hypothesis test. However, be aware that many researchers (including one of the co-authors in research work) use = in the null hypothesis, even with > or < as the symbol in the alternative hypothesis. This practice is acceptable because we only make the decision to reject or not reject the null hypothesis.

H 0 : No more than 30% of the registered voters in Santa Clara County voted in the primary election. p ≤ 30

H a : More than 30% of the registered voters in Santa Clara County voted in the primary election. p > 30

A medical trial is conducted to test whether or not a new medicine reduces cholesterol by 25%. State the null and alternative hypotheses.

H 0 : The drug reduces cholesterol by 25%. p = 0.25

H a : The drug does not reduce cholesterol by 25%. p ≠ 0.25

We want to test whether the mean GPA of students in American colleges is different from 2.0 (out of 4.0). The null and alternative hypotheses are:

H 0 : μ = 2.0

H a : μ ≠ 2.0

We want to test whether the mean height of eighth graders is 66 inches. State the null and alternative hypotheses. Fill in the correct symbol (=, ≠, ≥, <, ≤, >) for the null and alternative hypotheses. H 0 : μ __ 66 H a : μ __ 66

• H 0 : μ = 66
• H a : μ ≠ 66

We want to test if college students take less than five years to graduate from college, on the average. The null and alternative hypotheses are:

H 0 : μ ≥ 5

H a : μ < 5

We want to test if it takes fewer than 45 minutes to teach a lesson plan. State the null and alternative hypotheses. Fill in the correct symbol ( =, ≠, ≥, <, ≤, >) for the null and alternative hypotheses. H 0 : μ __ 45 H a : μ __ 45

• H 0 : μ ≥ 45
• H a : μ < 45

In an issue of U.S. News and World Report , an article on school standards stated that about half of all students in France, Germany, and Israel take advanced placement exams and a third pass. The same article stated that 6.6% of U.S. students take advanced placement exams and 4.4% pass. Test if the percentage of U.S. students who take advanced placement exams is more than 6.6%. State the null and alternative hypotheses.

H 0 : p ≤ 0.066

H a : p > 0.066

On a state driver’s test, about 40% pass the test on the first try. We want to test if more than 40% pass on the first try. Fill in the correct symbol (=, ≠, ≥, <, ≤, >) for the null and alternative hypotheses. H 0 : p __ 0.40 H a : p __ 0.40

• H 0 : p = 0.40
• H a : p > 0.40

Concept Review

In a  hypothesis test , sample data is evaluated in order to arrive at a decision about some type of claim. If certain conditions about the sample are satisfied, then the claim can be evaluated for a population. In a hypothesis test, we: Evaluate the null hypothesis , typically denoted with H 0 . The null is not rejected unless the hypothesis test shows otherwise. The null statement must always contain some form of equality (=, ≤ or ≥) Always write the alternative hypothesis , typically denoted with H a or H 1 , using less than, greater than, or not equals symbols, i.e., (≠, >, or <). If we reject the null hypothesis, then we can assume there is enough evidence to support the alternative hypothesis. Never state that a claim is proven true or false. Keep in mind the underlying fact that hypothesis testing is based on probability laws; therefore, we can talk only in terms of non-absolute certainties.

Formula Review

H 0 and H a are contradictory.

• OpenStax, Statistics, Null and Alternative Hypotheses. Provided by : OpenStax. Located at : http://cnx.org/contents/[email protected]:58/Introductory_Statistics . License : CC BY: Attribution
• Introductory Statistics . Authored by : Barbara Illowski, Susan Dean. Provided by : Open Stax. Located at : http://cnx.org/contents/[email protected] . License : CC BY: Attribution . License Terms : Download for free at http://cnx.org/contents/[email protected]
• Simple hypothesis testing | Probability and Statistics | Khan Academy. Authored by : Khan Academy. Located at : https://youtu.be/5D1gV37bKXY . License : All Rights Reserved . License Terms : Standard YouTube License

9.1 Null and Alternative Hypotheses

The actual test begins by considering two hypotheses . They are called the null hypothesis and the alternative hypothesis . These hypotheses contain opposing viewpoints.

H 0 , the — null hypothesis: a statement of no difference between sample means or proportions or no difference between a sample mean or proportion and a population mean or proportion. In other words, the difference equals 0.

H a —, the alternative hypothesis: a claim about the population that is contradictory to H 0 and what we conclude when we reject H 0 .

Since the null and alternative hypotheses are contradictory, you must examine evidence to decide if you have enough evidence to reject the null hypothesis or not. The evidence is in the form of sample data.

After you have determined which hypothesis the sample supports, you make a decision. There are two options for a decision. They are reject H 0 if the sample information favors the alternative hypothesis or do not reject H 0 or decline to reject H 0 if the sample information is insufficient to reject the null hypothesis.

Mathematical Symbols Used in H 0 and H a :

H 0 always has a symbol with an equal in it. H a never has a symbol with an equal in it. The choice of symbol depends on the wording of the hypothesis test. However, be aware that many researchers use = in the null hypothesis, even with > or < as the symbol in the alternative hypothesis. This practice is acceptable because we only make the decision to reject or not reject the null hypothesis.

Example 9.1

H 0 : No more than 30 percent of the registered voters in Santa Clara County voted in the primary election. p ≤ 30 H a : More than 30 percent of the registered voters in Santa Clara County voted in the primary election. p > 30

A medical trial is conducted to test whether or not a new medicine reduces cholesterol by 25 percent. State the null and alternative hypotheses.

Example 9.2

We want to test whether the mean GPA of students in American colleges is different from 2.0 (out of 4.0). The null and alternative hypotheses are the following: H 0 : μ = 2.0 H a : μ ≠ 2.0

We want to test whether the mean height of eighth graders is 66 inches. State the null and alternative hypotheses. Fill in the correct symbol (=, ≠, ≥, <, ≤, >) for the null and alternative hypotheses.

• H 0 : μ __ 66
• H a : μ __ 66

Example 9.3

We want to test if college students take fewer than five years to graduate from college, on the average. The null and alternative hypotheses are the following: H 0 : μ ≥ 5 H a : μ < 5

We want to test if it takes fewer than 45 minutes to teach a lesson plan. State the null and alternative hypotheses. Fill in the correct symbol ( =, ≠, ≥, <, ≤, >) for the null and alternative hypotheses.

• H 0 : μ __ 45
• H a : μ __ 45

Example 9.4

An article on school standards stated that about half of all students in France, Germany, and Israel take advanced placement exams and a third of the students pass. The same article stated that 6.6 percent of U.S. students take advanced placement exams and 4.4 percent pass. Test if the percentage of U.S. students who take advanced placement exams is more than 6.6 percent. State the null and alternative hypotheses. H 0 : p ≤ 0.066 H a : p > 0.066

On a state driver’s test, about 40 percent pass the test on the first try. We want to test if more than 40 percent pass on the first try. Fill in the correct symbol (=, ≠, ≥, <, ≤, >) for the null and alternative hypotheses.

• H 0 : p __ 0.40
• H a : p __ 0.40

Collaborative Exercise

Bring to class a newspaper, some news magazines, and some internet articles. In groups, find articles from which your group can write null and alternative hypotheses. Discuss your hypotheses with the rest of the class.

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Statistics By Jim

Making statistics intuitive

Null Hypothesis: Definition, Rejecting & Examples

By Jim Frost 6 Comments

What is a Null Hypothesis?

The null hypothesis in statistics states that there is no difference between groups or no relationship between variables. It is one of two mutually exclusive hypotheses about a population in a hypothesis test.

• Null Hypothesis H 0 : No effect exists in the population.
• Alternative Hypothesis H A : The effect exists in the population.

In every study or experiment, researchers assess an effect or relationship. This effect can be the effectiveness of a new drug, building material, or other intervention that has benefits. There is a benefit or connection that the researchers hope to identify. Unfortunately, no effect may exist. In statistics, we call this lack of an effect the null hypothesis. Researchers assume that this notion of no effect is correct until they have enough evidence to suggest otherwise, similar to how a trial presumes innocence.

In this context, the analysts don’t necessarily believe the null hypothesis is correct. In fact, they typically want to reject it because that leads to more exciting finds about an effect or relationship. The new vaccine works!

You can think of it as the default theory that requires sufficiently strong evidence to reject. Like a prosecutor, researchers must collect sufficient evidence to overturn the presumption of no effect. Investigators must work hard to set up a study and a data collection system to obtain evidence that can reject the null hypothesis.

Related post : What is an Effect in Statistics?

Null Hypothesis Examples

Null hypotheses start as research questions that the investigator rephrases as a statement indicating there is no effect or relationship.

After reading these examples, you might think they’re a bit boring and pointless. However, the key is to remember that the null hypothesis defines the condition that the researchers need to discredit before suggesting an effect exists.

Let’s see how you reject the null hypothesis and get to those more exciting findings!

When to Reject the Null Hypothesis

So, you want to reject the null hypothesis, but how and when can you do that? To start, you’ll need to perform a statistical test on your data. The following is an overview of performing a study that uses a hypothesis test.

The first step is to devise a research question and the appropriate null hypothesis. After that, the investigators need to formulate an experimental design and data collection procedures that will allow them to gather data that can answer the research question. Then they collect the data. For more information about designing a scientific study that uses statistics, read my post 5 Steps for Conducting Studies with Statistics .

After data collection is complete, statistics and hypothesis testing enter the picture. Hypothesis testing takes your sample data and evaluates how consistent they are with the null hypothesis. The p-value is a crucial part of the statistical results because it quantifies how strongly the sample data contradict the null hypothesis.

When the sample data provide sufficient evidence, you can reject the null hypothesis. In a hypothesis test, this process involves comparing the p-value to your significance level .

Rejecting the Null Hypothesis

Reject the null hypothesis when the p-value is less than or equal to your significance level. Your sample data favor the alternative hypothesis, which suggests that the effect exists in the population. For a mnemonic device, remember—when the p-value is low, the null must go!

When you can reject the null hypothesis, your results are statistically significant. Learn more about Statistical Significance: Definition & Meaning .

Failing to Reject the Null Hypothesis

Conversely, when the p-value is greater than your significance level, you fail to reject the null hypothesis. The sample data provides insufficient data to conclude that the effect exists in the population. When the p-value is high, the null must fly!

Note that failing to reject the null is not the same as proving it. For more information about the difference, read my post about Failing to Reject the Null .

That’s a very general look at the process. But I hope you can see how the path to more exciting findings depends on being able to rule out the less exciting null hypothesis that states there’s nothing to see here!

Let’s move on to learning how to write the null hypothesis for different types of effects, relationships, and tests.

Related posts : How Hypothesis Tests Work and Interpreting P-values

How to Write a Null Hypothesis

The null hypothesis varies by the type of statistic and hypothesis test. Remember that inferential statistics use samples to draw conclusions about populations. Consequently, when you write a null hypothesis, it must make a claim about the relevant population parameter . Further, that claim usually indicates that the effect does not exist in the population. Below are typical examples of writing a null hypothesis for various parameters and hypothesis tests.

Related posts : Descriptive vs. Inferential Statistics and Populations, Parameters, and Samples in Inferential Statistics

Group Means

T-tests and ANOVA assess the differences between group means. For these tests, the null hypothesis states that there is no difference between group means in the population. In other words, the experimental conditions that define the groups do not affect the mean outcome. Mu (µ) is the population parameter for the mean, and you’ll need to include it in the statement for this type of study.

For example, an experiment compares the mean bone density changes for a new osteoporosis medication. The control group does not receive the medicine, while the treatment group does. The null states that the mean bone density changes for the control and treatment groups are equal.

• Null Hypothesis H 0 : Group means are equal in the population: µ 1 = µ 2 , or µ 1 – µ 2 = 0
• Alternative Hypothesis H A : Group means are not equal in the population: µ 1 ≠ µ 2 , or µ 1 – µ 2 ≠ 0.

Group Proportions

Proportions tests assess the differences between group proportions. For these tests, the null hypothesis states that there is no difference between group proportions. Again, the experimental conditions did not affect the proportion of events in the groups. P is the population proportion parameter that you’ll need to include.

For example, a vaccine experiment compares the infection rate in the treatment group to the control group. The treatment group receives the vaccine, while the control group does not. The null states that the infection rates for the control and treatment groups are equal.

• Null Hypothesis H 0 : Group proportions are equal in the population: p 1 = p 2 .
• Alternative Hypothesis H A : Group proportions are not equal in the population: p 1 ≠ p 2 .

Correlation and Regression Coefficients

Some studies assess the relationship between two continuous variables rather than differences between groups.

In these studies, analysts often use either correlation or regression analysis . For these tests, the null states that there is no relationship between the variables. Specifically, it says that the correlation or regression coefficient is zero. As one variable increases, there is no tendency for the other variable to increase or decrease. Rho (ρ) is the population correlation parameter and beta (β) is the regression coefficient parameter.

For example, a study assesses the relationship between screen time and test performance. The null states that there is no correlation between this pair of variables. As screen time increases, test performance does not tend to increase or decrease.

• Null Hypothesis H 0 : The correlation in the population is zero: ρ = 0.
• Alternative Hypothesis H A : The correlation in the population is not zero: ρ ≠ 0.

For all these cases, the analysts define the hypotheses before the study. After collecting the data, they perform a hypothesis test to determine whether they can reject the null hypothesis.

The preceding examples are all for two-tailed hypothesis tests. To learn about one-tailed tests and how to write a null hypothesis for them, read my post One-Tailed vs. Two-Tailed Tests .

Related post : Understanding Correlation

Neyman, J; Pearson, E. S. (January 1, 1933).  On the Problem of the most Efficient Tests of Statistical Hypotheses .  Philosophical Transactions of the Royal Society A .  231  (694–706): 289–337.

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Reader Interactions

January 11, 2024 at 2:57 pm

Thanks for the reply.

January 10, 2024 at 1:23 pm

Hi Jim, In your comment you state that equivalence test null and alternate hypotheses are reversed. For hypothesis tests of data fits to a probability distribution, the null hypothesis is that the probability distribution fits the data. Is this correct?

January 10, 2024 at 2:15 pm

Those two separate things, equivalence testing and normality tests. But, yes, you’re correct for both.

Hypotheses are switched for equivalence testing. You need to “work” (i.e., collect a large sample of good quality data) to be able to reject the null that the groups are different to be able to conclude they’re the same.

With typical hypothesis tests, if you have low quality data and a low sample size, you’ll fail to reject the null that they’re the same, concluding they’re equivalent. But that’s more a statement about the low quality and small sample size than anything to do with the groups being equal.

So, equivalence testing make you work to obtain a finding that the groups are the same (at least within some amount you define as a trivial difference).

For normality testing, and other distribution tests, the null states that the data follow the distribution (normal or whatever). If you reject the null, you have sufficient evidence to conclude that your sample data don’t follow the probability distribution. That’s a rare case where you hope to fail to reject the null. And it suffers from the problem I describe above where you might fail to reject the null simply because you have a small sample size. In that case, you’d conclude the data follow the probability distribution but it’s more that you don’t have enough data for the test to register the deviation. In this scenario, if you had a larger sample size, you’d reject the null and conclude it doesn’t follow that distribution.

I don’t know of any equivalence testing type approach for distribution fit tests where you’d need to work to show the data follow a distribution, although I haven’t looked for one either!

February 20, 2022 at 9:26 pm

Is a null hypothesis regularly (always) stated in the negative? “there is no” or “does not”

February 23, 2022 at 9:21 pm

Typically, the null hypothesis includes an equal sign. The null hypothesis states that the population parameter equals a particular value. That value is usually one that represents no effect. In the case of a one-sided hypothesis test, the null still contains an equal sign but it’s “greater than or equal to” or “less than or equal to.” If you wanted to translate the null hypothesis from its native mathematical expression, you could use the expression “there is no effect.” But the mathematical form more specifically states what it’s testing.

It’s the alternative hypothesis that typically contains does not equal.

There are some exceptions. For example, in an equivalence test where the researchers want to show that two things are equal, the null hypothesis states that they’re not equal.

In short, the null hypothesis states the condition that the researchers hope to reject. They need to work hard to set up an experiment and data collection that’ll gather enough evidence to be able to reject the null condition.

February 15, 2022 at 9:32 am

Dear sir I always read your notes on Research methods.. Kindly tell is there any available Book on all these..wonderfull Urgent

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Hypothesis Testing: Null Hypothesis and Alternative Hypothesis

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Figuring out exactly what the null hypothesis and the alternative hypotheses are is not a walk in the park. Hypothesis testing is based on the knowledge that you can acquire by going over what we have previously covered about statistics in our blog.

So, if you don’t want to have a hard time keeping up, make sure you have read all the tutorials about confidence intervals , distributions , z-tables and t-tables .

We've also made a video on null hypothesis vs alternative hypothesis - you can watch it below or just scroll down if you prefer reading.

Confidence intervals provide us with an estimation of where the parameters are located. You can obtain them with our confidence interval calculator and learn more about them in the related article.

However, when we are making a decision, we need a yes or no answer. The correct approach, in this case, is to use a test .

Here we will start learning about one of the fundamental tasks in statistics - hypothesis testing !

The Hypothesis Testing Process

  First off, let’s talk about data-driven decision-making. It consists of the following steps:

• First, we must formulate a hypothesis .
• After doing that, we have to find the right test for our hypothesis .
• Then, we execute the test.
• Finally, we make a decision based on the result.

Let’s start from the beginning.

What is a Hypothesis?

Though there are many ways to define it, the most intuitive must be:

“A hypothesis is an idea that can be tested.”

This is not the formal definition, but it explains the point very well.

So, if we say that apples in New York are expensive, this is an idea or a statement. However, it is not testable, until we have something to compare it with.

For instance, if we define expensive as: any price higher than $1.75 dollars per pound, then it immediately becomes a hypothesis .

What Cannot Be a Hypothesis?

An example may be: would the USA do better or worse under a Clinton administration, compared to a Trump administration? Statistically speaking, this is an idea , but there is no data to test it. Therefore, it cannot be a hypothesis of a statistical test.

Actually, it is more likely to be a topic of another discipline.

Conversely, in statistics, we may compare different US presidencies that have already been completed. For example, the Obama administration and the Bush administration, as we have data on both.

A Two-Sided Test

Alright, let’s get out of politics and get into hypotheses . Here’s a simple topic that CAN be tested.

According to Glassdoor (the popular salary information website), the mean data scientist salary in the US is 113,000 dollars.

So, we want to test if their estimate is correct.

The Null and Alternative Hypotheses

There are two hypotheses that are made: the null hypothesis , denoted H 0 , and the alternative hypothesis , denoted H 1 or H A .

The null hypothesis is the one to be tested and the alternative is everything else. In our example:

The null hypothesis would be: The mean data scientist salary is 113,000 dollars.

While the alternative : The mean data scientist salary is not 113,000 dollars.

Author's note: If you're interested in a data scientist career, check out our articles Data Scientist Career Path , 5 Business Basics for Data Scientists , Data Science Interview Questions , and 15 Data Science Consulting Companies Hiring Now .

An Example of a One-Sided Test

You can also form one-sided or one-tailed tests.

Say your friend, Paul, told you that he thinks data scientists earn more than 125,000 dollars per year. You doubt him, so you design a test to see who’s right.

The null hypothesis of this test would be: The mean data scientist salary is more than 125,000 dollars.

The alternative will cover everything else, thus: The mean data scientist salary is less than or equal to 125,000 dollars.

Important: The outcomes of tests refer to the population parameter rather than the sample statistic! So, the result that we get is for the population.

Important: Another crucial consideration is that, generally, the researcher is trying to reject the null hypothesis . Think about the null hypothesis as the status quo and the alternative as the change or innovation that challenges that status quo. In our example, Paul was representing the status quo, which we were challenging.

Let’s go over it once more. In statistics, the null hypothesis is the statement we are trying to reject. Therefore, the null hypothesis is the present state of affairs, while the alternative is our personal opinion.

Why Hypothesis Testing Works

Right now, you may be feeling a little puzzled. This is normal because this whole concept is counter-intuitive at the beginning. However, there is an extremely easy way to continue your journey of exploring it. By diving into the linked tutorial, you will find out why hypothesis testing actually works.

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Next Tutorial:  Hypothesis Testing: Significance Level and Rejection Region

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Null vs. Alternative Hypothesis

04.28.2023 • 5 min read

Sarah Thomas

Subject Matter Expert

Learn about a null versus alternative hypothesis and what they show with examples for each. Also go over the main differences and similarities between them.

In This Article

What Is a Null Hypothesis?

What is an alternative hypothesis, outcomes of a hypothesis test.

Main Differences Between Null & Alternative Hypothesis

Similarities Between Null & Alternative Hypothesis

Hypothesis Testing & Errors

In statistics, you’ll draw insights or “inferences” about population parameters using data from a sample. This process is called inferential statistics.

To make statistical inferences, you need to determine if you have enough evidence to support a certain hypothesis about the population. This is where null and alternative hypotheses come into play!

In this article, we’ll explain the differences between these two types of hypotheses, and we’ll explain the role they play in hypothesis testing.

Imagine you want to know what percent of Americans are vegetarians. You find a Gallup poll claiming ‌5% of the population was vegetarian in 2018, but your intuition tells you vegetarianism is on the rise and that ‌far more than 5% of Americans are vegetarian today.

To investigate further, you collect your own sample data by surveying 1,000 randomly selected Americans. You’ll use this random sample to determine whether it’s likely ‌the true population proportion of vegetarians is, in fact, 5% (as the Gallup data suggests) or whether it could be the case that the percentage of vegetarians is now higher.

Notice ‌that your investigation involves two rival hypotheses about the population. One hypothesis is that the proportion of vegetarians is 5%. The other hypothesis is that the proportion of vegetarians is greater than 5%. In statistics, we would call the first hypothesis the null hypothesis, and the second hypothesis the alternative hypothesis. The null hypothesis ( H 0 H_0 H 0 ​ ) represents the status quo or what is assumed to be true about the population at the start of your investigation.

Null Hypothesis

In hypothesis testing, the null hypothesis ( H 0 H_0 H 0 ​ ) is the default hypothesis.

It's what the status quo assumes to be true about the population.

The alternative hypothesis ( H a H_a H a ​ or H 1 H_1 H 1 ​ ) is the hypothesis that stands contrary to the null hypothesis. The alternative hypothesis ‌represents the research hypothesis—what you as the statistician are trying to prove with your data .

In medical studies, where scientists are trying to demonstrate whether a treatment has a significant effect on patient outcomes, the alternative hypothesis represents the hypothesis that the treatment does have an effect, while the null hypothesis represents the assumption that the treatment has no effect.

Alternative Hypothesis

The alternative hypothesis ( H a H_a H a ​ or H 1 H_1 H 1 ​ ) is the hypothesis being proposed in opposition to the null hypothesis.

Examples of Null and Alternative Hypotheses

In a hypothesis test, the null and alternative hypotheses must be mutually exclusive statements, meaning both hypotheses cannot be true at the same time. For example, if the null hypothesis includes an equal sign, the alternative hypothesis must state that the values being mentioned are “not equal” in some way.

Your hypotheses will also depend on the formulation of your test—are you running a one-sample T-test, a two-sample T-test, F-test for ANOVA , or a Chi-squared test? It also matters whether you are conducting a directional one-tailed test or a nondirectional two-tailed test.

Example 1: Two-Tailed T-test

Null Hypothesis: The population mean is equal to some number, x. 𝝁 = x

Alternative Hypothesis: The population mean is not equal to x. 𝝁 ≠ x

Example 2: One-tailed T-test (Right-Tailed)

Null Hypothesis: The population mean is less than or equal to some number, x. 𝝁 ≤ x Alternative Hypothesis: The population mean is greater than x. 𝝁 > x

Example 3: One-tailed T-test (Left-Tailed)

Null Hypothesis: The population mean is greater than or equal to some number, x. 𝝁 ≥ x

Alternative Hypothesis: The population mean is less than x. 𝝁 < x

By the end of a hypothesis test, you will have reached one of two conclusions.

You will run into either 2 outcomes:

Fail to reject the null hypothesis on the grounds that there's insufficient evidence to move away from the null hypothesis

Reject the null hypothesis in favor of the alternative.

If you’re ‌confused about the outcomes of a hypothesis test, a good analogy is a jury trial. In a jury trial, the defendant is innocent until proven guilty. To reach a verdict of guilt, the jury must find strong evidence (beyond a reasonable doubt) that the defendant committed the crime.

This is analogous to a statistician who must assume the null hypothesis is true unless they can uncover strong evidence ( a p-value less than or equal to the significance level) in support of the alternative hypothesis.

Notice also, that a jury never concludes a defendant is innocent—only that the defendant is guilty or not guilty. This is similar to how we never conclude that the null hypothesis is true. In a hypothesis test, we never conclude ‌that the null hypothesis is true. We can only “reject” the null hypothesis or “fail to reject” it.

In this video, let’s look at the jury example again, the reasoning behind hypothesis testing, and how to form a test. It starts by stating your null and alternative hypotheses.

Main Differences Between Null and Alternative Hypothesis

Here is a summary of the key differences between the null and the alternative hypothesis test.

The null hypothesis represents the status quo; the alternative hypothesis represents an alternative statement about the population.

The null and the alternative are mutually exclusive statements, meaning both statements cannot be true at the same time.

In a medical study, the null hypothesis represents the assumption that a treatment has no statistically significant effect on the outcome being studied. The alternative hypothesis represents the belief that the treatment does have an effect.

The null hypothesis is denoted by H_0 ; the alternative hypothesis is denoted by H_a H_1

You “fail to reject” the null hypothesis when the p-value is larger than the significance level. You “reject” the null hypothesis in favor of the alternative hypothesis when the p-value is less than or equal to your test’s significance level.

Similarities Between Null and Alternative Hypothesis

The similarities between the null and alternative hypotheses are as follows.

Both the null and the alternative are statements about the same underlying data.

Both statements provide a possible answer to a statistician’s research question.

The same hypothesis test will provide evidence for or against the null and alternative hypotheses.

Hypothesis Testing and Errors

Always remember that statistical inference provides you with inferences based on probability rather than hard truths. Anytime you conduct a hypothesis test, there is a chance that you’ll reach the wrong conclusion about your data.

In statistics, we categorize these wrong conclusions into two types of errors:

Type I Errors

Type II Errors

Type I Error (ɑ)

A Type I error occurs when you reject the null hypothesis when, in fact, the null hypothesis is true. This is sometimes called a false positive and is analogous to a jury that falsely convicts an innocent defendant. The probability of making this type of error is represented by alpha, ɑ.

Type II Error (ꞵ)

A Type II error occurs when you fail to reject the null hypothesis when, in fact, the null hypothesis is false. This is sometimes called a false negative and is analogous to a jury that reaches a verdict of “not guilty,” when, in fact, the defendant has committed the crime. The probability of making this type of error is represented by beta, ꞵ.

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10.1 - setting the hypotheses: examples.

A significance test examines whether the null hypothesis provides a plausible explanation of the data. The null hypothesis itself does not involve the data. It is a statement about a parameter (a numerical characteristic of the population). These population values might be proportions or means or differences between means or proportions or correlations or odds ratios or any other numerical summary of the population. The alternative hypothesis is typically the research hypothesis of interest. Here are some examples.

Example 10.2: Hypotheses with One Sample of One Categorical Variable Section  

About 10% of the human population is left-handed. Suppose a researcher at Penn State speculates that students in the College of Arts and Architecture are more likely to be left-handed than people found in the general population. We only have one sample since we will be comparing a population proportion based on a sample value to a known population value.

• Research Question : Are artists more likely to be left-handed than people found in the general population?
• Response Variable : Classification of the student as either right-handed or left-handed

State Null and Alternative Hypotheses

• Null Hypothesis : Students in the College of Arts and Architecture are no more likely to be left-handed than people in the general population (population percent of left-handed students in the College of Art and Architecture = 10% or p = .10).
• Alternative Hypothesis : Students in the College of Arts and Architecture are more likely to be left-handed than people in the general population (population percent of left-handed students in the College of Arts and Architecture > 10% or p > .10). This is a one-sided alternative hypothesis.

Example 10.3: Hypotheses with One Sample of One Measurement Variable Section  

A generic brand of the anti-histamine Diphenhydramine markets a capsule with a 50 milligram dose. The manufacturer is worried that the machine that fills the capsules has come out of calibration and is no longer creating capsules with the appropriate dosage.

• Research Question : Does the data suggest that the population mean dosage of this brand is different than 50 mg?
• Response Variable : dosage of the active ingredient found by a chemical assay.
• Null Hypothesis : On the average, the dosage sold under this brand is 50 mg (population mean dosage = 50 mg).
• Alternative Hypothesis : On the average, the dosage sold under this brand is not 50 mg (population mean dosage ≠ 50 mg). This is a two-sided alternative hypothesis.

Example 10.4: Hypotheses with Two Samples of One Categorical Variable Section  

Many people are starting to prefer vegetarian meals on a regular basis. Specifically, a researcher believes that females are more likely than males to eat vegetarian meals on a regular basis.

• Research Question : Does the data suggest that females are more likely than males to eat vegetarian meals on a regular basis?
• Response Variable : Classification of whether or not a person eats vegetarian meals on a regular basis
• Explanatory (Grouping) Variable: Sex
• Null Hypothesis : There is no sex effect regarding those who eat vegetarian meals on a regular basis (population percent of females who eat vegetarian meals on a regular basis = population percent of males who eat vegetarian meals on a regular basis or p females = p males ).
• Alternative Hypothesis : Females are more likely than males to eat vegetarian meals on a regular basis (population percent of females who eat vegetarian meals on a regular basis > population percent of males who eat vegetarian meals on a regular basis or p females > p males ). This is a one-sided alternative hypothesis.

Example 10.5: Hypotheses with Two Samples of One Measurement Variable Section  

Obesity is a major health problem today. Research is starting to show that people may be able to lose more weight on a low carbohydrate diet than on a low fat diet.

• Research Question : Does the data suggest that, on the average, people are able to lose more weight on a low carbohydrate diet than on a low fat diet?
• Response Variable : Weight loss (pounds)
• Explanatory (Grouping) Variable : Type of diet
• Null Hypothesis : There is no difference in the mean amount of weight loss when comparing a low carbohydrate diet with a low fat diet (population mean weight loss on a low carbohydrate diet = population mean weight loss on a low fat diet).
• Alternative Hypothesis : The mean weight loss should be greater for those on a low carbohydrate diet when compared with those on a low fat diet (population mean weight loss on a low carbohydrate diet > population mean weight loss on a low fat diet). This is a one-sided alternative hypothesis.

Example 10.6: Hypotheses about the relationship between Two Categorical Variables Section  

• Research Question : Do the odds of having a stroke increase if you inhale second hand smoke ? A case-control study of non-smoking stroke patients and controls of the same age and occupation are asked if someone in their household smokes.
• Variables : There are two different categorical variables (Stroke patient vs control and whether the subject lives in the same household as a smoker). Living with a smoker (or not) is the natural explanatory variable and having a stroke (or not) is the natural response variable in this situation.
• Null Hypothesis : There is no relationship between whether or not a person has a stroke and whether or not a person lives with a smoker (odds ratio between stroke and second-hand smoke situation is = 1).
• Alternative Hypothesis : There is a relationship between whether or not a person has a stroke and whether or not a person lives with a smoker (odds ratio between stroke and second-hand smoke situation is > 1). This is a one-tailed alternative.

This research question might also be addressed like example 11.4 by making the hypotheses about comparing the proportion of stroke patients that live with smokers to the proportion of controls that live with smokers.

Example 10.7: Hypotheses about the relationship between Two Measurement Variables Section  

• Research Question : A financial analyst believes there might be a positive association between the change in a stock's price and the amount of the stock purchased by non-management employees the previous day (stock trading by management being under "insider-trading" regulatory restrictions).
• Variables : Daily price change information (the response variable) and previous day stock purchases by non-management employees (explanatory variable). These are two different measurement variables.
• Null Hypothesis : The correlation between the daily stock price change (\$) and the daily stock purchases by non-management employees (\$) = 0.
• Alternative Hypothesis : The correlation between the daily stock price change (\$) and the daily stock purchases by non-management employees (\$) > 0. This is a one-sided alternative hypothesis.

Example 10.8: Hypotheses about comparing the relationship between Two Measurement Variables in Two Samples Section  

• Research Question : Is there a linear relationship between the amount of the bill (\$) at a restaurant and the tip (\$) that was left. Is the strength of this association different for family restaurants than for fine dining restaurants?
• Variables : There are two different measurement variables. The size of the tip would depend on the size of the bill so the amount of the bill would be the explanatory variable and the size of the tip would be the response variable.
• Null Hypothesis : The correlation between the amount of the bill (\$) at a restaurant and the tip (\$) that was left is the same at family restaurants as it is at fine dining restaurants.
• Alternative Hypothesis : The correlation between the amount of the bill (\$) at a restaurant and the tip (\$) that was left is the difference at family restaurants then it is at fine dining restaurants. This is a two-sided alternative hypothesis.

Null Hypothesis and Alternative Hypothesis

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Hypothesis testing involves the careful construction of two statements: the null hypothesis and the alternative hypothesis. These hypotheses can look very similar but are actually different.

How do we know which hypothesis is the null and which one is the alternative? We will see that there are a few ways to tell the difference.

The Null Hypothesis

The null hypothesis reflects that there will be no observed effect in our experiment. In a mathematical formulation of the null hypothesis, there will typically be an equal sign. This hypothesis is denoted by H 0 .

The null hypothesis is what we attempt to find evidence against in our hypothesis test. We hope to obtain a small enough p-value that it is lower than our level of significance alpha and we are justified in rejecting the null hypothesis. If our p-value is greater than alpha, then we fail to reject the null hypothesis.

If the null hypothesis is not rejected, then we must be careful to say what this means. The thinking on this is similar to a legal verdict. Just because a person has been declared "not guilty", it does not mean that he is innocent. In the same way, just because we failed to reject a null hypothesis it does not mean that the statement is true.

For example, we may want to investigate the claim that despite what convention has told us, the mean adult body temperature is not the accepted value of 98.6 degrees Fahrenheit . The null hypothesis for an experiment to investigate this is “The mean adult body temperature for healthy individuals is 98.6 degrees Fahrenheit.” If we fail to reject the null hypothesis, then our working hypothesis remains that the average adult who is healthy has a temperature of 98.6 degrees. We do not prove that this is true.

If we are studying a new treatment, the null hypothesis is that our treatment will not change our subjects in any meaningful way. In other words, the treatment will not produce any effect in our subjects.

The Alternative Hypothesis

The alternative or experimental hypothesis reflects that there will be an observed effect for our experiment. In a mathematical formulation of the alternative hypothesis, there will typically be an inequality, or not equal to symbol. This hypothesis is denoted by either H a or by H 1 .

The alternative hypothesis is what we are attempting to demonstrate in an indirect way by the use of our hypothesis test. If the null hypothesis is rejected, then we accept the alternative hypothesis. If the null hypothesis is not rejected, then we do not accept the alternative hypothesis. Going back to the above example of mean human body temperature, the alternative hypothesis is “The average adult human body temperature is not 98.6 degrees Fahrenheit.”

If we are studying a new treatment, then the alternative hypothesis is that our treatment does, in fact, change our subjects in a meaningful and measurable way.

The following set of negations may help when you are forming your null and alternative hypotheses. Most technical papers rely on just the first formulation, even though you may see some of the others in a statistics textbook.

• Null hypothesis: “ x is equal to y .” Alternative hypothesis “ x is not equal to y .”
• Null hypothesis: “ x is at least y .” Alternative hypothesis “ x is less than y .”
• Null hypothesis: “ x is at most y .” Alternative hypothesis “ x is greater than y .”
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13 Different Types of Hypothesis

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There are 13 different types of hypothesis. These include simple, complex, null, alternative, composite, directional, non-directional, logical, empirical, statistical, associative, exact, and inexact.

A hypothesis can be categorized into one or more of these types. However, some are mutually exclusive and opposites. Simple and complex hypotheses are mutually exclusive, as are direction and non-direction, and null and alternative hypotheses.

Below I explain each hypothesis in simple terms for absolute beginners. These definitions may be too simple for some, but they’re designed to be clear introductions to the terms to help people wrap their heads around the concepts early on in their education about research methods .

Types of Hypothesis

Before you Proceed: Dependent vs Independent Variables

A research study and its hypotheses generally examine the relationships between independent and dependent variables – so you need to know these two concepts:

• The independent variable is the variable that is causing a change.
• The dependent variable is the variable the is affected by the change. This is the variable being tested.

Read my full article on dependent vs independent variables for more examples.

Example: Eating carrots (independent variable) improves eyesight (dependent variable).

1. Simple Hypothesis

A simple hypothesis is a hypothesis that predicts a correlation between two test variables: an independent and a dependent variable.

This is the easiest and most straightforward type of hypothesis. You simply need to state an expected correlation between the dependant variable and the independent variable.

You do not need to predict causation (see: directional hypothesis). All you would need to do is prove that the two variables are linked.

Simple Hypothesis Examples

2. Complex Hypothesis

A complex hypothesis is a hypothesis that contains multiple variables, making the hypothesis more specific but also harder to prove.

You can have multiple independent and dependant variables in this hypothesis.

Complex Hypothesis Example

In the above example, we have multiple independent and dependent variables:

• Independent variables: Age and weight.
• Dependent variables: diabetes and heart disease.

Because there are multiple variables, this study is a lot more complex than a simple hypothesis. It quickly gets much more difficult to prove these hypotheses. This is why undergraduate and first-time researchers are usually encouraged to use simple hypotheses.

3. Null Hypothesis

A null hypothesis will predict that there will be no significant relationship between the two test variables.

For example, you can say that “The study will show that there is no correlation between marriage and happiness.”

A good way to think about a null hypothesis is to think of it in the same way as “innocent until proven guilty”[1]. Unless you can come up with evidence otherwise, your null hypothesis will stand.

A null hypothesis may also highlight that a correlation will be inconclusive . This means that you can predict that the study will not be able to confirm your results one way or the other. For example, you can say “It is predicted that the study will be unable to confirm a correlation between the two variables due to foreseeable interference by a third variable .”

Beware that an inconclusive null hypothesis may be questioned by your teacher. Why would you conduct a test that you predict will not provide a clear result? Perhaps you should take a closer look at your methodology and re-examine it. Nevertheless, inconclusive null hypotheses can sometimes have merit.

Null Hypothesis Examples

4. Alternative Hypothesis

An alternative hypothesis is a hypothesis that is anything other than the null hypothesis. It will disprove the null hypothesis.

We use the symbol H A or H 1 to denote an alternative hypothesis.

The null and alternative hypotheses are usually used together. We will say the null hypothesis is the case where a relationship between two variables is non-existent. The alternative hypothesis is the case where there is a relationship between those two variables.

The following statement is always true: H 0 ≠ H A .

Let’s take the example of the hypothesis: “Does eating oatmeal before an exam impact test scores?”

We can have two hypotheses here:

• Null hypothesis (H 0 ): “Eating oatmeal before an exam does not impact test scores.”
• Alternative hypothesis (H A ): “Eating oatmeal before an exam does impact test scores.”

For the alternative hypothesis to be true, all we have to do is disprove the null hypothesis for the alternative hypothesis to be true. We do not need an exact prediction of how much oatmeal will impact the test scores or even if the impact is positive or negative. So long as the null hypothesis is proven to be false, then the alternative hypothesis is proven to be true.

5. Composite Hypothesis

A composite hypothesis is a hypothesis that does not predict the exact parameters, distribution, or range of the dependent variable.

Often, we would predict an exact outcome. For example: “23 year old men are on average 189cm tall.” Here, we are giving an exact parameter. So, the hypothesis is not composite.

But, often, we cannot exactly hypothesize something. We assume that something will happen, but we’re not exactly sure what. In these cases, we might say: “23 year old men are not on average 189cm tall.”

We haven’t set a distribution range or exact parameters of the average height of 23 year old men. So, we’ve introduced a composite hypothesis as opposed to an exact hypothesis.

Generally, an alternative hypothesis (discussed above) is composite because it is defined as anything except the null hypothesis. This ‘anything except’ does not define parameters or distribution, and therefore it’s an example of a composite hypothesis.

6. Directional Hypothesis

A directional hypothesis makes a prediction about the positivity or negativity of the effect of an intervention prior to the test being conducted.

Instead of being agnostic about whether the effect will be positive or negative, it nominates the effect’s directionality.

We often call this a one-tailed hypothesis (in contrast to a two-tailed or non-directional hypothesis) because, looking at a distribution graph, we’re hypothesizing that the results will lean toward one particular tail on the graph – either the positive or negative.

Directional Hypothesis Examples

7. Non-Directional Hypothesis

A non-directional hypothesis does not specify the predicted direction (e.g. positivity or negativity) of the effect of the independent variable on the dependent variable.

These hypotheses predict an effect, but stop short of saying what that effect will be.

A non-directional hypothesis is similar to composite and alternative hypotheses. All three types of hypothesis tend to make predictions without defining a direction. In a composite hypothesis, a specific prediction is not made (although a general direction may be indicated, so the overlap is not complete). For an alternative hypothesis, you often predict that the even will be anything but the null hypothesis, which means it could be more or less than H 0 (or in other words, non-directional).

Let’s turn the above directional hypotheses into non-directional hypotheses.

Non-Directional Hypothesis Examples

8. Logical Hypothesis

A logical hypothesis is a hypothesis that cannot be tested, but has some logical basis underpinning our assumptions.

These are most commonly used in philosophy because philosophical questions are often untestable and therefore we must rely on our logic to formulate logical theories.

Usually, we would want to turn a logical hypothesis into an empirical one through testing if we got the chance. Unfortunately, we don’t always have this opportunity because the test is too complex, expensive, or simply unrealistic.

Here are some examples:

• Before the 1980s, it was hypothesized that the Titanic came to its resting place at 41° N and 49° W, based on the time the ship sank and the ship’s presumed path across the Atlantic Ocean. However, due to the depth of the ocean, it was impossible to test. Thus, the hypothesis was simply a logical hypothesis.
• Dinosaurs closely related to Aligators probably had green scales because Aligators have green scales. However, as they are all extinct, we can only rely on logic and not empirical data.

9. Empirical Hypothesis

An empirical hypothesis is the opposite of a logical hypothesis. It is a hypothesis that is currently being tested using scientific analysis. We can also call this a ‘working hypothesis’.

We can to separate research into two types: theoretical and empirical. Theoretical research relies on logic and thought experiments. Empirical research relies on tests that can be verified by observation and measurement.

So, an empirical hypothesis is a hypothesis that can and will be tested.

• Raising the wage of restaurant servers increases staff retention.
• Adding 1 lb of corn per day to cows’ diets decreases their lifespan.
• Mushrooms grow faster at 22 degrees Celsius than 27 degrees Celsius.

Each of the above hypotheses can be tested, making them empirical rather than just logical (aka theoretical).

10. Statistical Hypothesis

A statistical hypothesis utilizes representative statistical models to draw conclusions about broader populations.

It requires the use of datasets or carefully selected representative samples so that statistical inference can be drawn across a larger dataset.

This type of research is necessary when it is impossible to assess every single possible case. Imagine, for example, if you wanted to determine if men are taller than women. You would be unable to measure the height of every man and woman on the planet. But, by conducting sufficient random samples, you would be able to predict with high probability that the results of your study would remain stable across the whole population.

You would be right in guessing that almost all quantitative research studies conducted in academic settings today involve statistical hypotheses.

Statistical Hypothesis Examples

• Human Sex Ratio. The most famous statistical hypothesis example is that of John Arbuthnot’s sex at birth case study in 1710. Arbuthnot used birth data to determine with high statistical probability that there are more male births than female births. He called this divine providence, and to this day, his findings remain true: more men are born than women.
• Lady Testing Tea. A 1935 study by Ronald Fisher involved testing a woman who believed she could tell whether milk was added before or after water to a cup of tea. Fisher gave her 4 cups in which one randomly had milk placed before the tea. He repeated the test 8 times. The lady was correct each time. Fisher found that she had a 1 in 70 chance of getting all 8 test correct, which is a statistically significant result.

11. Associative Hypothesis

An associative hypothesis predicts that two variables are linked but does not explore whether one variable directly impacts upon the other variable.

We commonly refer to this as “ correlation does not mean causation ”. Just because there are a lot of sick people in a hospital, it doesn’t mean that the hospital made the people sick. There is something going on there that’s causing the issue (sick people are flocking to the hospital).

So, in an associative hypothesis, you note correlation between an independent and dependent variable but do not make a prediction about how the two interact. You stop short of saying one thing causes another thing.

Associative Hypothesis Examples

• Sick people in hospital. You could conduct a study hypothesizing that hospitals have more sick people in them than other institutions in society. However, you don’t hypothesize that the hospitals caused the sickness.
• Lice make you healthy. In the Middle Ages, it was observed that sick people didn’t tend to have lice in their hair. The inaccurate conclusion was that lice was not only a sign of health, but that they made people healthy. In reality, there was an association here, but not causation. The fact was that lice were sensitive to body temperature and fled bodies that had fevers.

12. Causal Hypothesis

A causal hypothesis predicts that two variables are not only associated, but that changes in one variable will cause changes in another.

A causal hypothesis is harder to prove than an associative hypothesis because the cause needs to be definitively proven. This will often require repeating tests in controlled environments with the researchers making manipulations to the independent variable, or the use of control groups and placebo effects .

If we were to take the above example of lice in the hair of sick people, researchers would have to put lice in sick people’s hair and see if it made those people healthier. Researchers would likely observe that the lice would flee the hair, but the sickness would remain, leading to a finding of association but not causation.

Causal Hypothesis Examples

13. Exact vs. Inexact Hypothesis

For brevity’s sake, I have paired these two hypotheses into the one point. The reality is that we’ve already seen both of these types of hypotheses at play already.

An exact hypothesis (also known as a point hypothesis) specifies a specific prediction whereas an inexact hypothesis assumes a range of possible values without giving an exact outcome. As Helwig [2] argues:

“An “exact” hypothesis specifies the exact value(s) of the parameter(s) of interest, whereas an “inexact” hypothesis specifies a range of possible values for the parameter(s) of interest.”

Generally, a null hypothesis is an exact hypothesis whereas alternative, composite, directional, and non-directional hypotheses are all inexact.

See Next: 15 Hypothesis Examples

This is introductory information that is basic and indeed quite simplified for absolute beginners. It’s worth doing further independent research to get deeper knowledge of research methods and how to conduct an effective research study. And if you’re in education studies, don’t miss out on my list of the best education studies dissertation ideas .

[1] https://jnnp.bmj.com/content/91/6/571.abstract

[2] http://users.stat.umn.edu/~helwig/notes/SignificanceTesting.pdf

• Chris Drew (PhD) https://helpfulprofessor.com/author/chris-drew-phd/ 101 Class Group Name Ideas (for School Students)
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2 thoughts on “13 Different Types of Hypothesis”

Wow! This introductionary materials are very helpful. I teach the begginers in research for the first time in my career. The given tips and materials are very helpful. Chris, thank you so much! Excellent materials!

You’re more than welcome! If you want a pdf version of this article to provide for your students to use as a weekly reading on in-class discussion prompt for seminars, just drop me an email in the Contact form and I’ll get one sent out to you.

When I’ve taught this seminar, I’ve put my students into groups, cut these definitions into strips, and handed them out to the groups. Then I get them to try to come up with hypotheses that fit into each ‘type’. You can either just rotate hypothesis types so they get a chance at creating a hypothesis of each type, or get them to “teach” their hypothesis type and examples to the class at the end of the seminar.

Cheers, Chris

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Once you have developed a clear and focused research question or set of research questions, you’ll be ready to conduct further research, a literature review, on the topic to help you make an educated guess about the answer to your question(s). This educated guess is called a hypothesis.

In research, there are two types of hypotheses: null and alternative. They work as a complementary pair, each stating that the other is wrong.

• Null Hypothesis (H 0 ) – This can be thought of as the implied hypothesis. “Null” meaning “nothing.”  This hypothesis states that there is no difference between groups or no relationship between variables. The null hypothesis is a presumption of status quo or no change.
• Alternative Hypothesis (H a ) – This is also known as the claim. This hypothesis should state what you expect the data to show, based on your research on the topic. This is your answer to your research question.

Null Hypothesis:   H 0 : There is no difference in the salary of factory workers based on gender. Alternative Hypothesis :  H a : Male factory workers have a higher salary than female factory workers.

Null Hypothesis :  H 0 : There is no relationship between height and shoe size. Alternative Hypothesis :  H a : There is a positive relationship between height and shoe size.

Null Hypothesis :  H 0 : Experience on the job has no impact on the quality of a brick mason’s work. Alternative Hypothesis :  H a : The quality of a brick mason’s work is influenced by on-the-job experience.

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Null hypothesis and alternative hypothesis with 9 differences

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Null hypothesis definition

The null hypothesis is a general statement that states that there is no relationship between two phenomenons under consideration or that there is no association between two groups.

• A hypothesis, in general, is an assumption that is yet to be proved with sufficient pieces of evidence. A null hypothesis thus is the hypothesis a researcher is trying to disprove.
• A null hypothesis is a hypothesis capable of being objectively verified, tested, and even rejected.
• If a study is to compare method A with method B about their relationship, and if the study is preceded on the assumption that both methods are equally good, then this assumption is termed as the null hypothesis.
• The null hypothesis should always be a specific hypothesis, i.e., it should not state about or approximately a certain value.

Null hypothesis symbol

• The symbol for the null hypothesis is H 0, and it is read as H-null, H-zero, or H-naught.
• The null hypothesis is usually associated with just ‘equals to’ sign as a null hypothesis can either be accepted or rejected.

Null hypothesis purpose

• The main purpose of a null hypothesis is to verify/ disprove the proposed statistical assumptions.
• Some scientific null hypothesis help to advance a theory.
• The null hypothesis is also used to verify the consistent results of multiple experiments. For e.g., the null hypothesis stating that there is no relation between some medication and age of the patients supports the general effectiveness conclusion, and allows recommendations.

Null hypothesis principle

• The principle of the null hypothesis is collecting the data and determining the chances of the collected data in the study of a random sample, proving that the null hypothesis is true.
• In situations or studies where the collected data doesn’t complete the expectation of the null hypothesis, it is concluded that the data doesn’t provide sufficient or reliable pieces of evidence to support the null hypothesis and thus, it is rejected.
• The data collected is tested through some statistical tool which is designed to measure the extent of departure of the date from the null hypothesis.
• The procedure decides whether the observed departure obtained from the statistical tool is larger than a defined value so that the probability of occurrence of a high departure value is very small under the null hypothesis.
• However, some data might not contradict the null hypothesis which explains that only a weak conclusion can be made and that the data doesn’t provide strong pieces of evidence against the null hypothesis and the null hypothesis might or might not be true.
• Under some other conditions, if the data collected is sufficient and is capable of providing enough evidence, the null hypothesis can be considered valid, indicating no relationship between the phenomena.

When to reject null hypothesis?

• When the p-value of the data is less than the significant level of the test, the null hypothesis is rejected, indicating the test results are significant.
• However, if the p-value is higher than the significant value, the null hypothesis is not rejected, and the results are considered not significant.
• The level of significance is an important concept while hypothesis testing as it determines the percentage risk of rejecting the null hypothesis when H 0 might happen to be true.
• In other words, if we take the level of significance at 5%, it means that the researcher is willing to take as much as a 5 percent risk of rejecting the null hypothesis when it (H 0 ) happens to be true.
• The null hypothesis cannot be accepted because the lack of evidence only means that the relationship is not proven. It doesn’t prove that something doesn’t exist, but it just means that there are not enough shreds of evidence and the study might have missed it.

Null hypothesis examples

The following are some examples of null hypothesis:

• If the hypothesis is that “the consumption of a particular medicine reduces the chances of heart arrest”, the null hypothesis will be “the consumption of the medicine doesn’t reduce the chances of heart arrest.”
• If the hypothesis is that, “If random test scores are collected from men and women, does the score of one group differ from the other?” a possible null hypothesis will be that the mean test score of men is the same as that of the women.

H 0 : µ 1 = µ 2

H 0 = null hypothesis µ 1 = mean score of men µ 2 = mean score of women

Alternative hypothesis definition

An alternative hypothesis is a statement that describes that there is a relationship between two selected variables in a study.

• An alternative hypothesis is usually used to state that a new theory is preferable to the old one (null hypothesis).
• This hypothesis can be simply termed as an alternative to the null hypothesis.
• The alternative hypothesis is the hypothesis that is to be proved that indicates that the results of a study are significant and that the sample observation is not results just from chance but from some non-random cause.
• If a study is to compare method A with method B about their relationship and we assume that the method A is superior or the method B is inferior, then such a statement is termed as an alternative hypothesis.
• Alternative hypotheses should be clearly stated, considering the nature of the research problem.

Alternative hypothesis symbol

• The symbol of the alternative hypothesis is either H 1 or H a while using less than, greater than or not equal signs.

Alternative hypothesis purpose

• An alternative hypothesis provides the researchers with some specific restatements and clarifications of the research problem.
• An alternative hypothesis provides a direction to the study, which then can be utilized by the researcher to obtain the desired results.
• Since the alternative hypothesis is selected before conducting the study, it allows the test to prove that the study is supported by evidence, separating it from the researchers’ desires and values.
• An alternative hypothesis provides a chance of discovering new theories that can disprove an existing one that might not be supported by evidence.
• The alternative hypothesis is important as they prove that a relationship exists between two variables selected and that the results of the study conducted are relevant and significant.

Alternative hypothesis principle

• The principle behind the alternative hypothesis is similar to that of the null hypothesis.
• The alternative hypothesis is based on the concept that when sufficient evidence is collected from the data of random sample, it provides a basis for proving the assumption made by the researcher regarding the study.
• Like in the null hypothesis, the data collected from a random sample is passed through a statistical tool that measures the extent of departure of the data from the null hypothesis.
• If the departure is small under the selected level of significance, the alternative hypothesis is accepted, and the null hypothesis is rejected.
• If the data collected don’t have chances of being in the study of the random sample and are instead decided by the relationship within the sample of the study, an alternative hypothesis stands true.

Alternative hypothesis examples

The following are some examples of alternative hypothesis:

1. If a researcher is assuming that the bearing capacity of a bridge is more than 10 tons, then the hypothesis under this study will be:

Null hypothesis H 0 : µ= 10 tons Alternative hypothesis H a : µ>10 tons

2. Under another study that is trying to test whether there is a significant difference between the effectiveness of medicine against heart arrest, the alternative hypothesis will be that there is a relationship between the medicine and chances of heart arrest.

Null hypothesis vs Alternative hypothesis

• R. Kothari (1990) Research Methodology. Vishwa Prakasan. India.
• https://www.statisticssolutions.com/null-hypothesis-and-alternative-hypothesis/
• https://byjus.com/maths/null-hypothesis/
• https://en.wikipedia.org/wiki/Null_hypothesis
• https://keydifferences.com/difference-between-null-and-alternative-hypothesis.html
• 5% – https://en.wikipedia.org/wiki/Null_hypothesis
• 3% – https://keydifferences.com/difference-between-null-and-alternative-hypothesis.html
• 2% – https://byjus.com/maths/null-hypothesis/
• 1% – https://www.wisdomjobs.com/e-university/research-methodology-tutorial-355/procedure-for-hypothesis-testing-11525.html
• 1% – https://www.thoughtco.com/definition-of-null-hypothesis-and-examples-605436
• 1% – https://www.quora.com/What-are-the-different-types-of-hypothesis-and-what-are-some-examples-of-them
• 1% – https://www.dummies.com/education/math/statistics/what-a-p-value-tells-you-about-statistical-data/
• 1% – https://www.coursehero.com/file/p7jfbal5/These-are-hypotheses-capable-of-being-objectively-verified-and-tested-Thus-we/
• 1% – https://support.minitab.com/en-us/minitab/18/help-and-how-to/modeling-statistics/anova/how-to/one-way-anova/interpret-the-results/all-statistics-and-graphs/methods/
• 1% – https://stats.stackexchange.com/questions/105319/test-whether-there-is-a-significant-difference-between-two-groups
• 1% – https://statisticsbyjim.com/hypothesis-testing/failing-reject-null-hypothesis/
• 1% – https://quizlet.com/45299306/statistics-flash-cards/
• <1% – https://www.thoughtco.com/significance-level-in-hypothesis-testing-1147177
• <1% – https://www.thoughtco.com/null-hypothesis-vs-alternative-hypothesis-3126413
• <1% – https://www.sagepub.com/sites/default/files/upm-binaries/40007_Chapter8.pdf
• <1% – https://www.differencebetween.com/difference-between-hypothesis-and-vs-assumption/
• <1% – https://www.coursehero.com/file/18076181/introduction-to-hypothesis/
• <1% – https://statisticsbyjim.com/glossary/significance-level/
• <1% – https://quizlet.com/164755799/research-methods-midterm-2-flash-cards/
• <1% – https://online.stat.psu.edu/statprogram/reviews/statistical-concepts/hypothesis-testing/p-value-approach

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Null hypothesis and Alternative Hypothesis

A hypothesis is a proposed explanation for a phenomenon, based on observation, reasoning, or scientific theory, awaiting verification or falsification through experimentation and data analysis. It serves as a starting point for investigation, guiding the research process by suggesting what outcomes to expect. In the realm of statistics and scientific research, hypotheses are crucial for designing experiments, analyzing results, and advancing knowledge.

The null hypothesis and alternative hypothesis are required to be fragmented properly before the data collection and interpretation phase in the research. Well fragmented hypotheses indicate that the researcher has adequate knowledge in that particular area and is thus able to take the investigation further because they can use a much more systematic system. It gives direction to the researcher on his/her collection and interpretation of data.

The null hypothesis and alternative hypothesis are useful only if they state the expected relationship between the variables or if they are consistent with the existing body of knowledge. They should be expressed as simply and concisely as possible. They are useful if they have explanatory power.

The purpose and importance of the null hypothesis and alternative hypothesis are that they provide an approximate description of the phenomena. The purpose is to provide the researcher or an investigator with a relational statement that is directly tested in a research study. The purpose is to provide the framework for reporting the inferences of the study. The purpose is to behave as a working instrument of the theory. The purpose is to prove whether or not the test is supported, which is separated from the investigator’s own values and decisions. They also provide direction to the research.

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The null hypothesis is generally denoted as H0. It states the exact opposite of what an investigator or an experimenter predicts or expects. It basically defines the statement which states that there is no exact or actual relationship between the variables.

The alternative hypothesis is generally denoted as H1. It makes a statement that suggests or advises a potential result or an outcome that an investigator or the researcher may expect. It has been categorized into two categories: directional alternative hypothesis and non directional alternative hypothesis.

The directional hypothesis is a kind that explains the direction of the expected findings. Sometimes this type of alternative hypothesis is developed to examine the relationship among the variables rather than a comparison between the groups.

The non directional hypothesis is a kind that has no definite direction of the expected findings being specified.

Related Pages:

Hypothesis Testing

Research Hypotheses

Ready to test your hypothesis? Check out Intellectus Statistics , the easy to use statistics software for the non-statistician.

• Alternative vs Null Hypothesis: Pros, Cons, Uses & Examples

All research starts with a problem that needs to be solved. From this problem, hypotheses are developed to provide the researcher with a clear statement of the problem.

To understand alternative hypotheses also known as alternate hypotheses, you must first understand what the hypothesis is .

When you hear the word hypothesis it means the accurate explanations in relation to a set of facts that can be analyzed when studied, using some specific method of research.

There are primarily two types of hypothesis which are null hypothesis and alternative hypothesis.

When you think about the word “null” what should come to mind is something that can not change, what you expect is what you get, unlike alternate hypotheses which can change.

Now, the research problems or questions which could be in the form of null hypothesis or alternative hypothesis are expressed as the relationship that exists between two or more variables. The process for this states that the questions should be what expresses the relationship between two variables that can be measured.

Both null hypotheses and alternative hypotheses are used by statisticians and researchers to conduct research in various industries or fields such as mathematics, psychology, science, medicine, and technology.

We are going to discuss alternative hypotheses and null hypotheses in this post and how they work in research.

What is an Alternative Hypothesis?

Alternative hypothesis simply put is another viable option to the null hypothesis. It means looking for a substantial change or option that can allow you to reject the null hypothesis.

It is an opposing theory to a null hypothesis.

If you develop a null hypothesis, you make an informed guess on whether a thing is true or whether there is a relationship between that thing and another variable. An alternate hypothesis will always take an opposite stand against a null hypothesis. So if according to a null hypothesis something is correct to an alternate hypothesis that same thing will be incorrect.

For example, let’s assume that you develop a null hypothesis that states “I”m going to be $500 richer” the alternate hypothesis will be “I’m going to get $500 or be richer”

When you are trying to disprove a null hypothesis, that is when you test an alternate hypothesis. If there is enough data to back up the alternative hypothesis then you can dispose of the null hypothesis. 

Get Answers: What is Empirical Research Study? [Examples & Method]

What is a Null Hypothesis?

The null hypothesis is best explained as the statement showing that no relationship exists between two variables that are being considered or that two groups are not related. As we have earlier established, a hypothesis is an assumed statement that has not been proven with sufficient data that could serve as a piece of evidence. 

The null hypothesis is now the statement that a researcher or an investigator wants to disprove. The null hypothesis is capable of being tested, being verifiable, and also capable of being rejected.

For example, if you want to conduct a study that will compare the relationship between project A and project B if the study is based on the assumption that both projects are of equal standard, the assumption is referred to as the null hypothesis.

This is because the null hypothesis should be specific at all times.

Learn: Hypothesis Testing in Research: Definition, Procedure, Uses, Limitations + Examples

Advantages of the Alternative Hypothesis 

• Alternative hypothesis gives a researcher specific clarifications on the research questions or problems .
• It provides a study with the direction that can be used to collect data and obtain results of interest by the researcher.
• An alternative hypothesis is always selected before commencing the studies which gives the researcher the opportunity to prove that the restatement is backed up by evidence and not just from the researcher’s ideas or values.
• Another good thing about alternative hypotheses is that it provides the opportunity to discover new theories that a researcher can use to disprove an existing theory that may not have been backed up by evidence .
• An alternate hypothesis is also useful to prove that there is a relationship between two selected variables and the outcomes of the conducted study are relevant.

Principles of the Alternative Hypothesis

• Alternative hypotheses will be accepted if the amount of data that is gone is insignificant within the significance level. This means that the null hypothesis will be rejected.
• Another principle of the alternative hypothesis is that the data gathered from random samples go through a statistical tool that analyzes the effect of the amount of data leaving the null hypothesis.

For the curious: Sampling Bias: Definition, Types + [Examples]

Purpose of the Null Hypothesis 

Here are the purposes of the null hypothesis in an experiment or study:

• The primary purpose of a null hypothesis is to disprove an assumption.
• Null hypotheses can help to further progress a theory in some scientific cases.
• You can also use a null hypothesis to ascertain how consistent the outcomes of multiple studies are.

Principle of the Null Hypothesis 

Now, these are the principles of the null hypothesis:

1. The primary principle of the null hypothesis is to prove that the assumed statement is true. This is done by collecting data and analyzing in the study , what chance the collected data has in the random sample.

2. If the collected data does not meet the expectation of the null hypothesis, it is determined that the data lacks sufficient evidence to back up the null hypothesis therefore the null hypothesis statement is rejected.

Just as in the case of the alternative hypothesis the collected data in a null hypothesis is analyzed using some statistical tools that are made to measure the extent to which data left the null hypothesis.

The process will determine whether the data that left the null hypothesis is larger than a set value. If the data collected from the random sample is enough to serve as evidence to prove the null hypothesis then the null hypothesis will be accepted as true. And also defined that it has no relationship with other variables .

Learn About: Research Reports: Definition, Types + [Writing Guide]

Types of Alternative Hypothesis (Advantages of Each and When to Use)

There are four types of alternative hypotheses, and we will briefly discuss them below.

• One-tailed directional: For one of the sampling distributions one tail, this type of alternative hypothesis focuses on the rejected part only.
• Two-tailed directional: In an alternative hypothesis, a two-tailed directional focus on the two parts or directions that were rejected in the sampling distribution.
• Point: Point is another alternate hypothesis. It occurs in hypothesis testing when the sample population in the alternate hypothesis has been completely defined in a distribution. If there are no known parameters, the hypothesis will serve no interest. They are, however, important to the foundation of the statistical inferences.
• Non-directional: In an alternate hypothesis, a non-directional does not focus on the two directions of rejection. The only focus of the nondirectional alternative hypothesis is to prove that the null hypothesis is incorrect.

Read: Type I vs Type II Errors: Definition, Examples & Prevention

Difference between Null Hypothesis and Alternative Hypothesis 

We are going to look at the differences between the alternate hypothesis and the null hypothesis based on these six factors which are:

• Mathematical expression
• Observation
• Acceptance criteria
• The difference in Mathematical expression

Null hypothesis is followed by an ‘equals to’ (=) sign. While the Alternative hypothesis is followed by these three signs; 

• The difference in how they are observed

In the null hypothesis, it is believed that the results that are observed are as a result of chance. While In the alternative hypothesis, it is believed that the observed results are the outcome of some real causes.

• Differences in results

The result of the null hypothesis always shows that there have been no changes in statements or opinions. While the result of the alternative hypothesis shows that there have been significant changes in statements and opinions.

• Differences in Acceptance criteria

If the p-value in a null hypothesis is greater than the significance level, then the null hypothesis is accepted.

If the p-value in an alternate hypothesis is smaller than the significance level, then the alternative hypothesis is accepted.

• Differences in importance

The null hypothesis accepts true existing theories and also if there has been consistency in multiple experiments of similar hypotheses.

The alternative hypothesis establishes whether a relationship exists between two variables, and the result will then lead to new improved theories.

Read: T-testing: Definition, Formula & Interpretations

Examples of an Alternative Hypothesis and Null Hypothesis

Here are some examples of the alternative hypothesis:.

A researcher assumes that a bridge’s bearing capacity is over 10 tons, the researcher will then develop an hypothesis to support this study. The hypothesis will be:

For the null hypothesis H0: µ= 10 tons

For the alternate hypothesis Ha: µ>10 tons

In another study being conducted, the researcher wants to find out whether there is a noticeable difference or change in a patient’s heart arrest medicine and the patient’s heart condition.

For the alternate hypothesis: The hypothesis is that there might indeed be a relationship between the new medicine and the frequency or chances of heart arrest in a patient.

Here are the examples of the null hypothesis

The hypothesis from example 2 in the alternate hypothesis implies that the use of one specific medicine can reduce the frequency and chances of heart arrest.

For the null hypothesis: The hypothesis will be that the use of that particular medicine cannot reduce the chance and frequency of heart arrest in a patient.

An alternate hypothesis states that the random exam scores are collected from both men and women. But are the scores of the two groups (men and women) the same or are they different?

For the null hypothesis: The hypothesis will state that the calculated mean of the men’s exam score is equal to the exam score of the women.

This is represented as

H0= The null hypothesis

µ1= The calculated mean score of men

µ2= The calculated mean score of women

Read: What is Empirical Research Study? [Examples & Method]

• Can you reject an alternative hypothesis?

It is quite inappropriate to say or report that an alternate hypothesis was rejected. It is much better to use the phrase “the alternate hypothesis was rather not supported”.

The reason behind this use of words is that only the null hypothesis is designed to be rejected in a study. The alternative hypothesis is designed to prove the null hypothesis incorrect, to introduce new facts that can disprove the null hypothesis but it is not designed to be rejected.

It can either be accepted or not supported.

• How do you identify alternative hypotheses?

A researcher can use this formula to identify the alternate hypothesis in a study or experiment.

H0 and Ha are in contrast.

Therefore, if Ho has:

Equal to (=)

Greater than or equal to (≥)

Less than or equal to (≤)

And then Ha has:

Not equal (≠) 

Greater than (>) or less than (

Less than ( )

If in a study, α ≤ p-value, then the researcher should not reject H0.

If in a study, α > p-value, then the researcher should reject H0.

α is preconceived. The value of α is determined even before the hypothesis test is conducted. While the p-value is derived from the calculation in the data.

• Which is better in formulating hypotheses of your study alternative or null?

The study a researcher wants to conduct will determine what hypothesis should be developed. However, the researcher should keep in mind what the purpose of the null and alternative two hypotheses are while developing the study hypothesis. So while the null hypothesis will accept existing theories that it found to be true or correct, and measure the consistency of multiple experiments, alternative hypotheses will find the relationship that exists (if any) between two phenomena and may lead to the development of a new and improved theory.

In this article, it has been clearly defined the relationship that exists between the null hypothesis and the alternative hypothesis. While the null hypothesis is always an assumption that needs to be proven with evidence for it to be accepted, the alternative hypothesis puts in all the effort to make sure the null hypothesis is disproved. 

Researchers should note that for every null hypothesis, one or more alternate hypotheses can be developed.

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• Key Differences

Know the Differences & Comparisons

Difference Between Null and Alternative Hypothesis

Null hypothesis implies a statement that expects no difference or effect. On the contrary, an alternative hypothesis is one that expects some difference or effect. Null hypothesis This article excerpt shed light on the fundamental differences between null and alternative hypothesis.

Content: Null Hypothesis Vs Alternative Hypothesis

Comparison chart.

Definition of Null Hypothesis

A null hypothesis is a statistical hypothesis in which there is no significant difference exist between the set of variables. It is the original or default statement, with no effect, often represented by H 0 (H-zero). It is always the hypothesis that is tested. It denotes the certain value of population parameter such as µ, s, p. A null hypothesis can be rejected, but it cannot be accepted just on the basis of a single test.

Definition of Alternative Hypothesis

A statistical hypothesis used in hypothesis testing, which states that there is a significant difference between the set of variables. It is often referred to as the hypothesis other than the null hypothesis, often denoted by H 1 (H-one). It is what the researcher seeks to prove in an indirect way, by using the test. It refers to a certain value of sample statistic, e.g., x¯, s, p

The acceptance of alternative hypothesis depends on the rejection of the null hypothesis i.e. until and unless null hypothesis is rejected, an alternative hypothesis cannot be accepted.

Key Differences Between Null and Alternative Hypothesis

The important points of differences between null and alternative hypothesis are explained as under:

• A null hypothesis is a statement, in which there is no relationship between two variables. An alternative hypothesis is a statement; that is simply the inverse of the null hypothesis, i.e. there is some statistical significance between two measured phenomenon.
• A null hypothesis is what, the researcher tries to disprove whereas an alternative hypothesis is what the researcher wants to prove.
• A null hypothesis represents, no observed effect whereas an alternative hypothesis reflects, some observed effect.
• If the null hypothesis is accepted, no changes will be made in the opinions or actions. Conversely, if the alternative hypothesis is accepted, it will result in the changes in the opinions or actions.
• As null hypothesis refers to population parameter, the testing is indirect and implicit. On the other hand, the alternative hypothesis indicates sample statistic, wherein, the testing is direct and explicit.
• A null hypothesis is labelled as H 0 (H-zero) while an alternative hypothesis is represented by H 1 (H-one).
• The mathematical formulation of a null hypothesis is an equal sign but for an alternative hypothesis is not equal to sign.
• In null hypothesis, the observations are the outcome of chance whereas, in the case of the alternative hypothesis, the observations are an outcome of real effect.

There are two outcomes of a statistical test, i.e. first, a null hypothesis is rejected and alternative hypothesis is accepted, second, null hypothesis is accepted, on the basis of the evidence. In simple terms, a null hypothesis is just opposite of alternative hypothesis.

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Zipporah Thuo says

February 22, 2018 at 6:06 pm

The comparisons between the two hypothesis i.e Null hypothesis and the Alternative hypothesis are the best.Thank you.

Getu Gamo says

March 4, 2019 at 3:42 am

Thank you so much for the detail explanation on two hypotheses. Now I understood both very well, including their differences.

Jyoti Bhardwaj says

May 28, 2019 at 6:26 am

Thanks, Surbhi! Appreciate the clarity and precision of this content.

January 9, 2020 at 6:16 am

John Jenstad says

July 20, 2020 at 2:52 am

Thanks very much, Surbhi, for your clear explanation!!

Navita says

July 2, 2021 at 11:48 am

Thanks for the Comparison chart! it clears much of my doubt.

GURU UPPALA says

July 21, 2022 at 8:36 pm

Thanks for the Comparison chart!

Enock kipkoech says

September 22, 2022 at 1:57 pm

What are the examples of null hypothesis and substantive hypothesis

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• Math Article

Null Hypothesis

In mathematics, Statistics deals with the study of research and surveys on the numerical data. For taking surveys, we have to define the hypothesis. Generally, there are two types of hypothesis. One is a null hypothesis, and another is an alternative hypothesis .

In probability and statistics, the null hypothesis is a comprehensive statement or default status that there is zero happening or nothing happening. For example, there is no connection among groups or no association between two measured events. It is generally assumed here that the hypothesis is true until any other proof has been brought into the light to deny the hypothesis. Let us learn more here with definition, symbol, principle, types and example, in this article.

Table of contents:

• Comparison with Alternative Hypothesis

Null Hypothesis Definition

The null hypothesis is a kind of hypothesis which explains the population parameter whose purpose is to test the validity of the given experimental data. This hypothesis is either rejected or not rejected based on the viability of the given population or sample . In other words, the null hypothesis is a hypothesis in which the sample observations results from the chance. It is said to be a statement in which the surveyors wants to examine the data. It is denoted by H 0 .

Null Hypothesis Symbol

In statistics, the null hypothesis is usually denoted by letter H with subscript ‘0’ (zero), such that H 0 . It is pronounced as H-null or H-zero or H-nought. At the same time, the alternative hypothesis expresses the observations determined by the non-random cause. It is represented by H 1 or H a .

Null Hypothesis Principle

The principle followed for null hypothesis testing is, collecting the data and determining the chances of a given set of data during the study on some random sample, assuming that the null hypothesis is true. In case if the given data does not face the expected null hypothesis, then the outcome will be quite weaker, and they conclude by saying that the given set of data does not provide strong evidence against the null hypothesis because of insufficient evidence. Finally, the researchers tend to reject that.

Null Hypothesis Formula

Here, the hypothesis test formulas are given below for reference.

The formula for the null hypothesis is:

H 0 :  p = p 0

The formula for the alternative hypothesis is:

H a = p >p 0 , < p 0 ≠ p 0

The formula for the test static is:

Remember that,  p 0  is the null hypothesis and p – hat is the sample proportion.

Also, read:

Types of Null Hypothesis

There are different types of hypothesis. They are:

Simple Hypothesis

It completely specifies the population distribution. In this method, the sampling distribution is the function of the sample size.

Composite Hypothesis

The composite hypothesis is one that does not completely specify the population distribution.

Exact Hypothesis

Exact hypothesis defines the exact value of the parameter. For example μ= 50

Inexact Hypothesis

This type of hypothesis does not define the exact value of the parameter. But it denotes a specific range or interval. For example 45< μ <60

Null Hypothesis Rejection

Sometimes the null hypothesis is rejected too. If this hypothesis is rejected means, that research could be invalid. Many researchers will neglect this hypothesis as it is merely opposite to the alternate hypothesis. It is a better practice to create a hypothesis and test it. The goal of researchers is not to reject the hypothesis. But it is evident that a perfect statistical model is always associated with the failure to reject the null hypothesis.

How do you Find the Null Hypothesis?

The null hypothesis says there is no correlation between the measured event (the dependent variable) and the independent variable. We don’t have to believe that the null hypothesis is true to test it. On the contrast, you will possibly assume that there is a connection between a set of variables ( dependent and independent).

When is Null Hypothesis Rejected?

The null hypothesis is rejected using the P-value approach. If the P-value is less than or equal to the α, there should be a rejection of the null hypothesis in favour of the alternate hypothesis. In case, if P-value is greater than α, the null hypothesis is not rejected.

Null Hypothesis and Alternative Hypothesis

Now, let us discuss the difference between the null hypothesis and the alternative hypothesis.

Null Hypothesis Examples

Here, some of the examples of the null hypothesis are given below. Go through the below ones to understand the concept of the null hypothesis in a better way.

If a medicine reduces the risk of cardiac stroke, then the null hypothesis should be “the medicine does not reduce the chance of cardiac stroke”. This testing can be performed by the administration of a drug to a certain group of people in a controlled way. If the survey shows that there is a significant change in the people, then the hypothesis is rejected.

Few more examples are:

1). Are there is 100% chance of getting affected by dengue?

Ans: There could be chances of getting affected by dengue but not 100%.

2). Do teenagers are using mobile phones more than grown-ups to access the internet?

Ans: Age has no limit on using mobile phones to access the internet.

3). Does having apple daily will not cause fever?

Ans: Having apple daily does not assure of not having fever, but increases the immunity to fight against such diseases.

4). Do the children more good in doing mathematical calculations than grown-ups?

Ans: Age has no effect on Mathematical skills.

In many common applications, the choice of the null hypothesis is not automated, but the testing and calculations may be automated. Also, the choice of the null hypothesis is completely based on previous experiences and inconsistent advice. The choice can be more complicated and based on the variety of applications and the diversity of the objectives. 

The main limitation for the choice of the null hypothesis is that the hypothesis suggested by the data is based on the reasoning which proves nothing. It means that if some hypothesis provides a summary of the data set, then there would be no value in the testing of the hypothesis on the particular set of data. 

Frequently Asked Questions on Null Hypothesis

What is meant by the null hypothesis.

In Statistics, a null hypothesis is a type of hypothesis which explains the population parameter whose purpose is to test the validity of the given experimental data.

What are the benefits of hypothesis testing?

Hypothesis testing is defined as a form of inferential statistics, which allows making conclusions from the entire population based on the sample representative.

When a null hypothesis is accepted and rejected?

The null hypothesis is either accepted or rejected in terms of the given data. If P-value is less than α, then the null hypothesis is rejected in favor of the alternative hypothesis, and if the P-value is greater than α, then the null hypothesis is accepted in favor of the alternative hypothesis.

Why is the null hypothesis important?

The importance of the null hypothesis is that it provides an approximate description of the phenomena of the given data. It allows the investigators to directly test the relational statement in a research study.

How to accept or reject the null hypothesis in the chi-square test?

If the result of the chi-square test is bigger than the critical value in the table, then the data does not fit the model, which represents the rejection of the null hypothesis.

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How to choose the null and alternative hypothesis?

I'm practicing with the hypothesis test and I find myself in trouble with the decision about how to set a null and an alternative hypothesis. My main issue is to determine, in every situation, a "general rule" on how I can decide correctly which is the null and which is the alternative hypothesis.. can someone help me?

Here is an example: As an established scholar, you are requested to evaluate if Customer Relationship Management affects the financial performance of firms. The main issue will be solved by means of a test of hypothesis. Two hypothesis will be tested one against the other: CRM is related to performance, CRM is not related.

• hypothesis-testing

• 1 $\begingroup$ This tutorial video is a very good explanation if you want to learn about hypothesis testing in an intuitive approach - youtube.com/watch?v=UApFKiK4Hi8 $\endgroup$ –  London guy Commented Nov 9, 2014 at 18:58

3 Answers 3

The rule for the proper formulation of a hypothesis test is that the alternative or research hypothesis is the statement that, if true, is strongly supported by the evidence furnished by the data.

The null hypothesis is generally the complement of the alternative hypothesis. Frequently, it is (or contains) the assumption that you are making about how the data are distributed in order to calculate the test statistic.

Here are a few examples to help you understand how these are properly chosen.

Suppose I am an epidemiologist in public health, and I'm investigating whether the incidence of smoking among a certain ethnic group is greater than the population as a whole, and therefore there is a need to target anti-smoking campaigns for this sub-population through greater community outreach and education. From previous studies that have been published in the literature, I find that the incidence among the general population is $p_0$. I can then go about collecting sample data (that's actually the hard part!) to test $$H_0 : p = p_0 \quad \mathrm{vs.} \quad H_a : p > p_0.$$ This is a one-sided binomial proportion test. $H_a$ is the statement that, if it were true, would need to be strongly supported by the data we collected. It is the statement that carries the burden of proof . This is because any conclusion we draw from the test is conditional upon assuming that the null is true: either $H_a$ is accepted, or the test is inconclusive and there is insufficient evidence from the data to suggest $H_a$ is true. The choice of $H_0$ reflects the underlying assumption that there is no difference in the smoking rates of the sub-population compared to the whole.

Now suppose I am a researcher investigating a new drug that I believe to be equally effective to an existing standard of treatment, but with fewer side effects and therefore a more desirable safety profile. I would like to demonstrate the equal efficacy by conducting a bioequivalence test. If $\mu_0$ is the mean existing standard treatment effect, then my hypothesis might look like this: $$H_0 : |\mu - \mu_0| \ge \Delta \quad \mathrm{vs.} \quad H_a : |\mu - \mu_0| < \Delta,$$ for some choice of margin $\Delta$ that I consider to be clinically significant. For example, a clinician might say that two treatments are sufficiently bioequivalent if there is less than a $\Delta = 10\%$ difference in treatment effect. Note again that $H_a$ is the statement that carries the burden of proof: the data we collect must strongly support it, in order for us to accept it; otherwise, it could still be true but we don't have the evidence to support the claim .

Now suppose I am doing an analysis for a small business owner who sells three products $A$, $B$, $C$. They suspect that there is a statistically significant preference for these three products. Then my hypothesis is $$H_0 : \mu_A = \mu_B = \mu_C \quad \mathrm{vs.} \quad H_a : \exists i \ne j \text{ such that } \mu_i \ne \mu_j.$$ Really, all that $H_a$ is saying is that there are two means that are not equal to each other, which would then suggest that some difference in preference exists.

• $\begingroup$ Thank you so much.. Also your response has been of great help :D $\endgroup$ –  RedViper16 Commented Nov 10, 2014 at 21:25
• $\begingroup$ The third example seems worse than the first two in that, in reality, with a finite size sample, no two distinct products will almost never sell at exactly same amount and $H_0$ as formulated above will almost always be rejected? $\endgroup$ –  qazwsx Commented Nov 21, 2015 at 20:23
• 1 $\begingroup$ @qazwsx What one believes about the likelihood of rejecting the null hypothesis has no bearing on the appropriate construction of the hypothesis test that correctly tests the inference of interest. That is to say, if one is interested in demonstrating with a high degree of confidence that there is a difference between two (or more) means, then the way to test this hypothesis is to use the structure specified in (3), no matter what the data says , because the hypothesis is necessarily pre-specified . $\endgroup$ –  heropup Commented Nov 22, 2015 at 5:22
• 1 $\begingroup$ @MSIS You've omitted a critical detail that is present in my original characterization: "the alternative hypothesis is the statement that, if true , is strongly supported by the evidence furnished by the data." You want to choose the alternative in such a way that if you conduct the test and reject the null in favor of the alternative, a high standard of evidence has been met to support this decision. But this does not mean the null is "usually" true or that the alternative "should be" true. It is like the legal principle of "innocent until proven guilty beyond a reasonable doubt." $\endgroup$ –  heropup Commented Dec 4, 2019 at 8:44
• 2 $\begingroup$ @MSIS (cont.) To illustrate the analogy, in a criminal court of law, a defendant is assumed innocent of a crime, and the prosecution must establish guilt through presenting evidence. The strength of this evidence must be such that no reasonable logic could exculpate the defendant. Otherwise, the burden of proof is not met. That is not to say the defendant is truly innocent: it simply means the evidence is lacking to establish guilt beyond a reasonable doubt. Similarly, failure to reject the null does not imply the null is true; rather, the data is simply lacking. $\endgroup$ –  heropup Commented Dec 4, 2019 at 8:48

The null hypothesis is nearly always "something didn't happen" or "there is no effect" or "there is no relationship" or something similar. But it need not be this.

In your case, the null would be "there is no relationship between CRM and performance"

The usual method is to test the null at some significance level (most often, 0.05). Whether this is a good method is another matter, but it is what is commonly done.

In science proofs, you can never prove anything, you can only demonstrate that your model describes the data better than another model. You want your alternate hypothesis to come from the new model under test, and the null hypothesis to be from a different model.

The null hypothesis should come from a model which others would choose to use when challenging your scientific claims! The most common pattern for a scientific claim is "I think that X is a factor in process Y. If everyone already believes X is a factor in the process, then there is nothing to prove, and everyone can just go out and talk about it over drinks. Scientific arguments with null hypothesis are interesting because, if someone takes the opposing view, "X is not a factor in process Y, then there is a disagreement. This is where science does its thing.

If you believe "X is a factor in process Y" enough to run an experiment, you should generally know what you're looking to see in the results. So now your phrase becomes "X is a factor in process Y, producing visible outcome Z."

This is where you pick your null hypothesis. If someone believes X is not a factor, and your experiment does indeed show Z, then they need an explanation for Z. With your choice of null hypothesis, you are effectively challenging their explanation . The dead simplest explanation is always "Z was caused by random chance because science is based on statistics." Accordingly, most null hypothesis are in the form of "The outcome should be predicted using the previously accepted model plus some random chance to account for statistics.

Both hypothesis should be phrased in terms of the visible outcome, NOT the model you intend to prove. [note] You never start with an alternate hypothesis of "I believe X is a factor." You phrase it "I expect to see this result when I observe Z." The null hypothesis will be phrased similarly, "The status quo predicts that we will see this different result when I observe Z." There is always a statistical phrasing in there such as "I expect to observe a normal distribution on Z when I do this experiment over and over." Once you observe results that defend your alternate hypothesis and reject the null hypothsis, you are THEN in a position to make claims about the validity of your model.

[note] This bolded statement is my opinion, but I feel confident enough in its wording choice to post it. The hypotheses draw a strong line between the intuitive portion of the science, and the data and analysis of the science. If your phrasing is too close to the model, it becomes hard to separate the model from the data, and makes it harder for the next scientist to use your data

In the case of our simple model with process Y and visible outcome Z, the existing belief is that Z will fit a distribution that everyone is already comfortable with, such as "the randomness expected by your particular laboratory equipment setup" or "the purity of the reagents used in the experiment." When you "reject the null hypothesis" what you are saying is most literally, "I have run this experiment, and it is so tremendously unlikely that random chance generated the observed behavior, that everybody should start considering that maybe there's more to this than meets the eye."

The alternative hypothesis is what you offer to the world to replace the null hypothesis . It is one thing to go do experiments to poke at holes in other's models, but that doesn't promote science nearly as well as poking holes in other's models and then replacing them with new models that do a better job.

With the null and alternate hypothesis, you are trying to challenge the current conventional thinking of the day. Choose the hypotheses so that they effectively declare "Here is a result everybody would expect (null hypothesis). However, I actually went out and did the experiment and gathered data, and it is VERY unlikely that the null hypothesis is true. Here is the result I expected (the alternate hypothesis). Nobody expected this hypothesis to be true but me, but when I gathered the data and did the statistics, it is very likely that my model does a better job of describing reality than the existing model . Accordingly, I reject the null hypothesis, accept my hypothesis, and challenge my fellow scientists to work from this new data."

And the fellow scientists are free to:

• Rejoice and accept your data and model with open arms.
• Ignore your data or model (sorry, it happens... welcome to real life)
• Reject your data, and spend their effort running experiments to show different data (It is very common for the science community to say "We do not trust your sample size of 10. We are going to redo your experiment with a sample size of 1000.)
• Accept your data, but not your model. They then must spend their effort generating a new model which explains the data in a different manner.

The last outcome causes strife and bickering, but is ABSOLUTELY part of the scientific process. By using the scientific method to publish your results, you accept that others are free to use the scientific method to contradict your results. They will do so, and publish their results.

At this point, the scientific community will make a political decision: who has to go out and spend the money to test their model, and whose model do we accept. TYPICALLY, because you published the model and the data first, and they are refuting your data, the onus is on them to run the experiments which proves why their model is better than yours. But this is now WELL beyond the hypothesis that caused the strife in the first place, so I leave you to experience them in your lifetime!

• $\begingroup$ Thank you so much for your response. You have been very precise and of help! I've now a stronger idea of what this hypothesis means :D $\endgroup$ –  RedViper16 Commented Nov 10, 2014 at 21:24

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• Published: 27 June 2024

Tree phytochemical diversity and herbivory are higher in the tropics

• Lu Sun   ORCID: orcid.org/0000-0002-7688-6472 1 ,
• Yunyun He 1 , 2 ,
• Min Cao 1 ,
• Xuezhao Wang 1 , 2 ,
• Xiang Zhou   ORCID: orcid.org/0000-0002-8393-1091 3 ,
• Jie Yang   ORCID: orcid.org/0000-0002-4444-8240 1 &
• Nathan G. Swenson   ORCID: orcid.org/0000-0003-3819-9767 4  

Nature Ecology & Evolution ( 2024 ) Cite this article

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• Community ecology
• Forest ecology

A long-standing but poorly tested hypothesis in plant ecology and evolution is that biotic interactions play a more important role in producing and maintaining species diversity in the tropics than in the temperate zone. A core prediction of this hypothesis is that tropical plants deploy a higher diversity of phytochemicals within and across communities because they experience more herbivore pressure than temperate plants. However, simultaneous comparisons of phytochemical diversity and herbivore pressure in plant communities from the tropical to the temperate zone are lacking. Here we provide clear support for this prediction by examining phytochemical diversity and herbivory in 60 tree communities ranging from species-rich tropical rainforests to species-poor subalpine forests. Using a community metabolomics approach, we show that phytochemical diversity is higher within and among tropical tree communities than within and among subtropical and subalpine communities, and that herbivore pressure and specialization are highest in the tropics. Furthermore, we show that the phytochemical similarity of trees has little phylogenetic signal, indicating rapid divergence between closely related species. In sum, we provide several lines of evidence from entire tree communities showing that biotic interactions probably play an increasingly important role in generating and maintaining tree diversity in the lower latitudes.

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Data availability.

The MS data (.mzML) have been deposited in the MassIVE public repository and are available under accession number MSV000092950 . The datasets analysed in the current study, including the molecular network, sample–sample chemical structural and compositional similarity, plot-species-abundance community data, phytochemical richness and the phylogenetic tree of 206 tree species, are available via Figshare at https://doi.org/10.6084/m9.figshare.22758269 (ref. 69 ).

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Acknowledgements

This research was supported by the NSFC China–US Dimensions of Biodiversity Grant (DEB: no. 32061123003 to M.C.); the National Natural Science Foundation of China (grant nos. 32201318 to L.S. and 31870410 to J.Y.); the Chinese Academy of Sciences Youth Innovation Promotion Association (grant no. Y202080 to J.Y.); the Distinguished Youth Scholar of Yunnan (grant no. 202101AV070005 to J.Y.); the Ten Thousand Talent Plans for Young Top-Notch Talents of Yunnan Province (grant no. YNWR-QNBJ-2018-309 to J.Y.); a Postdoctoral Fellowship of Xishuangbanna Tropical Botanical Garden, CAS, to L.S.; the Postdoctoral Science Foundation of Yunnan Province to L.S.; the 14th Five-Year Plan of the Xishuangbanna Tropical Botanical Garden, Chinese Academy of Sciences (grant nos. XTBG-1450101 and E3ZKFF2B01 to J.Y.); and an NSF US–China Dimensions of Biodiversity Grant (DEB: no. 2124466) to N.G.S. We acknowledge support from Xishuangbanna Station for Tropical Rain Forest Ecosystem Studies, Ailaoshan Station for Subtropical Forest Ecosystem Studies and Lijiang Forest Ecosystem Research Station. We thank the Molecular Biology Experiment Center in Germplasm Bank of Wild Species, Chinese Academy of Sciences, for facilitating the extraction of plant metabolites, and the State Key Laboratory of Phytochemistry and Plant Resources in West China, Chinese Academy of Sciences, for performing the UHPLC–MS/MS analysis. We thank J. Wang, C. Xu, P. Song, T. Liang and many local residents for their assistance in collecting leaf samples. We also thank J. Yang, H. Liu and Y. Tan for their kind assistance during extracting plant metabolites and metabolite analysis.

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CAS Key Laboratory of Tropical Forest Ecology, Xishuangbanna Tropical Botanical Garden, Chinese Academy of Sciences, Mengla, China

Lu Sun, Yunyun He, Min Cao, Xuezhao Wang & Jie Yang

University of Chinese Academy Sciences, Beijing, China

Yunyun He & Xuezhao Wang

School of Ethnic Medicine, Key Lab of Chemistry in Ethnic Medicinal Resources, State Ethnic Affairs Commission & Ministry of Education of China, Yunnan Minzu University, Kunming, China

Department of Biological Sciences, University of Notre Dame, Notre Dame, IN, USA

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Contributions

J.Y., L.S. and N.G.S. designed the study. M.C. set up the forest inventory plots. L.S., X.Z., Y.H. and X.W. collected and processed the metabolomics data. L.S., Y.H. and X.W. collected and processed the leaf samples. L.S. and J.Y. analysed the data with input from all authors. J.Y., N.G.S. and L.S. wrote the paper. All authors provided feedback on the final version of the paper.

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Correspondence to Jie Yang .

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Extended data

Extended data fig. 1 observed phytochemical alpha diversity for seven biosynthetic pathway categories within each climatic zone (tropical, sub-tropical and sub-alpine) with diverse q exponents (qorder = 0, 1, 2)..

( a ) terpenoids (n = 20 in tropical zone, n = 20 in sub-tropical zone, n = 20 in sub-alpine zone, for one of q exponents), ( b ) shikimates and phenylpropanoids (n = 20 in tropical zone, n = 20 in sub-tropical zone, n = 20 in sub-alpine zone, for one of q exponents), ( c ) polyketides (n = 20 in tropical zone, n = 20 in sub-tropical zone, n = 20 in sub-alpine zone, for one of q exponents), ( d ) alkaloids (n = 20 in tropical zone, n = 20 in sub-tropical zone, n = 20 in sub-alpine zone, for one of q exponents), ( e ) fatty acids (n = 20 in tropical zone, n = 20 in sub-tropical zone, n = 17 in sub-alpine zone, for one of q exponents), ( f ) amino acids/peptides (n = 18 in tropical zone, n = 19 in sub-tropical zone, n = 12 in sub-alpine zone, for one of q exponents), ( g ) carbohydrates (n = 17 in tropical zone, n = 20 in sub-tropical zone, n = 17 in sub-alpine zone, for one of q exponents). In all panels, the significance of difference of phytochemical alpha diversity across forest type pairs were tested using a one-way ANOVA with a post-hoc Tukey test. In boxplots: the centre line represents the median; the lower and upper hinges correspond to the 25th and 75th percentiles; the lower and upper whiskers extend to the lowest and highest points to a limit of 1.5× the interquartile range from the closest hinge.

Extended Data Fig. 2 Observed phytochemical beta diversity for seven biosynthetic pathway categories within each climatic zone (tropical, sub-tropical and sub-alpine) with diverse q exponents (Qorder = 0, 1, 2).

( a ) terpenoids (n = 190 in tropical zone, n = 190 in sub-tropical zone, n = 190 in sub-alpine zone, for one of q exponents), ( b ) shikimates and phenylpropanoids (n = 190 in tropical zone, n = 190 in sub-tropical zone, n = 190 in sub-alpine zone, for one of q exponents), ( c ) polyketides (n = 190 in tropical zone, n = 190 in sub-tropical zone, n = 190 in sub-alpine zone, for one of q exponents), ( d ) alkaloids (n = 190 in tropical zone, n = 190 in sub-tropical zone, n = 190 in sub-alpine zone, for one of q exponents), ( e ) fatty acids (n = 190 in tropical zone, n = 190 in sub-tropical zone, n = 136 in sub-alpine zone, for one of q exponents), ( f ) amino acids/peptides (n = 153 in tropical zone, n = 171 in sub-tropical zone, n = 66 in sub-alpine zone, for one of q exponents), ( g ) carbohydrates (n = 136 in tropical zone, n = 190 in sub-tropical zone, n = 136 in sub-alpine zone, for one of q exponents). In all panels, the significance of difference of phytochemical beta diversity across forest type pairs were tested using a one-way ANOVA with a post-hoc Tukey test. In boxplots: the centre line represents the median; the lower and upper hinges correspond to the 25th and 75th percentiles; the lower and upper whiskers extend to the lowest and highest points to a limit of 1.5× the interquartile range from the closest hinge.

Extended Data Fig. 3 Distance-decay curves for the whole plant specialized metabolites with diverse q exponents of 0, 1, 2.

The rate of decay (slope) and corresponding significance level were estimated by regressing the chemical similarity against elevational distance via generalized linear model with link log and a quasi-binomial family. The trend lines represent linear fits from regressions, and coloured shaded areas indicate 95% confidence interval (CI) of the prediction. Colours denote whole study region (grey), tropical zone (red), sub-tropical zone (blue) and sub-alpine zone (yellow). Panels, a, d, g and j show the slope of the relationship when q exponents is 0. Panels, b, e, h and k . show the slope of the relationship when q exponents is 1. Panels, c, f, i and l show the slope of the relationship when q exponents is 2.

Extended Data Fig. 4 Distance-decay curves for the plant specialized metabolites on terpenoids with diverse q exponents of 0, 1, 2.

Extended data fig. 5 distance-decay curves for the plant specialized metabolites on shikimates and phenylpropanoids with diverse q exponents of 0, 1, 2., extended data fig. 6 distance-decay curves for the plant specialized metabolites on polyketides with diverse q exponents of 0, 1, 2..

The rate of decay (slope) and corresponding significance level were estimated by regressing the chemical similarity against elevational distance via generalized linear model with link log and a quasi-binomial family. The trend lines represent linear fits from regressions, and coloured shaded areas indicate 95% confidence interval (CI) of the prediction. Colours denote whole study region (grey), tropical zone (red), sub-tropical zone (blue), sub-alpine zone (yellow). Panels, a, d, g and j show the slope of the relationship when q exponents is 0. Panels, b, e, h and k . show the slope of the relationship when q exponents is 1. Panels, c, f, i and l show the slope of the relationship when q exponents is 2.

Extended Data Fig. 7 Distance-decay curves for the plant specialized metabolites on alkaloids with diverse q exponents of 0, 1, 2.

Extended data fig. 8 distance-decay curves for the plant specialized metabolites on fatty acids with diverse q exponents of 0, 1, 2., extended data fig. 9 distance-decay curves for the plant specialized metabolites on amino acids/ peptides with diverse q exponents of 0, 1, 2., extended data fig. 10 distance-decay curves for the plant specialized metabolites on carbohydrates with diverse q exponents of 0, 1, 2., supplementary information, supplementary information.

Supplementary Figs. 1–6, Tables 1–7, materials and methods.

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Sun, L., He, Y., Cao, M. et al. Tree phytochemical diversity and herbivory are higher in the tropics. Nat Ecol Evol (2024). https://doi.org/10.1038/s41559-024-02444-2

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DOI : https://doi.org/10.1038/s41559-024-02444-2

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