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• How to Write a Strong Hypothesis | Steps & Examples

How to Write a Strong Hypothesis | Steps & Examples

Published on May 6, 2022 by Shona McCombes . Revised on November 20, 2023.

A hypothesis is a statement that can be tested by scientific research. If you want to test a relationship between two or more variables, you need to write hypotheses before you start your experiment or data collection .

Example: Hypothesis

Daily apple consumption leads to fewer doctor’s visits.

Table of contents

What is a hypothesis, developing a hypothesis (with example), hypothesis examples, other interesting articles, frequently asked questions about writing hypotheses.

A hypothesis states your predictions about what your research will find. It is a tentative answer to your research question that has not yet been tested. For some research projects, you might have to write several hypotheses that address different aspects of your research question.

A hypothesis is not just a guess – it should be based on existing theories and knowledge. It also has to be testable, which means you can support or refute it through scientific research methods (such as experiments, observations and statistical analysis of data).

Variables in hypotheses

Hypotheses propose a relationship between two or more types of variables .

• An independent variable is something the researcher changes or controls.
• A dependent variable is something the researcher observes and measures.

If there are any control variables , extraneous variables , or confounding variables , be sure to jot those down as you go to minimize the chances that research bias  will affect your results.

In this example, the independent variable is exposure to the sun – the assumed cause . The dependent variable is the level of happiness – the assumed effect .

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Step 1. ask a question.

Writing a hypothesis begins with a research question that you want to answer. The question should be focused, specific, and researchable within the constraints of your project.

Step 2. Do some preliminary research

Your initial answer to the question should be based on what is already known about the topic. Look for theories and previous studies to help you form educated assumptions about what your research will find.

At this stage, you might construct a conceptual framework to ensure that you’re embarking on a relevant topic . This can also help you identify which variables you will study and what you think the relationships are between them. Sometimes, you’ll have to operationalize more complex constructs.

Step 3. Formulate your hypothesis

Now you should have some idea of what you expect to find. Write your initial answer to the question in a clear, concise sentence.

4. Refine your hypothesis

You need to make sure your hypothesis is specific and testable. There are various ways of phrasing a hypothesis, but all the terms you use should have clear definitions, and the hypothesis should contain:

• The relevant variables
• The specific group being studied
• The predicted outcome of the experiment or analysis

5. Phrase your hypothesis in three ways

To identify the variables, you can write a simple prediction in  if…then form. The first part of the sentence states the independent variable and the second part states the dependent variable.

In academic research, hypotheses are more commonly phrased in terms of correlations or effects, where you directly state the predicted relationship between variables.

If you are comparing two groups, the hypothesis can state what difference you expect to find between them.

6. Write a null hypothesis

If your research involves statistical hypothesis testing , you will also have to write a null hypothesis . The null hypothesis is the default position that there is no association between the variables. The null hypothesis is written as H 0 , while the alternative hypothesis is H 1 or H a .

• H 0 : The number of lectures attended by first-year students has no effect on their final exam scores.
• H 1 : The number of lectures attended by first-year students has a positive effect on their final exam scores.

If you want to know more about the research process , methodology , research bias , or statistics , make sure to check out some of our other articles with explanations and examples.

• Sampling methods
• Simple random sampling
• Stratified sampling
• Cluster sampling
• Likert scales
• Reproducibility

 Statistics

• Null hypothesis
• Statistical power
• Probability distribution
• Effect size
• Poisson distribution

Research bias

• Optimism bias
• Cognitive bias
• Implicit bias
• Hawthorne effect
• Anchoring bias
• Explicit bias

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A hypothesis is not just a guess — it should be based on existing theories and knowledge. It also has to be testable, which means you can support or refute it through scientific research methods (such as experiments, observations and statistical analysis of data).

Null and alternative hypotheses are used in statistical hypothesis testing . The null hypothesis of a test always predicts no effect or no relationship between variables, while the alternative hypothesis states your research prediction of an effect or relationship.

Hypothesis testing is a formal procedure for investigating our ideas about the world using statistics. It is used by scientists to test specific predictions, called hypotheses , by calculating how likely it is that a pattern or relationship between variables could have arisen by chance.

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Home » What is a Hypothesis – Types, Examples and Writing Guide

What is a Hypothesis – Types, Examples and Writing Guide

Table of Contents

Definition:

Hypothesis is an educated guess or proposed explanation for a phenomenon, based on some initial observations or data. It is a tentative statement that can be tested and potentially proven or disproven through further investigation and experimentation.

Hypothesis is often used in scientific research to guide the design of experiments and the collection and analysis of data. It is an essential element of the scientific method, as it allows researchers to make predictions about the outcome of their experiments and to test those predictions to determine their accuracy.

Types of Hypothesis

Types of Hypothesis are as follows:

Research Hypothesis

A research hypothesis is a statement that predicts a relationship between variables. It is usually formulated as a specific statement that can be tested through research, and it is often used in scientific research to guide the design of experiments.

Null Hypothesis

The null hypothesis is a statement that assumes there is no significant difference or relationship between variables. It is often used as a starting point for testing the research hypothesis, and if the results of the study reject the null hypothesis, it suggests that there is a significant difference or relationship between variables.

Alternative Hypothesis

An alternative hypothesis is a statement that assumes there is a significant difference or relationship between variables. It is often used as an alternative to the null hypothesis and is tested against the null hypothesis to determine which statement is more accurate.

Directional Hypothesis

A directional hypothesis is a statement that predicts the direction of the relationship between variables. For example, a researcher might predict that increasing the amount of exercise will result in a decrease in body weight.

Non-directional Hypothesis

A non-directional hypothesis is a statement that predicts the relationship between variables but does not specify the direction. For example, a researcher might predict that there is a relationship between the amount of exercise and body weight, but they do not specify whether increasing or decreasing exercise will affect body weight.

Statistical Hypothesis

A statistical hypothesis is a statement that assumes a particular statistical model or distribution for the data. It is often used in statistical analysis to test the significance of a particular result.

Composite Hypothesis

A composite hypothesis is a statement that assumes more than one condition or outcome. It can be divided into several sub-hypotheses, each of which represents a different possible outcome.

Empirical Hypothesis

An empirical hypothesis is a statement that is based on observed phenomena or data. It is often used in scientific research to develop theories or models that explain the observed phenomena.

Simple Hypothesis

A simple hypothesis is a statement that assumes only one outcome or condition. It is often used in scientific research to test a single variable or factor.

Complex Hypothesis

A complex hypothesis is a statement that assumes multiple outcomes or conditions. It is often used in scientific research to test the effects of multiple variables or factors on a particular outcome.

Applications of Hypothesis

Hypotheses are used in various fields to guide research and make predictions about the outcomes of experiments or observations. Here are some examples of how hypotheses are applied in different fields:

• Science : In scientific research, hypotheses are used to test the validity of theories and models that explain natural phenomena. For example, a hypothesis might be formulated to test the effects of a particular variable on a natural system, such as the effects of climate change on an ecosystem.
• Medicine : In medical research, hypotheses are used to test the effectiveness of treatments and therapies for specific conditions. For example, a hypothesis might be formulated to test the effects of a new drug on a particular disease.
• Psychology : In psychology, hypotheses are used to test theories and models of human behavior and cognition. For example, a hypothesis might be formulated to test the effects of a particular stimulus on the brain or behavior.
• Sociology : In sociology, hypotheses are used to test theories and models of social phenomena, such as the effects of social structures or institutions on human behavior. For example, a hypothesis might be formulated to test the effects of income inequality on crime rates.
• Business : In business research, hypotheses are used to test the validity of theories and models that explain business phenomena, such as consumer behavior or market trends. For example, a hypothesis might be formulated to test the effects of a new marketing campaign on consumer buying behavior.
• Engineering : In engineering, hypotheses are used to test the effectiveness of new technologies or designs. For example, a hypothesis might be formulated to test the efficiency of a new solar panel design.

How to write a Hypothesis

Here are the steps to follow when writing a hypothesis:

Identify the Research Question

The first step is to identify the research question that you want to answer through your study. This question should be clear, specific, and focused. It should be something that can be investigated empirically and that has some relevance or significance in the field.

Conduct a Literature Review

Before writing your hypothesis, it’s essential to conduct a thorough literature review to understand what is already known about the topic. This will help you to identify the research gap and formulate a hypothesis that builds on existing knowledge.

Determine the Variables

The next step is to identify the variables involved in the research question. A variable is any characteristic or factor that can vary or change. There are two types of variables: independent and dependent. The independent variable is the one that is manipulated or changed by the researcher, while the dependent variable is the one that is measured or observed as a result of the independent variable.

Formulate the Hypothesis

Based on the research question and the variables involved, you can now formulate your hypothesis. A hypothesis should be a clear and concise statement that predicts the relationship between the variables. It should be testable through empirical research and based on existing theory or evidence.

Write the Null Hypothesis

The null hypothesis is the opposite of the alternative hypothesis, which is the hypothesis that you are testing. The null hypothesis states that there is no significant difference or relationship between the variables. It is important to write the null hypothesis because it allows you to compare your results with what would be expected by chance.

Refine the Hypothesis

After formulating the hypothesis, it’s important to refine it and make it more precise. This may involve clarifying the variables, specifying the direction of the relationship, or making the hypothesis more testable.

Examples of Hypothesis

Here are a few examples of hypotheses in different fields:

• Psychology : “Increased exposure to violent video games leads to increased aggressive behavior in adolescents.”
• Biology : “Higher levels of carbon dioxide in the atmosphere will lead to increased plant growth.”
• Sociology : “Individuals who grow up in households with higher socioeconomic status will have higher levels of education and income as adults.”
• Education : “Implementing a new teaching method will result in higher student achievement scores.”
• Marketing : “Customers who receive a personalized email will be more likely to make a purchase than those who receive a generic email.”
• Physics : “An increase in temperature will cause an increase in the volume of a gas, assuming all other variables remain constant.”
• Medicine : “Consuming a diet high in saturated fats will increase the risk of developing heart disease.”

Purpose of Hypothesis

The purpose of a hypothesis is to provide a testable explanation for an observed phenomenon or a prediction of a future outcome based on existing knowledge or theories. A hypothesis is an essential part of the scientific method and helps to guide the research process by providing a clear focus for investigation. It enables scientists to design experiments or studies to gather evidence and data that can support or refute the proposed explanation or prediction.

The formulation of a hypothesis is based on existing knowledge, observations, and theories, and it should be specific, testable, and falsifiable. A specific hypothesis helps to define the research question, which is important in the research process as it guides the selection of an appropriate research design and methodology. Testability of the hypothesis means that it can be proven or disproven through empirical data collection and analysis. Falsifiability means that the hypothesis should be formulated in such a way that it can be proven wrong if it is incorrect.

In addition to guiding the research process, the testing of hypotheses can lead to new discoveries and advancements in scientific knowledge. When a hypothesis is supported by the data, it can be used to develop new theories or models to explain the observed phenomenon. When a hypothesis is not supported by the data, it can help to refine existing theories or prompt the development of new hypotheses to explain the phenomenon.

When to use Hypothesis

Here are some common situations in which hypotheses are used:

• In scientific research , hypotheses are used to guide the design of experiments and to help researchers make predictions about the outcomes of those experiments.
• In social science research , hypotheses are used to test theories about human behavior, social relationships, and other phenomena.
• I n business , hypotheses can be used to guide decisions about marketing, product development, and other areas. For example, a hypothesis might be that a new product will sell well in a particular market, and this hypothesis can be tested through market research.

Characteristics of Hypothesis

Here are some common characteristics of a hypothesis:

• Testable : A hypothesis must be able to be tested through observation or experimentation. This means that it must be possible to collect data that will either support or refute the hypothesis.
• Falsifiable : A hypothesis must be able to be proven false if it is not supported by the data. If a hypothesis cannot be falsified, then it is not a scientific hypothesis.
• Clear and concise : A hypothesis should be stated in a clear and concise manner so that it can be easily understood and tested.
• Based on existing knowledge : A hypothesis should be based on existing knowledge and research in the field. It should not be based on personal beliefs or opinions.
• Specific : A hypothesis should be specific in terms of the variables being tested and the predicted outcome. This will help to ensure that the research is focused and well-designed.
• Tentative: A hypothesis is a tentative statement or assumption that requires further testing and evidence to be confirmed or refuted. It is not a final conclusion or assertion.
• Relevant : A hypothesis should be relevant to the research question or problem being studied. It should address a gap in knowledge or provide a new perspective on the issue.

Advantages of Hypothesis

Hypotheses have several advantages in scientific research and experimentation:

• Guides research: A hypothesis provides a clear and specific direction for research. It helps to focus the research question, select appropriate methods and variables, and interpret the results.
• Predictive powe r: A hypothesis makes predictions about the outcome of research, which can be tested through experimentation. This allows researchers to evaluate the validity of the hypothesis and make new discoveries.
• Facilitates communication: A hypothesis provides a common language and framework for scientists to communicate with one another about their research. This helps to facilitate the exchange of ideas and promotes collaboration.
• Efficient use of resources: A hypothesis helps researchers to use their time, resources, and funding efficiently by directing them towards specific research questions and methods that are most likely to yield results.
• Provides a basis for further research: A hypothesis that is supported by data provides a basis for further research and exploration. It can lead to new hypotheses, theories, and discoveries.
• Increases objectivity: A hypothesis can help to increase objectivity in research by providing a clear and specific framework for testing and interpreting results. This can reduce bias and increase the reliability of research findings.

Limitations of Hypothesis

Some Limitations of the Hypothesis are as follows:

• Limited to observable phenomena: Hypotheses are limited to observable phenomena and cannot account for unobservable or intangible factors. This means that some research questions may not be amenable to hypothesis testing.
• May be inaccurate or incomplete: Hypotheses are based on existing knowledge and research, which may be incomplete or inaccurate. This can lead to flawed hypotheses and erroneous conclusions.
• May be biased: Hypotheses may be biased by the researcher’s own beliefs, values, or assumptions. This can lead to selective interpretation of data and a lack of objectivity in research.
• Cannot prove causation: A hypothesis can only show a correlation between variables, but it cannot prove causation. This requires further experimentation and analysis.
• Limited to specific contexts: Hypotheses are limited to specific contexts and may not be generalizable to other situations or populations. This means that results may not be applicable in other contexts or may require further testing.
• May be affected by chance : Hypotheses may be affected by chance or random variation, which can obscure or distort the true relationship between variables.

About the author

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Science and the scientific method: Definitions and examples

Here's a look at the foundation of doing science — the scientific method.

The scientific method

Hypothesis, theory and law, a brief history of science, additional resources, bibliography.

Science is a systematic and logical approach to discovering how things in the universe work. It is also the body of knowledge accumulated through the discoveries about all the things in the universe. 

The word "science" is derived from the Latin word "scientia," which means knowledge based on demonstrable and reproducible data, according to the Merriam-Webster dictionary . True to this definition, science aims for measurable results through testing and analysis, a process known as the scientific method. Science is based on fact, not opinion or preferences. The process of science is designed to challenge ideas through research. One important aspect of the scientific process is that it focuses only on the natural world, according to the University of California, Berkeley . Anything that is considered supernatural, or beyond physical reality, does not fit into the definition of science.

When conducting research, scientists use the scientific method to collect measurable, empirical evidence in an experiment related to a hypothesis (often in the form of an if/then statement) that is designed to support or contradict a scientific theory .

"As a field biologist, my favorite part of the scientific method is being in the field collecting the data," Jaime Tanner, a professor of biology at Marlboro College, told Live Science. "But what really makes that fun is knowing that you are trying to answer an interesting question. So the first step in identifying questions and generating possible answers (hypotheses) is also very important and is a creative process. Then once you collect the data you analyze it to see if your hypothesis is supported or not."

The steps of the scientific method go something like this, according to Highline College :

• Make an observation or observations.
• Form a hypothesis — a tentative description of what's been observed, and make predictions based on that hypothesis.
• Test the hypothesis and predictions in an experiment that can be reproduced.
• Analyze the data and draw conclusions; accept or reject the hypothesis or modify the hypothesis if necessary.
• Reproduce the experiment until there are no discrepancies between observations and theory. "Replication of methods and results is my favorite step in the scientific method," Moshe Pritsker, a former post-doctoral researcher at Harvard Medical School and CEO of JoVE, told Live Science. "The reproducibility of published experiments is the foundation of science. No reproducibility — no science."

Some key underpinnings to the scientific method:

• The hypothesis must be testable and falsifiable, according to North Carolina State University . Falsifiable means that there must be a possible negative answer to the hypothesis.
• Research must involve deductive reasoning and inductive reasoning . Deductive reasoning is the process of using true premises to reach a logical true conclusion while inductive reasoning uses observations to infer an explanation for those observations.
• An experiment should include a dependent variable (which does not change) and an independent variable (which does change), according to the University of California, Santa Barbara .
• An experiment should include an experimental group and a control group. The control group is what the experimental group is compared against, according to Britannica .

The process of generating and testing a hypothesis forms the backbone of the scientific method. When an idea has been confirmed over many experiments, it can be called a scientific theory. While a theory provides an explanation for a phenomenon, a scientific law provides a description of a phenomenon, according to The University of Waikato . One example would be the law of conservation of energy, which is the first law of thermodynamics that says that energy can neither be created nor destroyed. 

A law describes an observed phenomenon, but it doesn't explain why the phenomenon exists or what causes it. "In science, laws are a starting place," said Peter Coppinger, an associate professor of biology and biomedical engineering at the Rose-Hulman Institute of Technology. "From there, scientists can then ask the questions, 'Why and how?'"

Laws are generally considered to be without exception, though some laws have been modified over time after further testing found discrepancies. For instance, Newton's laws of motion describe everything we've observed in the macroscopic world, but they break down at the subatomic level.

This does not mean theories are not meaningful. For a hypothesis to become a theory, scientists must conduct rigorous testing, typically across multiple disciplines by separate groups of scientists. Saying something is "just a theory" confuses the scientific definition of "theory" with the layperson's definition. To most people a theory is a hunch. In science, a theory is the framework for observations and facts, Tanner told Live Science.

The earliest evidence of science can be found as far back as records exist. Early tablets contain numerals and information about the solar system , which were derived by using careful observation, prediction and testing of those predictions. Science became decidedly more "scientific" over time, however.

1200s: Robert Grosseteste developed the framework for the proper methods of modern scientific experimentation, according to the Stanford Encyclopedia of Philosophy. His works included the principle that an inquiry must be based on measurable evidence that is confirmed through testing.

1400s: Leonardo da Vinci began his notebooks in pursuit of evidence that the human body is microcosmic. The artist, scientist and mathematician also gathered information about optics and hydrodynamics.

1500s: Nicolaus Copernicus advanced the understanding of the solar system with his discovery of heliocentrism. This is a model in which Earth and the other planets revolve around the sun, which is the center of the solar system.

1600s: Johannes Kepler built upon those observations with his laws of planetary motion. Galileo Galilei improved on a new invention, the telescope, and used it to study the sun and planets. The 1600s also saw advancements in the study of physics as Isaac Newton developed his laws of motion.

1700s: Benjamin Franklin discovered that lightning is electrical. He also contributed to the study of oceanography and meteorology. The understanding of chemistry also evolved during this century as Antoine Lavoisier, dubbed the father of modern chemistry , developed the law of conservation of mass.

1800s: Milestones included Alessandro Volta's discoveries regarding electrochemical series, which led to the invention of the battery. John Dalton also introduced atomic theory, which stated that all matter is composed of atoms that combine to form molecules. The basis of modern study of genetics advanced as Gregor Mendel unveiled his laws of inheritance. Later in the century, Wilhelm Conrad Röntgen discovered X-rays , while George Ohm's law provided the basis for understanding how to harness electrical charges.

1900s: The discoveries of Albert Einstein , who is best known for his theory of relativity, dominated the beginning of the 20th century. Einstein's theory of relativity is actually two separate theories. His special theory of relativity, which he outlined in a 1905 paper, " The Electrodynamics of Moving Bodies ," concluded that time must change according to the speed of a moving object relative to the frame of reference of an observer. His second theory of general relativity, which he published as " The Foundation of the General Theory of Relativity ," advanced the idea that matter causes space to curve.

In 1952, Jonas Salk developed the polio vaccine , which reduced the incidence of polio in the United States by nearly 90%, according to Britannica . The following year, James D. Watson and Francis Crick discovered the structure of DNA , which is a double helix formed by base pairs attached to a sugar-phosphate backbone, according to the National Human Genome Research Institute .

2000s: The 21st century saw the first draft of the human genome completed, leading to a greater understanding of DNA. This advanced the study of genetics, its role in human biology and its use as a predictor of diseases and other disorders, according to the National Human Genome Research Institute .

• This video from City University of New York delves into the basics of what defines science.
• Learn about what makes science science in this book excerpt from Washington State University .
• This resource from the University of Michigan — Flint explains how to design your own scientific study.

Merriam-Webster Dictionary, Scientia. 2022. https://www.merriam-webster.com/dictionary/scientia

University of California, Berkeley, "Understanding Science: An Overview." 2022. ​​ https://undsci.berkeley.edu/article/0_0_0/intro_01  

Highline College, "Scientific method." July 12, 2015. https://people.highline.edu/iglozman/classes/astronotes/scimeth.htm  

North Carolina State University, "Science Scripts." https://projects.ncsu.edu/project/bio183de/Black/science/science_scripts.html  

University of California, Santa Barbara. "What is an Independent variable?" October 31,2017. http://scienceline.ucsb.edu/getkey.php?key=6045  

Encyclopedia Britannica, "Control group." May 14, 2020. https://www.britannica.com/science/control-group  

The University of Waikato, "Scientific Hypothesis, Theories and Laws." https://sci.waikato.ac.nz/evolution/Theories.shtml  

Stanford Encyclopedia of Philosophy, Robert Grosseteste. May 3, 2019. https://plato.stanford.edu/entries/grosseteste/  

Encyclopedia Britannica, "Jonas Salk." October 21, 2021. https://www.britannica.com/ biography /Jonas-Salk

National Human Genome Research Institute, "​Phosphate Backbone." https://www.genome.gov/genetics-glossary/Phosphate-Backbone  

National Human Genome Research Institute, "What is the Human Genome Project?" https://www.genome.gov/human-genome-project/What  

‌ Live Science contributor Ashley Hamer updated this article on Jan. 16, 2022.

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Scientific Method: Step 3: HYPOTHESIS

• Step 1: QUESTION
• Step 2: RESEARCH
• Step 3: HYPOTHESIS
• Step 4: EXPERIMENT
• Step 5: DATA
• Step 6: CONCLUSION

Step 3: State your hypothesis

Now it's time to state your hypothesis . The hypothesis is an educated guess as to what will happen during your experiment. 

The hypothesis is often written using the words "IF" and "THEN." For example, " If I do not study, then I will fail the test." The "if' and "then" statements reflect your independent and dependent variables . 

The hypothesis should relate back to your original question and must be testable .

A word about variables...

Your experiment will include variables to measure and to explain any cause and effect. Below you will find some useful links describing the different types of variables.

• "What are independent and dependent variables" NCES
• [VIDEO] Biology: Independent vs. Dependent Variables (Nucleus Medical Media) Video explaining independent and dependent variables, with examples.

Resource Links

• What is and How to Write a Good Hypothesis in Research? (Elsevier)
• Hypothesis brochure from Penn State/Berks

• << Previous: Step 2: RESEARCH
• Next: Step 4: EXPERIMENT >>
• Last Updated: Aug 2, 2024 3:45 PM
• URL: https://harford.libguides.com/scientific_method

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Biology archive

Course: biology archive   >   unit 1, the scientific method.

• Controlled experiments
• The scientific method and experimental design

Introduction

• Make an observation.
• Ask a question.
• Form a hypothesis , or testable explanation.
• Make a prediction based on the hypothesis.
• Test the prediction.
• Iterate: use the results to make new hypotheses or predictions.

Scientific method example: Failure to toast

1. make an observation., 2. ask a question., 3. propose a hypothesis., 4. make predictions., 5. test the predictions..

• If the toaster does toast, then the hypothesis is supported—likely correct.
• If the toaster doesn't toast, then the hypothesis is not supported—likely wrong.

Logical possibility

Practical possibility, building a body of evidence, 6. iterate..

• If the hypothesis was supported, we might do additional tests to confirm it, or revise it to be more specific. For instance, we might investigate why the outlet is broken.
• If the hypothesis was not supported, we would come up with a new hypothesis. For instance, the next hypothesis might be that there's a broken wire in the toaster.

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On the scope of scientific hypotheses

William hedley thompson.

1 Department of Applied Information Technology, University of Gothenburg, Gothenburg, Sweden

2 Institute of Neuroscience and Physiology, Sahlgrenska Academy, University of Gothenburg, Gothenburg, Sweden

3 Department of Pedagogical, Curricular and Professional Studies, Faculty of Education, University of Gothenburg, Gothenburg, Sweden

4 Department of Clinical Neuroscience, Karolinska Institutet, Stockholm, Sweden

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Hypotheses are frequently the starting point when undertaking the empirical portion of the scientific process. They state something that the scientific process will attempt to evaluate, corroborate, verify or falsify. Their purpose is to guide the types of data we collect, analyses we conduct, and inferences we would like to make. Over the last decade, metascience has advocated for hypotheses being in preregistrations or registered reports, but how to formulate these hypotheses has received less attention. Here, we argue that hypotheses can vary in specificity along at least three independent dimensions: the relationship, the variables, and the pipeline. Together, these dimensions form the scope of the hypothesis. We demonstrate how narrowing the scope of a hypothesis in any of these three ways reduces the hypothesis space and that this reduction is a type of novelty. Finally, we discuss how this formulation of hypotheses can guide researchers to formulate the appropriate scope for their hypotheses and should aim for neither too broad nor too narrow a scope. This framework can guide hypothesis-makers when formulating their hypotheses by helping clarify what is being tested, chaining results to previous known findings, and demarcating what is explicitly tested in the hypothesis.

1.  Introduction

Hypotheses are an important part of the scientific process. However, surprisingly little attention is given to hypothesis-making compared to other skills in the scientist's skillset within current discussions aimed at improving scientific practice. Perhaps this lack of emphasis is because the formulation of the hypothesis is often considered less relevant, as it is ultimately the scientific process that will eventually decide the veracity of the hypothesis. However, there are more hypotheses than scientific studies as selection occurs at various stages: from funder selection and researcher's interests. So which hypotheses are worthwhile to pursue? Which hypotheses are the most effective or pragmatic for extending or enhancing our collective knowledge? We consider the answer to these questions by discussing how broad or narrow a hypothesis can or should be (i.e. its scope).

We begin by considering that the two statements below are both hypotheses and vary in scope:

• H 1 : For every 1 mg decrease of x , y will increase by, on average, 2.5 points.
• H 2 : Changes in x 1 or x 2 correlate with y levels in some way.

Clearly, the specificity of the two hypotheses is very different. H 1 states a precise relationship between two variables ( x and y ), while H 2 specifies a vaguer relationship and does not specify which variables will show the relationship. However, they are both still hypotheses about how x and y relate to each other. This claim of various degrees of the broadness of hypotheses is, in and of itself, not novel. In Epistemetrics, Rescher [ 1 ], while drawing upon the physicist Duhem's work, develops what he calls Duhem's Law. This law considers a trade-off between certainty or precision in statements about physics when evaluating them. Duhem's Law states that narrower hypotheses, such as H 1 above, are more precise but less likely to be evaluated as true than broader ones, such as H 2 above. Similarly, Popper, when discussing theories, describes the reverse relationship between content and probability of a theory being true, i.e. with increased content, there is a decrease in probability and vice versa [ 2 ]. Here we will argue that it is important that both H 1 and H 2 are still valid scientific hypotheses, and their appropriateness depends on certain scientific questions.

The question of hypothesis scope is relevant since there are multiple recent prescriptions to improve science, ranging from topics about preregistrations [ 3 ], registered reports [ 4 ], open science [ 5 ], standardization [ 6 ], generalizability [ 7 ], multiverse analyses [ 8 ], dataset reuse [ 9 ] and general questionable research practices [ 10 ]. Within each of these issues, there are arguments to demarcate between confirmatory and exploratory research or normative prescriptions about how science should be done (e.g. science is ‘bad’ or ‘worse’ if code/data are not open). Despite all these discussions and improvements, much can still be done to improve hypothesis-making. A recent evaluation of preregistered studies in psychology found that over half excluded the preregistered hypotheses [ 11 ]. Further, evaluations of hypotheses in ecology showed that most hypotheses are not explicitly stated [ 12 , 13 ]. Other research has shown that obfuscated hypotheses are more prevalent in retracted research [ 14 ]. There have been recommendations for simpler hypotheses in psychology to avoid misinterpretations and misspecifications [ 15 ]. Finally, several evaluations of preregistration practices have found that a significant proportion of articles do not abide by their stated hypothesis or add additional hypotheses [ 11 , 16 – 18 ]. In sum, while multiple efforts exist to improve scientific practice, our hypothesis-making could improve.

One of our intentions is to provide hypothesis-makers with tools to assist them when making hypotheses. We consider this useful and timely as, with preregistrations becoming more frequent, the hypothesis-making process is now open and explicit . However, preregistrations are difficult to write [ 19 ], and preregistered articles can change or omit hypotheses [ 11 ] or they are vague and certain degrees of freedom hard to control for [ 16 – 18 ]. One suggestion has been to do less confirmatory research [ 7 , 20 ]. While we agree that all research does not need to be confirmatory, we also believe that not all preregistrations of confirmatory work must test narrow hypotheses. We think there is a possible point of confusion that the specificity in preregistrations, where researcher degrees of freedom should be stated, necessitates the requirement that the hypothesis be narrow. Our belief that this confusion is occurring is supported by the study Akker et al . [ 11 ] where they found that 18% of published psychology studies changed their preregistered hypothesis (e.g. its direction), and 60% of studies selectively reported hypotheses in some way. It is along these lines that we feel the framework below can be useful to help formulate appropriate hypotheses to mitigate these identified issues.

We consider this article to be a discussion of the researcher's different choices when formulating hypotheses and to help link hypotheses over time. Here we aim to deconstruct what aspects there are in the hypothesis about their specificity. Throughout this article, we intend to be neutral to many different philosophies of science relating to the scientific method (i.e. how one determines the veracity of a hypothesis). Our idea of neutrality here is that whether a researcher adheres to falsification, verification, pragmatism, or some other philosophy of science, then this framework can be used when formulating hypotheses. 1

The framework this article advocates for is that there are (at least) three dimensions that hypotheses vary along regarding their narrowness and broadness: the selection of relationships, variables, and pipelines. We believe this discussion is fruitful for the current debate regarding normative practices as some positions make, sometimes implicit, commitments about which set of hypotheses the scientific community ought to consider good or permissible. We proceed by outlining a working definition of ‘scientific hypothesis' and then discuss how it relates to theory. Then, we justify how hypotheses can vary along the three dimensions. Using this framework, we then discuss the scopes in relation to appropriate hypothesis-making and an argument about what constitutes a scientifically novel hypothesis. We end the article with practical advice for researchers who wish to use this framework.

2.  The scientific hypothesis

In this section, we will describe a functional and descriptive role regarding how scientists use hypotheses. Jeong & Kwon [ 21 ] investigated and summarized the different uses the concept of ‘hypothesis’ had in philosophical and scientific texts. They identified five meanings: assumption, tentative explanation, tentative cause, tentative law, and prediction. Jeong & Kwon [ 21 ] further found that researchers in science and philosophy used all the different definitions of hypotheses, although there was some variance in frequency between fields. Here we see, descriptively , that the way researchers use the word ‘hypothesis’ is diverse and has a wide range in specificity and function. However, whichever meaning a hypothesis has, it aims to be true, adequate, accurate or useful in some way.

Not all hypotheses are ‘scientific hypotheses'. For example, consider the detective trying to solve a crime and hypothesizing about the perpetrator. Such a hypothesis still aims to be true and is a tentative explanation but differs from the scientific hypothesis. The difference is that the researcher, unlike the detective, evaluates the hypothesis with the scientific method and submits the work for evaluation by the scientific community. Thus a scientific hypothesis entails a commitment to evaluate the statement with the scientific process . 2 Additionally, other types of hypotheses can exist. As discussed in more detail below, scientific theories generate not only scientific hypotheses but also contain auxiliary hypotheses. The latter refers to additional assumptions considered to be true and not explicitly evaluated. 3

Next, the scientific hypothesis is generally made antecedent to the evaluation. This does not necessitate that the event (e.g. in archaeology) or the data collection (e.g. with open data reuse) must be collected before the hypothesis is made, but that the evaluation of the hypothesis cannot happen before its formulation. This claim state does deny the utility of exploratory hypothesis testing of post hoc hypotheses (see [ 25 ]). However, previous results and exploration can generate new hypotheses (e.g. via abduction [ 22 , 26 – 28 ], which is the process of creating hypotheses from evidence), which is an important part of science [ 29 – 32 ], but crucially, while these hypotheses are important and can be the conclusion of exploratory work, they have yet to be evaluated (by whichever method of choice). Hence, they still conform to the antecedency requirement. A further way to justify the antecedency is seen in the practice of formulating a post hoc hypothesis, and considering it to have been evaluated is seen as a questionable research practice (known as ‘hypotheses after results are known’ or HARKing [ 33 ]). 4

While there is a varying range of specificity, is the hypothesis a critical part of all scientific work, or is it reserved for some subset of investigations? There are different opinions regarding this. Glass and Hall, for example, argue that the term only refers to falsifiable research, and model-based research uses verification [ 36 ]. However, this opinion does not appear to be the consensus. Osimo and Rumiati argue that any model based on or using data is never wholly free from hypotheses, as hypotheses can, even implicitly, infiltrate the data collection [ 37 ]. For our definition, we will consider hypotheses that can be involved in different forms of scientific evaluation (i.e. not just falsification), but we do not exclude the possibility of hypothesis-free scientific work.

Finally, there is a debate about whether theories or hypotheses should be linguistic or formal [ 38 – 40 ]. Neither side in this debate argues that verbal or formal hypotheses are not possible, but instead, they discuss normative practices. Thus, for our definition, both linguistic and formal hypotheses are considered viable.

Considering the above discussion, let us summarize the scientific process and the scientific hypothesis: a hypothesis guides what type of data are sampled and what analysis will be done. With the new observations, evidence is analysed or quantified in some way (often using inferential statistics) to judge the hypothesis's truth value, utility, credibility, or likelihood. The following working definition captures the above:

• Scientific hypothesis : an implicit or explicit statement that can be verbal or formal. The hypothesis makes a statement about some natural phenomena (via an assumption, explanation, cause, law or prediction). The scientific hypothesis is made antecedent to performing a scientific process where there is a commitment to evaluate it.

For simplicity, we will only use the term ‘hypothesis’ for ‘scientific hypothesis' to refer to the above definition for the rest of the article except when it is necessary to distinguish between other types of hypotheses. Finally, this definition could further be restrained in multiple ways (e.g. only explicit hypotheses are allowed, or assumptions are never hypotheses). However, if the definition is more (or less) restrictive, it has little implication for the argument below.

3.  The hypothesis, theory and auxiliary assumptions

While we have a definition of the scientific hypothesis, we have yet to link it with how it relates to scientific theory, where there is frequently some interconnection (i.e. a hypothesis tests a scientific theory). Generally, for this paper, we believe our argument applies regardless of how scientific theory is defined. Further, some research lacks theory, sometimes called convenience or atheoretical studies [ 41 ]. Here a hypothesis can be made without a wider theory—and our framework fits here too. However, since many consider hypotheses to be defined or deducible from scientific theory, there is an important connection between the two. Therefore, we will briefly clarify how hypotheses relate to common formulations of scientific theory.

A scientific theory is generally a set of axioms or statements about some objects, properties and their relations relating to some phenomena. Hypotheses can often be deduced from the theory. Additionally, a theory has boundary conditions. The boundary conditions specify the domain of the theory stating under what conditions it applies (e.g. all things with a central neural system, humans, women, university teachers) [ 42 ]. Boundary conditions of a theory will consequently limit all hypotheses deduced from the theory. For example, with a boundary condition ‘applies to all humans’, then the subsequent hypotheses deduced from the theory are limited to being about humans. While this limitation of the hypothesis by the theory's boundary condition exists, all the considerations about a hypothesis scope detailed below still apply within the boundary conditions. Finally, it is also possible (depending on the definition of scientific theory) for a hypothesis to test the same theory under different boundary conditions. 5

The final consideration relating scientific theory to scientific hypotheses is auxiliary hypotheses. These hypotheses are theories or assumptions that are considered true simultaneously with the theory. Most philosophies of science from Popper's background knowledge [ 24 ], Kuhn's paradigms during normal science [ 44 ], and Laktos' protective belt [ 45 ] all have their own versions of this auxiliary or background information that is required for the hypothesis to test the theory. For example, Meelh [ 46 ] auxiliary theories/assumptions are needed to go from theoretical terms to empirical terms (e.g. neural activity can be inferred from blood oxygenation in fMRI research or reaction time to an indicator of cognition) and auxiliary theories about instruments (e.g. the experimental apparatus works as intended) and more (see also Other approaches to categorizing hypotheses below). As noted in the previous section, there is a difference between these auxiliary hypotheses, regardless of their definition, and the scientific hypothesis defined above. Recall that our definition of the scientific hypothesis included a commitment to evaluate it. There are no such commitments with auxiliary hypotheses, but rather they are assumed to be correct to test the theory adequately. This distinction proves to be important as auxiliary hypotheses are still part of testing a theory but are separate from the hypothesis to be evaluated (discussed in more detail below).

4.  The scope of hypotheses

In the scientific hypothesis section, we defined the hypothesis and discussed how it relates back to the theory. In this section, we want to defend two claims about hypotheses:

• (A1) Hypotheses can have different scopes . Some hypotheses are narrower in their formulation, and some are broader.
• (A2) The scope of hypotheses can vary along three dimensions relating to relationship selection , variable selection , and pipeline selection .

A1 may seem obvious, but it is important to establish what is meant by narrower and broader scope. When a hypothesis is very narrow, it is specific. For example, it might be specific about the type of relationship between some variables. In figure 1 , we make four different statements regarding the relationship between x and y . The narrowest hypothesis here states ‘there is a positive linear relationship with a magnitude of 0.5 between x and y ’ ( figure 1 a ), and the broadest hypothesis states ‘there is a relationship between x and y ’ ( figure 1 d ). Note that many other hypotheses are possible that are not included in this example (such as there being no relationship).

Examples of narrow and broad hypotheses between x and y . Circles indicate a set of possible relationships with varying slopes that can pivot or bend.

We see that the narrowest of these hypotheses claims a type of relationship (linear), a direction of the relationship (positive) and a magnitude of the relationship (0.5). As the hypothesis becomes broader, the specific magnitude disappears ( figure 1 b ), the relationship has additional options than just being linear ( figure 1 c ), and finally, the direction of the relationship disappears. Crucially, all the examples in figure 1 can meet the above definition of scientific hypotheses. They are all statements that can be evaluated with the same scientific method. There is a difference between these statements, though— they differ in the scope of the hypothesis . Here we have justified A1.

Within this framework, when we discuss whether a hypothesis is narrower or broader in scope, this is a relation between two hypotheses where one is a subset of the other. This means that if H 1 is narrower than H 2 , and if H 1 is true, then H 2 is also true. This can be seen in figure 1 a–d . Suppose figure 1 a , the narrowest of all the hypotheses, is true. In that case, all the other broader statements are also true (i.e. a linear correlation of 0.5 necessarily entails that there is also a positive linear correlation, a linear correlation, and some relationship). While this property may appear trivial, it entails that it is only possible to directly compare the hypothesis scope between two hypotheses (i.e. their broadness or narrowness) where one is the subset of the other. 6

4.1. Sets, disjunctions and conjunctions of elements

The above restraint defines the scope as relations between sets. This property helps formalize the framework of this article. Below, when we discuss the different dimensions that can impact the scope, these become represented as a set. Each set contains elements. Each element is a permissible situation that allows the hypothesis to be accepted. We denote elements as lower case with italics (e.g. e 1 , e 2 , e 3 ) and sets as bold upper case (e.g. S ). Each of the three different dimensions discussed below will be formalized as sets, while the total number of elements specifies their scope.

Let us reconsider the above restraint about comparing hypotheses as narrower or broader. This can be formally shown if:

• e 1 , e 2 , e 3 are elements of S 1 ; and
• e 1 and e 2 are elements of S 2 ,

then S 2 is narrower than S 1 .

Each element represents specific propositions that, if corroborated, would support the hypothesis. Returning to figure 1 a , b , the following statements apply to both:

• ‘There is a positive linear relationship between x and y with a slope of 0.5’.

Whereas the following two apply to figure 1 b but not figure 1 a :

• ‘There is a positive linear relationship between x and y with a slope of 0.4’ ( figure 1 b ).
• ‘There is a positive linear relationship between x and y with a slope of 0.3’ ( figure 1 b ).

Figure 1 b allows for a considerably larger number of permissible situations (which is obvious as it allows for any positive linear relationship). When formulating the hypothesis in figure 1 b , we do not need to specify every single one of these permissible relationships. We can simply specify all possible positive slopes, which entails the set of permissible elements it includes.

That broader hypotheses have more elements in their sets entails some important properties. When we say S contains the elements e 1 , e 2 , and e 3 , the hypothesis is corroborated if e 1 or e 2 or e 3 is the case. This means that the set requires only one of the elements to be corroborated for the hypothesis to be considered correct (i.e. the positive linear relationship needs to be 0.3 or 0.4 or 0.5). Contrastingly, we will later see cases when conjunctions of elements occur (i.e. both e 1 and e 2 are the case). When a conjunction occurs, in this formulation, the conjunction itself becomes an element in the set (i.e. ‘ e 1 and e 2 ’ is a single element). Figure 2 illustrates how ‘ e 1 and e 2 ’ is narrower than ‘ e 1 ’, and ‘ e 1 ’ is narrower than ‘ e 1 or e 2 ’. 7 This property relating to the conjunction being narrower than individual elements is explained in more detail in the pipeline selection section below.

Scope as sets. Left : four different sets (grey, red, blue and purple) showing different elements which they contain. Right : a list of each colour explaining which set is a subset of the other (thereby being ‘narrower’).

4.2. Relationship selection

We move to A2, which is to show the different dimensions that a hypothesis scope can vary along. We have already seen an example of the first dimension of a hypothesis in figure 1 , the relationship selection . Let R denote the set of all possible configurations of relationships that are permissible for the hypothesis to be considered true. For example, in the narrowest formulation above, there was one allowed relationship for the hypothesis to be true. Consequently, the size of R (denoted | R |) is one. As discussed above, in the second narrowest formulation ( figure 1 b ), R has more possible relationships where it can still be considered true:

• r 1 = ‘a positive linear relationship of 0.1’
• r 2 = ‘a positive linear relationship of 0.2’
• r 3 = ‘a positive linear relationship of 0.3’.

Additionally, even broader hypotheses will be compatible with more types of relationships. In figure 1 c , d , nonlinear and negative relationships are also possible relationships included in R . For this broader statement to be affirmed, more elements are possible to be true. Thus if | R | is greater (i.e. contains more possible configurations for the hypothesis to be true), then the hypothesis is broader. Thus, the scope of relating to the relationship selection is specified by | R |. Finally, if |R H1 | > |R H2 | , then H 1 is broader than H 2 regarding the relationship selection.

Figure 1 is an example of the relationship narrowing. That the relationship became linear is only an example and does not necessitate a linear relationship or that this scope refers only to correlations. An alternative example of a relationship scope is a broad hypothesis where there is no knowledge about the distribution of some data. In such situations, one may assume a uniform relationship or a Cauchy distribution centred at zero. Over time the specific distribution can be hypothesized. Thereafter, the various parameters of the distribution can be hypothesized. At each step, the hypothesis of the distribution gets further specified to narrower formulations where a smaller set of possible relationships are included (see [ 47 , 48 ] for a more in-depth discussion about how specific priors relate to more narrow tests). Finally, while figure 1 was used to illustrate the point of increasingly narrow relationship hypotheses, it is more likely to expect the narrowest relationship, within fields such as psychology, to have considerable uncertainty and be formulated with confidence or credible intervals (i.e. we will rarely reach point estimates).

4.3. Variable selection

We have demonstrated that relationship selection can affect the scope of a hypothesis. Additionally, at least two other dimensions can affect the scope of a hypothesis: variable selection and pipeline selection . The variable selection in figure 1 was a single bivariate relationship (e.g. x 's relationship with y ). However, it is not always the case that we know which variables will be involved. For example, in neuroimaging, we can be confident that one or more brain regions will be processing some information following a stimulus. Still, we might not be sure which brain region(s) this will be. Consequently, our hypothesis becomes broader because we have selected more variables. The relationship selection may be identical for each chosen variable, but the variable selection becomes broader. We can consider the following three hypotheses to be increasing in their scope:

• H 1 : x relates to y with relationship R .
• H 2 : x 1 or x 2 relates to y with relationship R .
• H 3 : x 1 or x 2 or x 3 relates to y with relationship R .

For H 1 –H 3 above, we assume that R is the same. Further, we assume that there is no interaction between these variables.

In the above examples, we have multiple x ( x 1 , x 2 , x 3 , … , x n ). Again, we can symbolize the variable selection as a non-empty set XY , containing either a single variable or many variables. Our motivation for designating it XY is that the variable selection can include multiple possibilities for both the independent variable ( x ) and the dependent variable ( y ). Like with relationship selection, we can quantify the broadness between two hypotheses with the size of the set XY . Consequently, | XY | denotes the total scope concerning variable selection. Thus, in the examples above | XY H1 | < | XY H2 | < | XY H3 |. Like with relationship selection, hypotheses that vary in | XY | still meet the definition of a hypothesis. 8

An obvious concern for many is that a broader XY is much easier to evaluate as correct. Generally, when | XY 1 | > | XY 2 |, there is a greater chance of spurious correlations when evaluating XY 1 . This concern is an issue relating to the evaluation of hypotheses (e.g. applying statistics to the evaluation), which will require additional assumptions relating to how to evaluate the hypotheses. Strategies to deal with this apply some correction or penalization for multiple statistical testing [ 49 ] or partial pooling and regularizing priors [ 50 , 51 ]. These strategies aim to evaluate a broader variable selection ( x 1 or x 2 ) on equal or similar terms to a narrow variable selection ( x 1 ).

4.4. Pipeline selection

Scientific studies require decisions about how to perform the analysis. This scope considers transformations applied to the raw data ( XY raw ) to achieve some derivative ( XY ). These decisions can also involve selection procedures that drop observations deemed unreliable, standardizing, correcting confounding variables, or different philosophies. We can call the array of decisions and transformations used as the pipeline . A hypothesis varies in the number of pipelines:

• H 1 : XY has a relationship(s) R with pipeline p 1 .
• H 2 : XY has a relationship(s) R with pipeline p 1 or pipeline p 2 .
• H 3 : XY has a relationship(s) R with pipeline p 1 or pipeline p 2 , or pipeline p 3 .

Importantly, the pipeline here considers decisions regarding how the hypothesis shapes the data collection and transformation. We do not consider this to include decisions made regarding the assumptions relating to the statistical inference as those relate to operationalizing the evaluation of the hypothesis and not part of the hypothesis being evaluated (these assumptions are like auxiliary hypotheses, which are assumed to be true but not explicitly evaluated).

Like with variable selection ( XY ) and relationship selection ( R ), we can see that pipelines impact the scope of hypotheses. Again, we can symbolize the pipeline selection with a set P . As previously, | P | will denote the dimension of the pipeline selection. In the case of pipeline selection, we are testing the same variables, looking for the same relationship, but processing the variables or relationships with different pipelines to evaluate the relationship. Consequently, | P H1 | < | P H2 | < | P H3 |.

These issues regarding pipelines have received attention as the ‘garden of forking paths' [ 52 ]. Here, there are calls for researchers to ensure that their entire pipeline has been specified. Additionally, recent work has highlighted the diversity of results based on multiple analytical pipelines [ 53 , 54 ]. These results are often considered a concern, leading to calls that results should be pipeline resistant.

The wish for pipeline-resistant methods entails that hypotheses, in their narrowest form, are possible for all pipelines. Consequently, a narrower formulation will entail that this should not impact the hypothesis regardless of which pipeline is chosen. Thus the conjunction of pipelines is narrower than single pipelines. Consider the following three scenarios:

• H 3 : XY has a relationship(s) R with pipeline p 1 and pipeline p 2 .

In this instance, since H 1 is always true if H 3 is true, thus H 3 is a narrower formulation than H 1 . Consequently, | P H3 | < | P H1 | < | P H2 |. Decreasing the scope of the pipeline dimension also entails the increase in conjunction of pipelines (i.e. creating pipeline-resistant methods) rather than just the reduction of disjunctional statements.

4.5. Combining the dimensions

In summary, we then have three different dimensions that independently affect the scope of the hypothesis. We have demonstrated the following general claim regarding hypotheses:

• The variables XY have a relationship R with pipeline P .

And that the broadness and narrowness of a hypothesis depend on how large the three sets XY , R and P are. With this formulation, we can conclude that hypotheses have a scope that can be determined with a 3-tuple argument of (| R |, | XY |, | P |).

While hypotheses can be formulated along these three dimensions and generally aim to be reduced, it does not entail that these dimensions behave identically. For example, the relationship dimensions aim to reduce the number of elements as far as possible (e.g. to an interval). Contrastingly, for both variables and pipeline, the narrower hypothesis can reduce to single variables/pipelines or become narrower still and become conjunctions where all variables/pipelines need to corroborate the hypothesis (i.e. regardless of which method one follows, the hypothesis is correct).

5.  Additional possible dimensions

No commitment is being made about the exhaustive nature of there only being three dimensions that specify the hypothesis scope. Other dimensions may exist that specify the scope of a hypothesis. For example, one might consider the pipeline dimension as two different dimensions. The first would consider the experimental pipeline dimension regarding all variables relating to the experimental setup to collect data, and the latter would be the analytical pipeline dimension regarding the data analysis of any given data snapshot. Another possible dimension is adding the number of situations or contexts under which the hypothesis is valid. For example, any restraint such as ‘in a vacuum’, ‘under the speed of light’, or ‘in healthy human adults' could be considered an additional dimension of the hypothesis. There is no objection to whether these should be additional dimensions of the hypothesis. However, as stated above, these usually follow from the boundary conditions of the theory.

6.  Specifying the scope versus assumptions

We envision that this framework can help hypothesis-makers formulate hypotheses (in research plans, registered reports, preregistrations etc.). Further, using this framework while formulating hypotheses can help distinguish between auxiliary hypotheses and parts of the scientific hypothesis being tested. When writing preregistrations, it can frequently occur that some step in the method has two alternatives (e.g. a preprocessing step), and there is not yet reason to choose one over the other, and the researcher needs to make a decision. These following scenarios are possible:

• 1. Narrow pipeline scope . The researcher evaluates the hypothesis with both pipeline variables (i.e. H holds for both p 1 and p 2 where p 1 and p 2 can be substituted with each other in the pipeline).
• 2. Broad pipeline scope. The researcher evaluates the hypothesis with both pipeline variables, and only one needs to be correct (i.e. H holds for either p 1 or p 2 where p 1 and p 2 can be substituted with each other in the pipeline). The result of this experiment may help motivate choosing either p 1 or p 2 in future studies.
• 3. Auxiliary hypothesis. Based on some reason (e.g. convention), the researcher assumes p 1 and evaluates H assuming p 1 is true.

Here we see that the same pipeline step can be part of either the auxiliary hypotheses or the pipeline scope. This distinction is important because if (3) is chosen, the decision becomes an assumption that is not explicitly tested by the hypothesis. Consequently, a researcher confident in the hypothesis may state that the auxiliary hypothesis p 1 was incorrect, and they should retest their hypothesis using different assumptions. In the cases where this decision is part of the pipeline scope, the hypothesis is intertwined with this decision, removing the eventual wiggle-room to reject auxiliary hypotheses that were assumed. Furthermore, starting with broader pipeline hypotheses that gradually narrow down can lead to a more well-motivated protocol for approaching the problem. Thus, this framework can help researchers while writing their hypotheses in, for example, preregistrations because they can consider when they are committing to a decision, assuming it, or when they should perhaps test a broader hypothesis with multiple possible options (discussed in more detail in §11 below).

7.  The reduction of scope in hypothesis space

Having established that different scopes of a hypothesis are possible, we now consider how the hypotheses change over time. In this section, we consider how the scope of the hypothesis develops ideally within science.

Consider a new research question. A large number of hypotheses are possible. Let us call this set of all possible hypotheses the hypothesis space . Hypotheses formulated within this space can be narrower or broader based on the dimensions discussed previously ( figure 3 ).

Example of hypothesis space. The hypothesis scope is expressed as cuboids in three dimensions (relationship ( R ), variable ( XY ), pipeline ( P )). The hypothesis space is the entire possible space within the three dimensions. Three hypotheses are shown in the hypothesis space (H 1 , H 2 , H 3 ). H 2 and H 3 are subsets of H 1 .

After the evaluation of the hypothesis with the scientific process, the hypothesis will be accepted or rejected. 9 The evaluation could be done through falsification or via verification, depending on the philosophy of science commitments. Thereafter, other narrower formulations of the hypothesis can be formulated by reducing the relationship, variable or pipeline scope. If a narrower hypothesis is accepted, more specific details about the subject matter are known, or a theory has been refined in greater detail. A narrower hypothesis will entail a more specific relationship, variable or pipeline detailed in the hypothesis. Consequently, hypotheses linked to each other in this way will become narrower over time along one or more dimensions. Importantly, considering that the conjunction of elements is narrower than single elements for pipelines and variables, this process of narrower hypotheses will lead to more general hypotheses (i.e. they have to be applied in all conditions and yield less flexibility when they do not apply). 10

Considering that the scopes of hypotheses were defined as sets above, some properties can be deduced from this framework about how narrower hypotheses relate to broader hypotheses. Let us consider three hypotheses (H 1 , H 2 , and H 3 ; figure 3 ). H 2 and H 3 are non-overlapping subsets of H 1 . Thus H 2 and H 3 are both narrower in scope than H 1 . Thus the following is correct:

• P1: If H 1 is false, then H 2 is false, and H 2 does not need to be evaluated.
• P2: If H 2 is true, then the broader H 1 is true, and H 1 does not need to be evaluated.
• P3: If H 1 is true and H 2 is false, some other hypothesis H 3 of similar scope to H 2 is possible.

For example, suppose H 1 is ‘there is a relationship between x and y ’, H 2 is ‘there is a positive relationship between x and y ’, and H 3 is ‘a negative relationship between x and y ’. In that case, it becomes apparent how each of these follows. 11 Logically, many deductions from set theory are possible but will not be explored here. Instead, we will discuss two additional consequences of hypothesis scopes: scientific novelty and applications for the researcher who formulates a hypothesis.

P1–P3 have been formulated as hypotheses being true or false. In practice, hypotheses are likely evaluated probabilistically (e.g. ‘H 1 is likely’ or ‘there is evidence in support of H 1 ’). In these cases, P1–P3 can be rephrased to account for this by substituting true/false with statements relating to evidence. For example, P2 could read: ‘If there is evidence in support of H 2 , then there is evidence in support of H 1 , and H 1 does not need to be evaluated’.

8.  Scientific novelty as the reduction of scope

Novelty is a key concept that repeatedly occurs in multiple aspects of the scientific enterprise, from funding to publishing [ 55 ]. Generally, scientific progress establishes novel results based on some new hypothesis. Consequently, the new hypothesis for the novel results must be narrower than previously established knowledge (i.e. the size of the scopes is reduced). Otherwise, the result is trivial and already known (see P2 above). Thus, scientific work is novel if the scientific process produces a result based on hypotheses with either a smaller | R |, | XY |, or | P | compared to previous work.

This framework of dimensions of the scope of a hypothesis helps to demarcate when a hypothesis and the subsequent result are novel. If previous studies have established evidence for R 1 (e.g. there is a positive relationship between x and y ), a hypothesis will be novel if and only if it is narrower than R 1 . Thus, if R 2 is narrower in scope than R 1 (i.e. | R 2 | < | R 1 |), R 2 is a novel hypothesis.

Consider the following example. Study 1 hypothesizes, ‘There is a positive relationship between x and y ’. It identifies a linear relationship of 0.6. Next, Study 2 hypothesizes, ‘There is a specific linear relationship between x and y that is 0.6’. Study 2 also identifies the relationship of 0.6. Since this was a narrower hypothesis, Study 2 is novel despite the same result. Frequently, researchers claim that they are the first to demonstrate a relationship. Being the first to demonstrate a relationship is not the final measure of novelty. Having a narrower hypothesis than previous researchers is a sign of novelty as it further reduces the hypothesis space.

Finally, it should be noted that novelty is not the only objective of scientific work. Other attributes, such as improving the certainty of a current hypothesis (e.g. through replications), should not be overlooked. Additional scientific explanations and improved theories are other aspects. Additionally, this definition of novelty relating to hypothesis scope does not exclude other types of novelty (e.g. new theories or paradigms).

9.  How broad should a hypothesis be?

Given the previous section, it is elusive to conclude that the hypothesis should be as narrow as possible as it entails maximal knowledge gain and scientific novelty when formulating hypotheses. Indeed, many who advocate for daring or risky tests seem to hold this opinion. For example, Meehl [ 46 ] argues that we should evaluate theories based on point (or interval) prediction, which would be compatible with very narrow versions of relationships. We do not necessarily think that this is the most fruitful approach. In this section, we argue that hypotheses should aim to be narrower than current knowledge , but too narrow may be problematic .

Let us consider the idea of confirmatory analyses. These studies will frequently keep the previous hypothesis scopes regarding P and XY but aim to become more specific regarding R (i.e. using the same method and the same variables to detect a more specific relationship). A very daring or narrow hypothesis is to minimize R to include the fewest possible relationships. However, it becomes apparent that simply pursuing specificness or daringness is insufficient for selecting relevant hypotheses. Consider a hypothetical scenario where a researcher believes virtual reality use leads people to overestimate the amount of exercise they have done. If unaware of previous studies on this project, an apt hypothesis is perhaps ‘increased virtual reality usage correlates with a less accuracy of reported exercise performed’ (i.e. R is broad). However, a more specific and more daring hypothesis would be to specify the relationship further. Thus, despite not knowing if there is a relationship at all, a more daring hypothesis could be: ‘for every 1 h of virtual reality usage, there will be, on average, a 0.5% decrease in the accuracy of reported exercise performed’ (i.e. R is narrow). We believe it would be better to establish the broader hypothesis in any scenario here for the first experiment. Otherwise, if we fail to confirm the more specific formulation, we could reformulate another equally narrow relative to the broader hypothesis. This process of tweaking a daring hypothesis could be pursued ad infinitum . Such a situation will neither quickly identify the true hypothesis nor effectively use limited research resources.

By first discounting a broader hypothesis that there is no relationship, it will automatically discard all more specific formulations of that relationship in the hypothesis space. Returning to figure 3 , it will be better to establish H 1 before attempting H 2 or H 3 to ensure the correct area in the hypothesis space is being investigated. To provide an analogy: when looking for a needle among hay, first identify which farm it is at, then which barn, then which haystack, then which part of the haystack it is at before we start picking up individual pieces of hay. Thus, it is preferable for both pragmatic and cost-of-resource reasons to formulate sufficiently broad hypotheses to navigate the hypothesis space effectively.

Conversely, formulating too broad a relationship scope in a hypothesis when we already have evidence for narrower scope would be superfluous research (unless the evidence has been called into question by, for example, not being replicated). If multiple studies have supported the hypothesis ‘there is a 20-fold decrease in mortality after taking some medication M’, it would be unnecessary to ask, ‘Does M have any effect?’.

Our conclusion is that the appropriate scope of a hypothesis, and its three dimensions, follow a Goldilocks-like principle where too broad is superfluous and not novel, while too narrow is unnecessary or wasteful. Considering the scope of one's hypothesis and how it relates to previous hypotheses' scopes ensures one is asking appropriate questions.

Finally, there has been a recent trend in psychology that hypotheses should be formal [ 38 , 56 – 60 ]. Formal theories are precise since they are mathematical formulations entailing that their interpretations are clear (non-ambiguous) compared to linguistic theories. However, this literature on formal theories often refers to ‘precise predictions’ and ‘risky testing’ while frequently referencing Meehl, who advocates for narrow hypotheses (e.g. [ 38 , 56 , 59 ]). While perhaps not intended by any of the proponents, one interpretation of some of these positions is that hypotheses derived from formal theories will be narrow hypotheses (i.e. the quality of being ‘precise’ can mean narrow hypotheses with risky tests and non-ambiguous interpretations simultaneously). However, the benefit from the clarity (non-ambiguity) that formal theories/hypotheses bring also applies to broad formal hypotheses as well. They can include explicit but formalized versions of uncertain relationships, multiple possible pipelines, and large sets of variables. For example, a broad formal hypothesis can contain a hyperparameter that controls which distribution the data fit (broad relationship scope), or a variable could represent a set of formalized explicit pipelines (broad pipeline scope) that will be tested. In each of these instances, it is possible to formalize non-ambiguous broad hypotheses from broad formal theories that do not yet have any justification for being overly narrow. In sum, our argumentation here stating that hypotheses should not be too narrow is not an argument against formal theories but rather that hypotheses (derived from formal theories) do not necessarily have to be narrow.

10.  Other approaches to categorizing hypotheses

The framework we present here is a way of categorizing hypotheses into (at least) three dimensions regarding the hypothesis scope, which we believe is accessible to researchers and help link scientific work over time while also trying to remain neutral with regard to a specific philosophy of science. Our proposal does not aim to be antagonistic or necessarily contradict other categorizing schemes—but we believe that our framework provides benefits.

One recent categorization scheme is the Theoretical (T), Auxiliary (A), Statistical (S) and Inferential (I) assumption model (together becoming the TASI model) [ 61 , 62 ]. Briefly, this model considers theory to generate theoretical hypotheses. To translate from theoretical unobservable terms (e.g. personality, anxiety, mass), auxiliary assumptions are needed to generate an empirical hypothesis. Statistical assumptions are often needed to test the empirical hypothesis (e.g. what is the distribution, is it skewed or not) [ 61 , 62 ]. Finally, additional inferential assumptions are needed to generalize to a larger population (e.g. was there a random and independent sampling from defined populations). The TASI model is insightful and helpful in highlighting the distance between a theory and the observation that would corroborate/contradict it. Part of its utility is to bring auxiliary hypotheses into the foreground, to improve comparisons between studies and improve theory-based interventions [ 63 , 64 ].

We do agree with the importance of being aware of or stating the auxiliary hypotheses, but there are some differences between the frameworks. First, the number of auxiliary assumptions in TASI can be several hundred [ 62 ], whereas our framework will consider some of them as part of the pipeline dimension. Consider the following four assumptions: ‘the inter-stimulus interval is between 2000 ms and 3000 ms', ‘the data will be z-transformed’, ‘subjects will perform correctly’, and ‘the measurements were valid’. According to the TASI model, all these will be classified similarly as auxiliary assumptions. Contrarily, within our framework, it is possible to consider the first two as part of the pipeline dimension and the latter two as auxiliary assumptions, and consequently, the first two become integrated as part of the hypothesis being tested and the latter two auxiliary assumptions. A second difference between the frameworks relates to non-theoretical studies (convenience, applied or atheoretical). Our framework allows for the possibility that the hypothesis space generated by theoretical and convenience studies can interact and inform each other within the same framework . Contrarily, in TASI, the theory assumptions no longer apply, and a different type of hypothesis model is needed; these assumptions must be replaced by another group of assumptions (where ‘substantive application assumptions' replace the T and the A, becoming SSI) [ 61 ]. Finally, part of our rationale for our framework is to be able to link and track hypotheses and hypothesis development together over time, so our classification scheme has different utility.

Another approach which has some similar utility to this framework is theory construction methodology (TCM) [ 57 ]. The similarity here is that TCM aims to be a practical guide to improve theory-making in psychology. It is an iterative process which relates theory, phenomena and data. Here hypotheses are not an explicit part of the model. However, what is designated as ‘proto theory’ could be considered a hypothesis in our framework as they are a product of abduction, shaping the theory space. Alternatively, what is deduced to evaluate the theory can also be considered a hypothesis. We consider both possible and that our framework can integrate with these two steps, especially since TCM does not have clear guidelines for how to do each step.

11.  From theory to practice: implementing this framework

We believe that many practising researchers can relate to many aspects of this framework. But, how can a researcher translate the above theoretical framework to their work? The utility of this framework lies in bringing these three scopes of a hypothesis together and explaining how each can be reduced. We believe researchers can use this framework to describe their current practices more clearly. Here we discuss how it can be helpful for researchers when formulating, planning, preregistering, and discussing the evaluation of their scientific hypotheses. These practical implications are brief, and future work can expand on the connection between the full interaction between hypothesis space and scope. Furthermore, both authors have the most experience in cognitive neuroscience, and some of the practical implications may revolve around this type of research and may not apply equally to other fields.

11.1. Helping to form hypotheses

Abduction, according to Peirce, is a hypothesis-making exercise [ 22 , 26 – 28 ]. Given some observations, a general testable explanation of the phenomena is formed. However, when making the hypothesis, this statement will have a scope (either explicitly or implicitly). Using our framework, the scope can become explicit. The hypothesis-maker can start with ‘The variables XY have a relationship R with pipeline P ’ as a scaffold to form the hypothesis. From here, the hypothesis-maker can ‘fill in the blanks’, explicitly adding each of the scopes. Thus, when making a hypothesis via abduction and using our framework, the hypothesis will have an explicit scope when it is made. By doing this, there is less chance that a formulated hypothesis is unclear, ambiguous, and needs amending at a later stage.

11.2. Assisting to clearly state hypotheses

A hypothesis is not just formulated but also communicated. Hypotheses are stated in funding applications, preregistrations, registered reports, and academic articles. Further, preregistered hypotheses are often omitted or changed in the final article [ 11 ], and hypotheses are not always explicitly stated in articles [ 12 ]. How can this framework help to make better hypotheses? Similar to the previous point, filling in the details of ‘The variables XY have a relationship R with pipeline P ’ is an explicit way to communicate the hypothesis. Thinking about each of these dimensions should entail an appropriate explicit scope and, hopefully, less variation between preregistered and reported hypotheses. The hypothesis does not need to be a single sentence, and details of XY and P will often be developed in the methods section of the text. However, using this template as a starting point can help ensure the hypothesis is stated, and the scope of all three dimensions has been communicated.

11.3. Helping to promote explicit and broad hypotheses instead of vague hypotheses

There is an important distinction between vague hypotheses and broad hypotheses, and this framework can help demarcate between them. A vague statement would be: ‘We will quantify depression in patients after treatment’. Here there is uncertainty relating to how the researcher will go about doing the experiment (i.e. how will depression be quantified?). However, a broad statement can be uncertain, but the uncertainty is part of the hypothesis: ‘Two different mood scales (S 1 or S 2 ) will be given to patients and test if only one (or both) changed after treatment’. This latter statement is transparently saying ‘S 1 or S 2 ’ is part of a broad hypothesis—the uncertainty is whether the two different scales are quantifying the same construct. We keep this uncertainty within the broad hypothesis, which will get evaluated, whereas a vague hypothesis has uncertainty as part of the interpretation of the hypothesis. This framework can be used when formulating hypotheses to help be broad (where needed) but not vague.

11.4. Which hypothesis should be chosen?

When considering the appropriate scope above, we argued for a Goldilocks-like principle of determining the hypothesis that is not too broad or too narrow. However, when writing, for example, a preregistration, how does one identify this sweet spot? There is no easy or definite universal answer to this question. However, one possible way is first to identify the XY , R , and P of previous hypotheses. From here, identify what a non-trivial step is to improve our knowledge of the research area. So, for example, could you be more specific about the exact nature of the relationship between the variables? Does the pipeline correspond to today's scientific standards, or were some suboptimal decisions made? Is there another population that you think the previous result also applies to? Do you think that maybe a more specific construct or subpopulation might explain the previous result? Could slightly different constructs (perhaps easier to quantify) be used to obtain a similar relationship? Are there even more constructs to which this relationship should apply simultaneously? Are you certain of the direction of the relationship? Answering affirmatively to any of these questions will likely make a hypothesis narrower and connect to previous research while being clear and explicit. Moreover, depending on the research question, answering any of these may be sufficiently narrow to be a non-trivial innovation. However, there are many other ways to make a hypothesis narrower than these guiding questions.

11.5. The confirmatory–exploratory continuum

Research is often dichotomized into confirmatory (testing a hypothesis) or exploratory (without a priori hypotheses). With this framework, researchers can consider how their research acts on some hypothesis space. Confirmatory and exploratory work has been defined in terms of how each interacts with the researcher's degrees of freedom (where confirmatory aims to reduce while exploratory utilizes them [ 30 ]). Both broad confirmatory and narrow exploratory research are possible using this definition and possible within this framework. How research interacts with the hypothesis space helps demarcate it. For example, if a hypothesis reduces the scope, it becomes more confirmatory, and trying to understand data given the current scope would be more exploratory work. This further could help demarcate when exploration is useful. Future theoretical work can detail how different types of research impact the hypothesis space in more detail.

11.6. Understanding when multiverse analyses are needed

Researchers writing a preregistration may face many degrees of freedom they have to choose from, and different researchers may motivate different choices. If, when writing such a preregistration, there appears to be little evidential support for certain degrees of freedom over others, the researcher is left with the option to either make more auxiliary assumptions or identify when an investigation into the pipeline scope is necessary by conducting a multiverse analysis that tests the impact of the different degrees of freedom on the result (see [ 8 ]). Thus, when applying this framework to explicitly state what pipeline variables are part of the hypothesis or an auxiliary assumption, the researcher can identify when it might be appropriate to conduct a multiverse analysis because they are having difficulty formulating hypotheses.

11.7. Describing novelty

Academic journals and research funders often ask for novelty, but the term ‘novelty’ can be vague and open to various interpretations [ 55 ]. This framework can be used to help justify the novelty of research. For example, consider a scenario where a previous study has established a psychological construct (e.g. well-being) that correlates with a certain outcome measure (e.g. long-term positive health outcomes). This framework can be used to explicitly justify novelty by (i) providing a more precise understanding of the relationship (e.g. linear or linear–plateau) or (ii) identifying more specific variables related to well-being or health outcomes. Stating how some research is novel is clearer than merely stating that the work is novel. This practice might even help journals and funders identify what type of novelty they would like to reward. In sum, this framework can help identify and articulate how research is novel.

11.8. Help to identify when standardization of pipelines is beneficial or problematic to a field

Many consider standardization in a field to be important for ensuring the comparability of results. Standardization of methods and tools entails that the pipeline P is identical (or at least very similar) across studies. However, in such cases, the standardized pipeline becomes an auxiliary assumption representing all possible pipelines. Therefore, while standardized pipelines have their benefits, this assumption becomes broader without validating (e.g. via multiverse analysis) which pipelines a standardized P represents. In summary, because this framework helps distinguish between auxiliary assumptions and explicit parts of the hypothesis and identifies when a multiverse analysis is needed, it can help determine when standardizations of pipelines are representative (narrower hypotheses) or assumptive (broader hypotheses).

12.  Conclusion

Here, we have argued that the scope of a hypothesis is made up of three dimensions: the relationship ( R ), variable ( XY ) and pipeline ( P ) selection. Along each of these dimensions, the scope can vary. Different types of scientific enterprises will often have hypotheses that vary the size of the scopes. We have argued that this focus on the scope of the hypothesis along these dimensions helps the hypothesis-maker formulate their hypotheses for preregistrations while also helping demarcate auxiliary hypotheses (assumed to be true) from the hypothesis (those being evaluated during the scientific process).

Hypotheses are an essential part of the scientific process. Considering what type of hypothesis is sufficient or relevant is an essential job of the researcher that we think has been overlooked. We hope this work promotes an understanding of what a hypothesis is and how its formulation and reduction in scope is an integral part of scientific progress. We hope it also helps clarify how broad hypotheses need not be vague or inappropriate.

Finally, we applied this idea of scopes to scientific progress and considered how to formulate an appropriate hypothesis. We have also listed several ways researchers can practically implement this framework today. However, there are other practicalities of this framework that future work should explore. For example, it could be used to differentiate and demarcate different scientific contributions (e.g. confirmatory studies, exploration studies, validation studies) with how their hypotheses interact with the different dimensions of the hypothesis space. Further, linking hypotheses over time within this framework can be a foundation for open hypothesis-making by promoting explicit links to previous work and detailing the reduction of the hypothesis space. This framework helps quantify the contribution to the hypothesis space of different studies and helps clarify what aspects of hypotheses can be relevant at different times.

Acknowledgements

We thank Filip Gedin, Kristoffer Sundberg, Jens Fust, and James Steele for valuable feedback on earlier versions of this article. We also thank Mark Rubin and an unnamed reviewer for valuable comments that have improved the article.

1 While this is our intention, we cannot claim that every theory has been accommodated.

2 Similar requirements of science being able to evaluate the hypothesis can be found in pragmatism [ 22 ], logical positivism [ 23 ] and falsification [ 24 ].

3 Although when making inferences about a failed evaluation of a scientific hypothesis it is possible, due to underdetermination, to reject the auxiliary hypothesis instead of rejecting the hypothesis. However, that rejection occurs at a later inference stage. The evaluation using the scientific method aims to test the scientific hypothesis, not the auxiliary assumptions.

4 Although some have argued that this practice is not as problematic or questionable (see [ 34 , 35 ]).

5 Alternatively, theories sometimes expand their boundary conditions. A theory that was previously about ‘humans' can be used with a more inclusive boundary condition. Thus it is possible for the hypothesis-maker to use a theory about humans (decision making) and expand it to fruit flies or plants (see [ 43 ]).

6 A similarity exists here with Popper, where he uses set theory in a similar way to compare theories (not hypotheses). Popper also discusses how theories with overlapping sets but neither is a subset are also comparable (see [ 24 , §§32–34]). We do not exclude this possibility but can require additional assumptions.

7 When this could be unclear, we place the element within quotation marks.

8 Here, we have assumed that there is no interaction between these variables in variable selection. If an interaction between x 1 and x 2 is hypothesized, this should be viewed as a different variable compared to ‘ x 1 or x 2 ’. The motivation behind this is because the hypothesis ‘ x 1 or x 2 ’ is not a superset of the interaction (i.e. ‘ x 1 or x 2 ’ is not necessarily true when the interaction is true). The interaction should, in this case, be considered a third variable (e.g. I( x 1 , x 2 )) and the hypothesis ‘ x 1 or x 2 or I( x 1 , x 2 )’ is broader than ‘ x 1 or x 2 ’.

9 Or possibly ambiguous or inconclusive.

10 This formulation of scope is compatible with different frameworks from the philosophy of science. For example, by narrowing the scope would in a Popperian terminology mean prohibiting more basic statements (thus a narrower hypothesis has a higher degree of falsifiability). The reduction of scope in the relational dimension would in Popperian terminology mean increase in precision (e.g. a circle is more precise than an ellipse since circles are a subset of possible ellipses), whereas reduction in variable selection and pipeline dimension would mean increase universality (e.g. ‘all heavenly bodies' is more universal than just ‘planets') [ 24 ]. For Meehl the reduction of the relationship dimension would amount to decreasing the relative tolerance of a theory to the Spielraum [ 46 ] .

11 If there is no relationship between x and y , we do not need to test if there is a positive relationship. If we know there is a positive relationship between x and y , we do not need to test if there is a relationship. If we know there is a relationship but there is not a positive relationship, then it is possible that they have a negative relationship.

Data accessibility

Declaration of ai use.

We have not used AI-assisted technologies in creating this article.

Authors' contributions

W.H.T.: conceptualization, investigation, writing—original draft, writing—review and editing; S.S.: investigation, writing—original draft, writing—review and editing.

Both authors gave final approval for publication and agreed to be held accountable for the work performed therein.

Conflict of interest declaration

We declare we have no competing interests.

We received no funding for this study.

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What is a Hypothesis?

Experimental Design

Today, students learned about the importance of experimental design. Starting with the steps of the Ruler Drop Experiment which we can use to test reaction times, students came up with their own hypotheses about what variables might affect people’s reaction times. Then they came up with their own experimental plans to test these hypotheses. Students learned that it is important that a good hypothesis makes a claim about the relationship between two variables, and that this relationship is specific and testable in a measurable way. Students also learned that only one variable—the independent variable—can differ between test groups. Finally, we talked about how it is important to have more than one test subject so that an average can be taken. Ask your student to test your reaction times!

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• Education Resources Information Center - Understanding Hypotheses, Predictions, Laws, and Theories
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hypothesis , something supposed or taken for granted, with the object of following out its consequences (Greek hypothesis , “a putting under,” the Latin equivalent being suppositio ).

In planning a course of action, one may consider various alternatives , working out each in detail. Although the word hypothesis is not typically used in this case, the procedure is virtually the same as that of an investigator of crime considering various suspects. Different methods may be used for deciding what the various alternatives may be, but what is fundamental is the consideration of a supposal as if it were true, without actually accepting it as true. One of the earliest uses of the word in this sense was in geometry . It is described by Plato in the Meno .

The most important modern use of a hypothesis is in relation to scientific investigation . A scientist is not merely concerned to accumulate such facts as can be discovered by observation: linkages must be discovered to connect those facts. An initial puzzle or problem provides the impetus , but clues must be used to ascertain which facts will help yield a solution. The best guide is a tentative hypothesis, which fits within the existing body of doctrine. It is so framed that, with its help, deductions can be made that under certain factual conditions (“initial conditions”) certain other facts would be found if the hypothesis were correct.

The concepts involved in the hypothesis need not themselves refer to observable objects. However, the initial conditions should be able to be observed or to be produced experimentally, and the deduced facts should be able to be observed. William Harvey ’s research on circulation in animals demonstrates how greatly experimental observation can be helped by a fruitful hypothesis. While a hypothesis can be partially confirmed by showing that what is deduced from it with certain initial conditions is actually found under those conditions, it cannot be completely proved in this way. What would have to be shown is that no other hypothesis would serve. Hence, in assessing the soundness of a hypothesis, stress is laid on the range and variety of facts that can be brought under its scope. Again, it is important that it should be capable of being linked systematically with hypotheses which have been found fertile in other fields.

If the predictions derived from the hypothesis are not found to be true, the hypothesis may have to be given up or modified. The fault may lie, however, in some other principle forming part of the body of accepted doctrine which has been utilized in deducing consequences from the hypothesis. It may also lie in the fact that other conditions, hitherto unobserved, are present beside the initial conditions, affecting the result. Thus the hypothesis may be kept, pending further examination of facts or some remodeling of principles. A good illustration of this is to be found in the history of the corpuscular and the undulatory hypotheses about light .

Definition of a Hypothesis

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A hypothesis is a prediction of what will be found at the outcome of a research project and is typically focused on the relationship between two different variables studied in the research. It is usually based on both theoretical expectations about how things work and already existing scientific evidence.

Within social science, a hypothesis can take two forms. It can predict that there is no relationship between two variables, in which case it is a null hypothesis . Or, it can predict the existence of a relationship between variables, which is known as an alternative hypothesis.

In either case, the variable that is thought to either affect or not affect the outcome is known as the independent variable, and the variable that is thought to either be affected or not is the dependent variable.

Researchers seek to determine whether or not their hypothesis, or hypotheses if they have more than one, will prove true. Sometimes they do, and sometimes they do not. Either way, the research is considered successful if one can conclude whether or not a hypothesis is true. 

Null Hypothesis

A researcher has a null hypothesis when she or he believes, based on theory and existing scientific evidence, that there will not be a relationship between two variables. For example, when examining what factors influence a person's highest level of education within the U.S., a researcher might expect that place of birth, number of siblings, and religion would not have an impact on the level of education. This would mean the researcher has stated three null hypotheses.

Alternative Hypothesis

Taking the same example, a researcher might expect that the economic class and educational attainment of one's parents, and the race of the person in question are likely to have an effect on one's educational attainment. Existing evidence and social theories that recognize the connections between wealth and cultural resources , and how race affects access to rights and resources in the U.S. , would suggest that both economic class and educational attainment of the one's parents would have a positive effect on educational attainment. In this case, economic class and educational attainment of one's parents are independent variables, and one's educational attainment is the dependent variable—it is hypothesized to be dependent on the other two.

Conversely, an informed researcher would expect that being a race other than white in the U.S. is likely to have a negative impact on a person's educational attainment. This would be characterized as a negative relationship, wherein being a person of color has a negative effect on one's educational attainment. In reality, this hypothesis proves true, with the exception of Asian Americans , who go to college at a higher rate than whites do. However, Blacks and Hispanics and Latinos are far less likely than whites and Asian Americans to go to college.

Formulating a Hypothesis

Formulating a hypothesis can take place at the very beginning of a research project , or after a bit of research has already been done. Sometimes a researcher knows right from the start which variables she is interested in studying, and she may already have a hunch about their relationships. Other times, a researcher may have an interest in ​a particular topic, trend, or phenomenon, but he may not know enough about it to identify variables or formulate a hypothesis.

Whenever a hypothesis is formulated, the most important thing is to be precise about what one's variables are, what the nature of the relationship between them might be, and how one can go about conducting a study of them.

Updated by Nicki Lisa Cole, Ph.D

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What Is a Hypothesis and How Do I Write One?

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Think about something strange and unexplainable in your life. Maybe you get a headache right before it rains, or maybe you think your favorite sports team wins when you wear a certain color. If you wanted to see whether these are just coincidences or scientific fact, you would form a hypothesis, then create an experiment to see whether that hypothesis is true or not.

But what is a hypothesis, anyway? If you’re not sure about what a hypothesis is--or how to test for one!--you’re in the right place. This article will teach you everything you need to know about hypotheses, including: 

• Defining the term “hypothesis” 
• Providing hypothesis examples 
• Giving you tips for how to write your own hypothesis

So let’s get started!

What Is a Hypothesis?

Merriam Webster defines a hypothesis as “an assumption or concession made for the sake of argument.” In other words, a hypothesis is an educated guess . Scientists make a reasonable assumption--or a hypothesis--then design an experiment to test whether it’s true or not. Keep in mind that in science, a hypothesis should be testable. You have to be able to design an experiment that tests your hypothesis in order for it to be valid. 

As you could assume from that statement, it’s easy to make a bad hypothesis. But when you’re holding an experiment, it’s even more important that your guesses be good...after all, you’re spending time (and maybe money!) to figure out more about your observation. That’s why we refer to a hypothesis as an educated guess--good hypotheses are based on existing data and research to make them as sound as possible.

Hypotheses are one part of what’s called the scientific method .  Every (good) experiment or study is based in the scientific method. The scientific method gives order and structure to experiments and ensures that interference from scientists or outside influences does not skew the results. It’s important that you understand the concepts of the scientific method before holding your own experiment. Though it may vary among scientists, the scientific method is generally made up of six steps (in order):

• Observation
• Asking questions
• Forming a hypothesis
• Analyze the data
• Communicate your results

You’ll notice that the hypothesis comes pretty early on when conducting an experiment. That’s because experiments work best when they’re trying to answer one specific question. And you can’t conduct an experiment until you know what you’re trying to prove!

Independent and Dependent Variables 

After doing your research, you’re ready for another important step in forming your hypothesis: identifying variables. Variables are basically any factor that could influence the outcome of your experiment . Variables have to be measurable and related to the topic being studied.

There are two types of variables:  independent variables and dependent variables. I ndependent variables remain constant . For example, age is an independent variable; it will stay the same, and researchers can look at different ages to see if it has an effect on the dependent variable. 

Speaking of dependent variables... dependent variables are subject to the influence of the independent variable , meaning that they are not constant. Let’s say you want to test whether a person’s age affects how much sleep they need. In that case, the independent variable is age (like we mentioned above), and the dependent variable is how much sleep a person gets. 

Variables will be crucial in writing your hypothesis. You need to be able to identify which variable is which, as both the independent and dependent variables will be written into your hypothesis. For instance, in a study about exercise, the independent variable might be the speed at which the respondents walk for thirty minutes, and the dependent variable would be their heart rate. In your study and in your hypothesis, you’re trying to understand the relationship between the two variables.

Elements of a Good Hypothesis

The best hypotheses start by asking the right questions . For instance, if you’ve observed that the grass is greener when it rains twice a week, you could ask what kind of grass it is, what elevation it’s at, and if the grass across the street responds to rain in the same way. Any of these questions could become the backbone of experiments to test why the grass gets greener when it rains fairly frequently.

As you’re asking more questions about your first observation, make sure you’re also making more observations . If it doesn’t rain for two weeks and the grass still looks green, that’s an important observation that could influence your hypothesis. You'll continue observing all throughout your experiment, but until the hypothesis is finalized, every observation should be noted.

Finally, you should consult secondary research before writing your hypothesis . Secondary research is comprised of results found and published by other people. You can usually find this information online or at your library. Additionally, m ake sure the research you find is credible and related to your topic. If you’re studying the correlation between rain and grass growth, it would help you to research rain patterns over the past twenty years for your county, published by a local agricultural association. You should also research the types of grass common in your area, the type of grass in your lawn, and whether anyone else has conducted experiments about your hypothesis. Also be sure you’re checking the quality of your research . Research done by a middle school student about what minerals can be found in rainwater would be less useful than an article published by a local university.

Writing Your Hypothesis

Once you’ve considered all of the factors above, you’re ready to start writing your hypothesis. Hypotheses usually take a certain form when they’re written out in a research report.

When you boil down your hypothesis statement, you are writing down your best guess and not the question at hand . This means that your statement should be written as if it is fact already, even though you are simply testing it.

The reason for this is that, after you have completed your study, you'll either accept or reject your if-then or your null hypothesis. All hypothesis testing examples should be measurable and able to be confirmed or denied. You cannot confirm a question, only a statement! 

In fact, you come up with hypothesis examples all the time! For instance, when you guess on the outcome of a basketball game, you don’t say, “Will the Miami Heat beat the Boston Celtics?” but instead, “I think the Miami Heat will beat the Boston Celtics.” You state it as if it is already true, even if it turns out you’re wrong. You do the same thing when writing your hypothesis.

Additionally, keep in mind that hypotheses can range from very specific to very broad.  These hypotheses can be specific, but if your hypothesis testing examples involve a broad range of causes and effects, your hypothesis can also be broad.  

The Two Types of Hypotheses

Now that you understand what goes into a hypothesis, it’s time to look more closely at the two most common types of hypothesis: the if-then hypothesis and the null hypothesis.

#1: If-Then Hypotheses

First of all, if-then hypotheses typically follow this formula:

If ____ happens, then ____ will happen.

The goal of this type of hypothesis is to test the causal relationship between the independent and dependent variable. It’s fairly simple, and each hypothesis can vary in how detailed it can be. We create if-then hypotheses all the time with our daily predictions. Here are some examples of hypotheses that use an if-then structure from daily life: 

• If I get enough sleep, I’ll be able to get more work done tomorrow.
• If the bus is on time, I can make it to my friend’s birthday party. 
• If I study every night this week, I’ll get a better grade on my exam. 

In each of these situations, you’re making a guess on how an independent variable (sleep, time, or studying) will affect a dependent variable (the amount of work you can do, making it to a party on time, or getting better grades). 

You may still be asking, “What is an example of a hypothesis used in scientific research?” Take one of the hypothesis examples from a real-world study on whether using technology before bed affects children’s sleep patterns. The hypothesis read s:

“We hypothesized that increased hours of tablet- and phone-based screen time at bedtime would be inversely correlated with sleep quality and child attention.”

It might not look like it, but this is an if-then statement. The researchers basically said, “If children have more screen usage at bedtime, then their quality of sleep and attention will be worse.” The sleep quality and attention are the dependent variables and the screen usage is the independent variable. (Usually, the independent variable comes after the “if” and the dependent variable comes after the “then,” as it is the independent variable that affects the dependent variable.) This is an excellent example of how flexible hypothesis statements can be, as long as the general idea of “if-then” and the independent and dependent variables are present.

#2: Null Hypotheses

Your if-then hypothesis is not the only one needed to complete a successful experiment, however. You also need a null hypothesis to test it against. In its most basic form, the null hypothesis is the opposite of your if-then hypothesis . When you write your null hypothesis, you are writing a hypothesis that suggests that your guess is not true, and that the independent and dependent variables have no relationship .

One null hypothesis for the cell phone and sleep study from the last section might say: 

“If children have more screen usage at bedtime, their quality of sleep and attention will not be worse.” 

In this case, this is a null hypothesis because it’s asking the opposite of the original thesis! 

Conversely, if your if-then hypothesis suggests that your two variables have no relationship, then your null hypothesis would suggest that there is one. So, pretend that there is a study that is asking the question, “Does the amount of followers on Instagram influence how long people spend on the app?” The independent variable is the amount of followers, and the dependent variable is the time spent. But if you, as the researcher, don’t think there is a relationship between the number of followers and time spent, you might write an if-then hypothesis that reads:

“If people have many followers on Instagram, they will not spend more time on the app than people who have less.”

In this case, the if-then suggests there isn’t a relationship between the variables. In that case, one of the null hypothesis examples might say:

“If people have many followers on Instagram, they will spend more time on the app than people who have less.”

You then test both the if-then and the null hypothesis to gauge if there is a relationship between the variables, and if so, how much of a relationship. 

4 Tips to Write the Best Hypothesis

If you’re going to take the time to hold an experiment, whether in school or by yourself, you’re also going to want to take the time to make sure your hypothesis is a good one. The best hypotheses have four major elements in common: plausibility, defined concepts, observability, and general explanation.

#1: Plausibility

At first glance, this quality of a hypothesis might seem obvious. When your hypothesis is plausible, that means it’s possible given what we know about science and general common sense. However, improbable hypotheses are more common than you might think. 

Imagine you’re studying weight gain and television watching habits. If you hypothesize that people who watch more than  twenty hours of television a week will gain two hundred pounds or more over the course of a year, this might be improbable (though it’s potentially possible). Consequently, c ommon sense can tell us the results of the study before the study even begins.

Improbable hypotheses generally go against  science, as well. Take this hypothesis example: 

“If a person smokes one cigarette a day, then they will have lungs just as healthy as the average person’s.” 

This hypothesis is obviously untrue, as studies have shown again and again that cigarettes negatively affect lung health. You must be careful that your hypotheses do not reflect your own personal opinion more than they do scientifically-supported findings. This plausibility points to the necessity of research before the hypothesis is written to make sure that your hypothesis has not already been disproven.

#2: Defined Concepts

The more advanced you are in your studies, the more likely that the terms you’re using in your hypothesis are specific to a limited set of knowledge. One of the hypothesis testing examples might include the readability of printed text in newspapers, where you might use words like “kerning” and “x-height.” Unless your readers have a background in graphic design, it’s likely that they won’t know what you mean by these terms. Thus, it’s important to either write what they mean in the hypothesis itself or in the report before the hypothesis.

Here’s what we mean. Which of the following sentences makes more sense to the common person?

If the kerning is greater than average, more words will be read per minute.

If the space between letters is greater than average, more words will be read per minute.

For people reading your report that are not experts in typography, simply adding a few more words will be helpful in clarifying exactly what the experiment is all about. It’s always a good idea to make your research and findings as accessible as possible. 

Good hypotheses ensure that you can observe the results. 

#3: Observability

In order to measure the truth or falsity of your hypothesis, you must be able to see your variables and the way they interact. For instance, if your hypothesis is that the flight patterns of satellites affect the strength of certain television signals, yet you don’t have a telescope to view the satellites or a television to monitor the signal strength, you cannot properly observe your hypothesis and thus cannot continue your study.

Some variables may seem easy to observe, but if you do not have a system of measurement in place, you cannot observe your hypothesis properly. Here’s an example: if you’re experimenting on the effect of healthy food on overall happiness, but you don’t have a way to monitor and measure what “overall happiness” means, your results will not reflect the truth. Monitoring how often someone smiles for a whole day is not reasonably observable, but having the participants state how happy they feel on a scale of one to ten is more observable. 

In writing your hypothesis, always keep in mind how you'll execute the experiment.

#4: Generalizability 

Perhaps you’d like to study what color your best friend wears the most often by observing and documenting the colors she wears each day of the week. This might be fun information for her and you to know, but beyond you two, there aren’t many people who could benefit from this experiment. When you start an experiment, you should note how generalizable your findings may be if they are confirmed. Generalizability is basically how common a particular phenomenon is to other people’s everyday life.

Let’s say you’re asking a question about the health benefits of eating an apple for one day only, you need to realize that the experiment may be too specific to be helpful. It does not help to explain a phenomenon that many people experience. If you find yourself with too specific of a hypothesis, go back to asking the big question: what is it that you want to know, and what do you think will happen between your two variables?

Hypothesis Testing Examples

We know it can be hard to write a good hypothesis unless you’ve seen some good hypothesis examples. We’ve included four hypothesis examples based on some made-up experiments. Use these as templates or launch pads for coming up with your own hypotheses.

Experiment #1: Students Studying Outside (Writing a Hypothesis)

You are a student at PrepScholar University. When you walk around campus, you notice that, when the temperature is above 60 degrees, more students study in the quad. You want to know when your fellow students are more likely to study outside. With this information, how do you make the best hypothesis possible?

You must remember to make additional observations and do secondary research before writing your hypothesis. In doing so, you notice that no one studies outside when it’s 75 degrees and raining, so this should be included in your experiment. Also, studies done on the topic beforehand suggested that students are more likely to study in temperatures less than 85 degrees. With this in mind, you feel confident that you can identify your variables and write your hypotheses:

If-then: “If the temperature in Fahrenheit is less than 60 degrees, significantly fewer students will study outside.”

Null: “If the temperature in Fahrenheit is less than 60 degrees, the same number of students will study outside as when it is more than 60 degrees.”

These hypotheses are plausible, as the temperatures are reasonably within the bounds of what is possible. The number of people in the quad is also easily observable. It is also not a phenomenon specific to only one person or at one time, but instead can explain a phenomenon for a broader group of people.

To complete this experiment, you pick the month of October to observe the quad. Every day (except on the days where it’s raining)from 3 to 4 PM, when most classes have released for the day, you observe how many people are on the quad. You measure how many people come  and how many leave. You also write down the temperature on the hour. 

After writing down all of your observations and putting them on a graph, you find that the most students study on the quad when it is 70 degrees outside, and that the number of students drops a lot once the temperature reaches 60 degrees or below. In this case, your research report would state that you accept or “failed to reject” your first hypothesis with your findings.

Experiment #2: The Cupcake Store (Forming a Simple Experiment)

Let’s say that you work at a bakery. You specialize in cupcakes, and you make only two colors of frosting: yellow and purple. You want to know what kind of customers are more likely to buy what kind of cupcake, so you set up an experiment. Your independent variable is the customer’s gender, and the dependent variable is the color of the frosting. What is an example of a hypothesis that might answer the question of this study?

Here’s what your hypotheses might look like: 

If-then: “If customers’ gender is female, then they will buy more yellow cupcakes than purple cupcakes.”

Null: “If customers’ gender is female, then they will be just as likely to buy purple cupcakes as yellow cupcakes.”

This is a pretty simple experiment! It passes the test of plausibility (there could easily be a difference), defined concepts (there’s nothing complicated about cupcakes!), observability (both color and gender can be easily observed), and general explanation ( this would potentially help you make better business decisions ).

Experiment #3: Backyard Bird Feeders (Integrating Multiple Variables and Rejecting the If-Then Hypothesis)

While watching your backyard bird feeder, you realized that different birds come on the days when you change the types of seeds. You decide that you want to see more cardinals in your backyard, so you decide to see what type of food they like the best and set up an experiment. 

However, one morning, you notice that, while some cardinals are present, blue jays are eating out of your backyard feeder filled with millet. You decide that, of all of the other birds, you would like to see the blue jays the least. This means you'll have more than one variable in your hypothesis. Your new hypotheses might look like this: 

If-then: “If sunflower seeds are placed in the bird feeders, then more cardinals will come than blue jays. If millet is placed in the bird feeders, then more blue jays will come than cardinals.”

Null: “If either sunflower seeds or millet are placed in the bird, equal numbers of cardinals and blue jays will come.”

Through simple observation, you actually find that cardinals come as often as blue jays when sunflower seeds or millet is in the bird feeder. In this case, you would reject your “if-then” hypothesis and “fail to reject” your null hypothesis . You cannot accept your first hypothesis, because it’s clearly not true. Instead you found that there was actually no relation between your different variables. Consequently, you would need to run more experiments with different variables to see if the new variables impact the results.

Experiment #4: In-Class Survey (Including an Alternative Hypothesis)

You’re about to give a speech in one of your classes about the importance of paying attention. You want to take this opportunity to test a hypothesis you’ve had for a while: 

If-then: If students sit in the first two rows of the classroom, then they will listen better than students who do not.

Null: If students sit in the first two rows of the classroom, then they will not listen better or worse than students who do not.

You give your speech and then ask your teacher if you can hand out a short survey to the class. On the survey, you’ve included questions about some of the topics you talked about. When you get back the results, you’re surprised to see that not only do the students in the first two rows not pay better attention, but they also scored worse than students in other parts of the classroom! Here, both your if-then and your null hypotheses are not representative of your findings. What do you do?

This is when you reject both your if-then and null hypotheses and instead create an alternative hypothesis . This type of hypothesis is used in the rare circumstance that neither of your hypotheses is able to capture your findings . Now you can use what you’ve learned to draft new hypotheses and test again! 

Key Takeaways: Hypothesis Writing

The more comfortable you become with writing hypotheses, the better they will become. The structure of hypotheses is flexible and may need to be changed depending on what topic you are studying. The most important thing to remember is the purpose of your hypothesis and the difference between the if-then and the null . From there, in forming your hypothesis, you should constantly be asking questions, making observations, doing secondary research, and considering your variables. After you have written your hypothesis, be sure to edit it so that it is plausible, clearly defined, observable, and helpful in explaining a general phenomenon.

Writing a hypothesis is something that everyone, from elementary school children competing in a science fair to professional scientists in a lab, needs to know how to do. Hypotheses are vital in experiments and in properly executing the scientific method . When done correctly, hypotheses will set up your studies for success and help you to understand the world a little better, one experiment at a time.

What’s Next?

If you’re studying for the science portion of the ACT, there’s definitely a lot you need to know. We’ve got the tools to help, though! Start by checking out our ultimate study guide for the ACT Science subject test. Once you read through that, be sure to download our recommended ACT Science practice tests , since they’re one of the most foolproof ways to improve your score. (And don’t forget to check out our expert guide book , too.)

If you love science and want to major in a scientific field, you should start preparing in high school . Here are the science classes you should take to set yourself up for success.

If you’re trying to think of science experiments you can do for class (or for a science fair!), here’s a list of 37 awesome science experiments you can do at home

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Ashley Sufflé Robinson has a Ph.D. in 19th Century English Literature. As a content writer for PrepScholar, Ashley is passionate about giving college-bound students the in-depth information they need to get into the school of their dreams.

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Theories, Hypotheses, and Laws: Definitions, examples, and their roles in science

by Anthony Carpi, Ph.D., Anne E. Egger, Ph.D.

Listen to this reading

Did you know that the idea of evolution had been part of Western thought for more than 2,000 years before Charles Darwin was born? Like many theories, the theory of evolution was the result of the work of many different scientists working in different disciplines over a period of time.

A scientific theory is an explanation inferred from multiple lines of evidence for some broad aspect of the natural world and is logical, testable, and predictive.

As new evidence comes to light, or new interpretations of existing data are proposed, theories may be revised and even change; however, they are not tenuous or speculative.

A scientific hypothesis is an inferred explanation of an observation or research finding; while more exploratory in nature than a theory, it is based on existing scientific knowledge.

A scientific law is an expression of a mathematical or descriptive relationship observed in nature.

Imagine yourself shopping in a grocery store with a good friend who happens to be a chemist. Struggling to choose between the many different types of tomatoes in front of you, you pick one up, turn to your friend, and ask her if she thinks the tomato is organic . Your friend simply chuckles and replies, "Of course it's organic!" without even looking at how the fruit was grown. Why the amused reaction? Your friend is highlighting a simple difference in vocabulary. To a chemist, the term organic refers to any compound in which hydrogen is bonded to carbon. Tomatoes (like all plants) are abundant in organic compounds – thus your friend's laughter. In modern agriculture, however, organic has come to mean food items grown or raised without the use of chemical fertilizers, pesticides, or other additives.

So who is correct? You both are. Both uses of the word are correct, though they mean different things in different contexts. There are, of course, lots of words that have more than one meaning (like bat , for example), but multiple meanings can be especially confusing when two meanings convey very different ideas and are specific to one field of study.

• Scientific theories

The term theory also has two meanings, and this double meaning often leads to confusion. In common language, the term theory generally refers to speculation or a hunch or guess. You might have a theory about why your favorite sports team isn't playing well, or who ate the last cookie from the cookie jar. But these theories do not fit the scientific use of the term. In science, a theory is a well-substantiated and comprehensive set of ideas that explains a phenomenon in nature. A scientific theory is based on large amounts of data and observations that have been collected over time. Scientific theories can be tested and refined by additional research , and they allow scientists to make predictions. Though you may be correct in your hunch, your cookie jar conjecture doesn't fit this more rigorous definition.

All scientific disciplines have well-established, fundamental theories . For example, atomic theory describes the nature of matter and is supported by multiple lines of evidence from the way substances behave and react in the world around us (see our series on Atomic Theory ). Plate tectonic theory describes the large scale movement of the outer layer of the Earth and is supported by evidence from studies about earthquakes , magnetic properties of the rocks that make up the seafloor , and the distribution of volcanoes on Earth (see our series on Plate Tectonic Theory ). The theory of evolution by natural selection , which describes the mechanism by which inherited traits that affect survivability or reproductive success can cause changes in living organisms over generations , is supported by extensive studies of DNA , fossils , and other types of scientific evidence (see our Charles Darwin series for more information). Each of these major theories guides and informs modern research in those fields, integrating a broad, comprehensive set of ideas.

So how are these fundamental theories developed, and why are they considered so well supported? Let's take a closer look at some of the data and research supporting the theory of natural selection to better see how a theory develops.

Comprehension Checkpoint

• The development of a scientific theory: Evolution and natural selection

The theory of evolution by natural selection is sometimes maligned as Charles Darwin 's speculation on the origin of modern life forms. However, evolutionary theory is not speculation. While Darwin is rightly credited with first articulating the theory of natural selection, his ideas built on more than a century of scientific research that came before him, and are supported by over a century and a half of research since.

• The Fixity Notion: Linnaeus

Figure 1: Cover of the 1760 edition of Systema Naturae .

Research about the origins and diversity of life proliferated in the 18th and 19th centuries. Carolus Linnaeus , a Swedish botanist and the father of modern taxonomy (see our module Taxonomy I for more information), was a devout Christian who believed in the concept of Fixity of Species , an idea based on the biblical story of creation. The Fixity of Species concept said that each species is based on an ideal form that has not changed over time. In the early stages of his career, Linnaeus traveled extensively and collected data on the structural similarities and differences between different species of plants. Noting that some very different plants had similar structures, he began to piece together his landmark work, Systema Naturae, in 1735 (Figure 1). In Systema , Linnaeus classified organisms into related groups based on similarities in their physical features. He developed a hierarchical classification system , even drawing relationships between seemingly disparate species (for example, humans, orangutans, and chimpanzees) based on the physical similarities that he observed between these organisms. Linnaeus did not explicitly discuss change in organisms or propose a reason for his hierarchy, but by grouping organisms based on physical characteristics, he suggested that species are related, unintentionally challenging the Fixity notion that each species is created in a unique, ideal form.

• The age of Earth: Leclerc and Hutton

Also in the early 1700s, Georges-Louis Leclerc, a French naturalist, and James Hutton , a Scottish geologist, began to develop new ideas about the age of the Earth. At the time, many people thought of the Earth as 6,000 years old, based on a strict interpretation of the events detailed in the Christian Old Testament by the influential Scottish Archbishop Ussher. By observing other planets and comets in the solar system , Leclerc hypothesized that Earth began as a hot, fiery ball of molten rock, mostly consisting of iron. Using the cooling rate of iron, Leclerc calculated that Earth must therefore be at least 70,000 years old in order to have reached its present temperature.

Hutton approached the same topic from a different perspective, gathering observations of the relationships between different rock formations and the rates of modern geological processes near his home in Scotland. He recognized that the relatively slow processes of erosion and sedimentation could not create all of the exposed rock layers in only a few thousand years (see our module The Rock Cycle ). Based on his extensive collection of data (just one of his many publications ran to 2,138 pages), Hutton suggested that the Earth was far older than human history – hundreds of millions of years old.

While we now know that both Leclerc and Hutton significantly underestimated the age of the Earth (by about 4 billion years), their work shattered long-held beliefs and opened a window into research on how life can change over these very long timescales.

• Fossil studies lead to the development of a theory of evolution: Cuvier

Figure 2: Illustration of an Indian elephant jaw and a mammoth jaw from Cuvier's 1796 paper.

With the age of Earth now extended by Leclerc and Hutton, more researchers began to turn their attention to studying past life. Fossils are the main way to study past life forms, and several key studies on fossils helped in the development of a theory of evolution . In 1795, Georges Cuvier began to work at the National Museum in Paris as a naturalist and anatomist. Through his work, Cuvier became interested in fossils found near Paris, which some claimed were the remains of the elephants that Hannibal rode over the Alps when he invaded Rome in 218 BCE . In studying both the fossils and living species , Cuvier documented different patterns in the dental structure and number of teeth between the fossils and modern elephants (Figure 2) (Horner, 1843). Based on these data , Cuvier hypothesized that the fossil remains were not left by Hannibal, but were from a distinct species of animal that once roamed through Europe and had gone extinct thousands of years earlier: the mammoth. The concept of species extinction had been discussed by a few individuals before Cuvier, but it was in direct opposition to the Fixity of Species concept – if every organism were based on a perfectly adapted, ideal form, how could any cease to exist? That would suggest it was no longer ideal.

While his work provided critical evidence of extinction , a key component of evolution , Cuvier was highly critical of the idea that species could change over time. As a result of his extensive studies of animal anatomy, Cuvier had developed a holistic view of organisms , stating that the

number, direction, and shape of the bones that compose each part of an animal's body are always in a necessary relation to all the other parts, in such a way that ... one can infer the whole from any one of them ...

In other words, Cuvier viewed each part of an organism as a unique, essential component of the whole organism. If one part were to change, he believed, the organism could not survive. His skepticism about the ability of organisms to change led him to criticize the whole idea of evolution , and his prominence in France as a scientist played a large role in discouraging the acceptance of the idea in the scientific community.

• Studies of invertebrates support a theory of change in species: Lamarck

Jean Baptiste Lamarck, a contemporary of Cuvier's at the National Museum in Paris, studied invertebrates like insects and worms. As Lamarck worked through the museum's large collection of invertebrates, he was impressed by the number and variety of organisms . He became convinced that organisms could, in fact, change through time, stating that

... time and favorable conditions are the two principal means which nature has employed in giving existence to all her productions. We know that for her time has no limit, and that consequently she always has it at her disposal.

This was a radical departure from both the fixity concept and Cuvier's ideas, and it built on the long timescale that geologists had recently established. Lamarck proposed that changes that occurred during an organism 's lifetime could be passed on to their offspring, suggesting, for example, that a body builder's muscles would be inherited by their children.

As it turned out, the mechanism by which Lamarck proposed that organisms change over time was wrong, and he is now often referred to disparagingly for his "inheritance of acquired characteristics" idea. Yet despite the fact that some of his ideas were discredited, Lamarck established a support for evolutionary theory that others would build on and improve.

• Rock layers as evidence for evolution: Smith

In the early 1800s, a British geologist and canal surveyor named William Smith added another component to the accumulating evidence for evolution . Smith observed that rock layers exposed in different parts of England bore similarities to one another: These layers (or strata) were arranged in a predictable order, and each layer contained distinct groups of fossils . From this series of observations , he developed a hypothesis that specific groups of animals followed one another in a definite sequence through Earth's history, and this sequence could be seen in the rock layers. Smith's hypothesis was based on his knowledge of geological principles , including the Law of Superposition.

The Law of Superposition states that sediments are deposited in a time sequence, with the oldest sediments deposited first, or at the bottom, and newer layers deposited on top. The concept was first expressed by the Persian scientist Avicenna in the 11th century, but was popularized by the Danish scientist Nicolas Steno in the 17th century. Note that the law does not state how sediments are deposited; it simply describes the relationship between the ages of deposited sediments.

Figure 3: Engraving from William Smith's 1815 monograph on identifying strata by fossils.

Smith backed up his hypothesis with extensive drawings of fossils uncovered during his research (Figure 3), thus allowing other scientists to confirm or dispute his findings. His hypothesis has, in fact, been confirmed by many other scientists and has come to be referred to as the Law of Faunal Succession. His work was critical to the formation of evolutionary theory as it not only confirmed Cuvier's work that organisms have gone extinct , but it also showed that the appearance of life does not date to the birth of the planet. Instead, the fossil record preserves a timeline of the appearance and disappearance of different organisms in the past, and in doing so offers evidence for change in organisms over time.

• The theory of evolution by natural selection: Darwin and Wallace

It was into this world that Charles Darwin entered: Linnaeus had developed a taxonomy of organisms based on their physical relationships, Leclerc and Hutton demonstrated that there was sufficient time in Earth's history for organisms to change, Cuvier showed that species of organisms have gone extinct , Lamarck proposed that organisms change over time, and Smith established a timeline of the appearance and disappearance of different organisms in the geological record .

Figure 4: Title page of the 1859 Murray edition of the Origin of Species by Charles Darwin.

Charles Darwin collected data during his work as a naturalist on the HMS Beagle starting in 1831. He took extensive notes on the geology of the places he visited; he made a major find of fossils of extinct animals in Patagonia and identified an extinct giant ground sloth named Megatherium . He experienced an earthquake in Chile that stranded beds of living mussels above water, where they would be preserved for years to come.

Perhaps most famously, he conducted extensive studies of animals on the Galápagos Islands, noting subtle differences in species of mockingbird, tortoise, and finch that were isolated on different islands with different environmental conditions. These subtle differences made the animals highly adapted to their environments .

This broad spectrum of data led Darwin to propose an idea about how organisms change "by means of natural selection" (Figure 4). But this idea was not based only on his work, it was also based on the accumulation of evidence and ideas of many others before him. Because his proposal encompassed and explained many different lines of evidence and previous work, they formed the basis of a new and robust scientific theory regarding change in organisms – the theory of evolution by natural selection .

Darwin's ideas were grounded in evidence and data so compelling that if he had not conceived them, someone else would have. In fact, someone else did. Between 1858 and 1859, Alfred Russel Wallace , a British naturalist, wrote a series of letters to Darwin that independently proposed natural selection as the means for evolutionary change. The letters were presented to the Linnean Society of London, a prominent scientific society at the time (see our module on Scientific Institutions and Societies ). This long chain of research highlights that theories are not just the work of one individual. At the same time, however, it often takes the insight and creativity of individuals to put together all of the pieces and propose a new theory . Both Darwin and Wallace were experienced naturalists who were familiar with the work of others. While all of the work leading up to 1830 contributed to the theory of evolution , Darwin's and Wallace's theory changed the way that future research was focused by presenting a comprehensive, well-substantiated set of ideas, thus becoming a fundamental theory of biological research.

• Expanding, testing, and refining scientific theories
• Genetics and evolution: Mendel and Dobzhansky

Since Darwin and Wallace first published their ideas, extensive research has tested and expanded the theory of evolution by natural selection . Darwin had no concept of genes or DNA or the mechanism by which characteristics were inherited within a species . A contemporary of Darwin's, the Austrian monk Gregor Mendel , first presented his own landmark study, Experiments in Plant Hybridization, in 1865 in which he provided the basic patterns of genetic inheritance , describing which characteristics (and evolutionary changes) can be passed on in organisms (see our Genetics I module for more information). Still, it wasn't until much later that a "gene" was defined as the heritable unit.

In 1937, the Ukrainian born geneticist Theodosius Dobzhansky published Genetics and the Origin of Species , a seminal work in which he described genes themselves and demonstrated that it is through mutations in genes that change occurs. The work defined evolution as "a change in the frequency of an allele within a gene pool" ( Dobzhansky, 1982 ). These studies and others in the field of genetics have added to Darwin's work, expanding the scope of the theory .

• Evolution under a microscope: Lenski

More recently, Dr. Richard Lenski, a scientist at Michigan State University, isolated a single Escherichia coli bacterium in 1989 as the first step of the longest running experimental test of evolutionary theory to date – a true test meant to replicate evolution and natural selection in the lab.

After the single microbe had multiplied, Lenski isolated the offspring into 12 different strains , each in their own glucose-supplied culture, predicting that the genetic make-up of each strain would change over time to become more adapted to their specific culture as predicted by evolutionary theory . These 12 lines have been nurtured for over 40,000 bacterial generations (luckily bacterial generations are much shorter than human generations) and exposed to different selective pressures such as heat , cold, antibiotics, and infection with other microorganisms. Lenski and colleagues have studied dozens of aspects of evolutionary theory with these genetically isolated populations . In 1999, they published a paper that demonstrated that random genetic mutations were common within the populations and highly diverse across different individual bacteria . However, "pivotal" mutations that are associated with beneficial changes in the group are shared by all descendants in a population and are much rarer than random mutations, as predicted by the theory of evolution by natural selection (Papadopoulos et al., 1999).

• Punctuated equilibrium: Gould and Eldredge

While established scientific theories like evolution have a wealth of research and evidence supporting them, this does not mean that they cannot be refined as new information or new perspectives on existing data become available. For example, in 1972, biologist Stephen Jay Gould and paleontologist Niles Eldredge took a fresh look at the existing data regarding the timing by which evolutionary change takes place. Gould and Eldredge did not set out to challenge the theory of evolution; rather they used it as a guiding principle and asked more specific questions to add detail and nuance to the theory. This is true of all theories in science: they provide a framework for additional research. At the time, many biologists viewed evolution as occurring gradually, causing small incremental changes in organisms at a relatively steady rate. The idea is referred to as phyletic gradualism , and is rooted in the geological concept of uniformitarianism . After reexamining the available data, Gould and Eldredge came to a different explanation, suggesting that evolution consists of long periods of stability that are punctuated by occasional instances of dramatic change – a process they called punctuated equilibrium .

Like Darwin before them, their proposal is rooted in evidence and research on evolutionary change, and has been supported by multiple lines of evidence. In fact, punctuated equilibrium is now considered its own theory in evolutionary biology. Punctuated equilibrium is not as broad of a theory as natural selection . In science, some theories are broad and overarching of many concepts, such as the theory of evolution by natural selection; others focus on concepts at a smaller, or more targeted, scale such as punctuated equilibrium. And punctuated equilibrium does not challenge or weaken the concept of natural selection; rather, it represents a change in our understanding of the timing by which change occurs in organisms , and a theory within a theory. The theory of evolution by natural selection now includes both gradualism and punctuated equilibrium to describe the rate at which change proceeds.

• Hypotheses and laws: Other scientific concepts

One of the challenges in understanding scientific terms like theory is that there is not a precise definition even within the scientific community. Some scientists debate over whether certain proposals merit designation as a hypothesis or theory , and others mistakenly use the terms interchangeably. But there are differences in these terms. A hypothesis is a proposed explanation for an observable phenomenon. Hypotheses , just like theories , are based on observations from research . For example, LeClerc did not hypothesize that Earth had cooled from a molten ball of iron as a random guess; rather, he developed this hypothesis based on his observations of information from meteorites.

A scientist often proposes a hypothesis before research confirms it as a way of predicting the outcome of study to help better define the parameters of the research. LeClerc's hypothesis allowed him to use known parameters (the cooling rate of iron) to do additional work. A key component of a formal scientific hypothesis is that it is testable and falsifiable. For example, when Richard Lenski first isolated his 12 strains of bacteria , he likely hypothesized that random mutations would cause differences to appear within a period of time in the different strains of bacteria. But when a hypothesis is generated in science, a scientist will also make an alternative hypothesis , an explanation that explains a study if the data do not support the original hypothesis. If the different strains of bacteria in Lenski's work did not diverge over the indicated period of time, perhaps the rate of mutation was slower than first thought.

So you might ask, if theories are so well supported, do they eventually become laws? The answer is no – not because they aren't well-supported, but because theories and laws are two very different things. Laws describe phenomena, often mathematically. Theories, however, explain phenomena. For example, in 1687 Isaac Newton proposed a Theory of Gravitation, describing gravity as a force of attraction between two objects. As part of this theory, Newton developed a Law of Universal Gravitation that explains how this force operates. This law states that the force of gravity between two objects is inversely proportional to the square of the distance between those objects. Newton 's Law does not explain why this is true, but it describes how gravity functions (see our Gravity: Newtonian Relationships module for more detail). In 1916, Albert Einstein developed his theory of general relativity to explain the mechanism by which gravity has its effect. Einstein's work challenges Newton's theory, and has been found after extensive testing and research to more accurately describe the phenomenon of gravity. While Einstein's work has replaced Newton's as the dominant explanation of gravity in modern science, Newton's Law of Universal Gravitation is still used as it reasonably (and more simply) describes the force of gravity under many conditions. Similarly, the Law of Faunal Succession developed by William Smith does not explain why organisms follow each other in distinct, predictable ways in the rock layers, but it accurately describes the phenomenon.

When your dog dies, does your cat grieve? Science says yes

Study of felines finds they sleep less, play less, meow more and seek attention when another cat (or even a dog) in the house passes away

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Losing a pet is like losing a member of your family. When it happens, everyone in the family feels it. But what about other pets?

In the cheekily titled paper “Is companion animal loss cat-astrophic? Responses of domestic cats to the loss of another companion animal,” scientists Brittany Greene and Jennifer Vonk of Oakland University in Michigan suggest that not only do cats feel the loss of another pet in the household, they demonstrate those feelings. The paper appears in the latest issue of Applied Animal Behaviour Science .

To test the hypothesis, the researchers recruited 412 cat caregivers (and 452 cats) to report on their feelings and the perceived feelings of the felines following the death of another companion animal in the household.

While admitting the study is less scientific than one might prefer — they caution against “anthropomorphism in the projection of caregiver grief onto surviving companion animals” — the researchers were able to report that cat caregivers saw a decrease in their cats sleeping, eating and playing following the death of another pet.

They also noticed higher levels of attention-seeking behaviour, particularly among cats with a higher level of attachment to their owners, and also those who had lived longer with the deceased animal.

What’s more, the species of the deceased animal didn’t seem to matter.

“Cats did not respond significantly differently to the loss of a companion dog or another cat,” the researchers wrote, noting: “Despite different communication signals, dogs and cats can live peacefully together.”

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They added: “Many cats and dogs sharing a household were reported to sleep and play together. Therefore, cats could have also responded to losing a positive relationship with a dog as a possible interspecific ‘ preferred associate.'” (This sounds like the term a cat would use when referring to a dog or possibly even its owner.)

Numerous studies on dogs have shown similar responses to the death of another pet. However, the researchers write, “ This is only the second known exploration of domestic cats’ responses to the death of another companion animal and reveals that cats exhibit similar grief-like behavioural changes following such deaths compared to dogs examined in previous work.”

This is something of a surprise, given that cats have been domesticated much more recently, descend from a different evolutionary branch and express themselves differently than dogs. “ Whereas dogs, descended from pack animals, might reasonably respond more strongly to the death of a conspecific, cats under human care have adapted to live among conspecifics and their capacity to respond to the loss of a companion warrants further study,” they wrote.

But other studies — OK, that one other study — did also show changes in behaviour among dogs and cats alike, including attention seeking, increased time spent in the deceased companion animal’s “favourite spot,” seeming to look for the animal and, in the case of cats, louder and more frequent vocalizations following the loss.

The study had many potential pitfalls, as the researchers themselves pointed out. Caregivers might project their own grief onto their cats. They might seek comfort from the cat and thus be more likely to notice the cat’s behaviour. Or it could be that the caregivers ’ grief, rather than that of the cats themselves, altered the surviving cats ’ behaviours.

Even so, they write: “The current study adds to the very limited data on social cognition of cats. Researchers are only beginning to address the paucity of empirical data on nonhuman understanding of death —   particularly in cross-species interactions — and the current study adds to this growing area by showing that cats may be impacted by the death of companions and more strongly so when they have spent more time engaged in more activities and have more positive relationships with the lost companion.”

They add: “Our results are consistent with the idea that cats may experience the loss of companion animals in ways similar to what dogs experience despite having evolved from a less social ancestor, and may contribute to shifts in our conceptualization of cats as asocial and aloof.”

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