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Statistics By Jim

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Null Hypothesis: Definition, Rejecting & Examples

By Jim Frost 6 Comments

What is a Null Hypothesis?

The null hypothesis in statistics states that there is no difference between groups or no relationship between variables. It is one of two mutually exclusive hypotheses about a population in a hypothesis test.

Photograph of Rodin's statue, The Thinker who is pondering the null hypothesis.

  • Null Hypothesis H 0 : No effect exists in the population.
  • Alternative Hypothesis H A : The effect exists in the population.

In every study or experiment, researchers assess an effect or relationship. This effect can be the effectiveness of a new drug, building material, or other intervention that has benefits. There is a benefit or connection that the researchers hope to identify. Unfortunately, no effect may exist. In statistics, we call this lack of an effect the null hypothesis. Researchers assume that this notion of no effect is correct until they have enough evidence to suggest otherwise, similar to how a trial presumes innocence.

In this context, the analysts don’t necessarily believe the null hypothesis is correct. In fact, they typically want to reject it because that leads to more exciting finds about an effect or relationship. The new vaccine works!

You can think of it as the default theory that requires sufficiently strong evidence to reject. Like a prosecutor, researchers must collect sufficient evidence to overturn the presumption of no effect. Investigators must work hard to set up a study and a data collection system to obtain evidence that can reject the null hypothesis.

Related post : What is an Effect in Statistics?

Null Hypothesis Examples

Null hypotheses start as research questions that the investigator rephrases as a statement indicating there is no effect or relationship.

Does the vaccine prevent infections? The vaccine does not affect the infection rate.
Does the new additive increase product strength? The additive does not affect mean product strength.
Does the exercise intervention increase bone mineral density? The intervention does not affect bone mineral density.
As screen time increases, does test performance decrease? There is no relationship between screen time and test performance.

After reading these examples, you might think they’re a bit boring and pointless. However, the key is to remember that the null hypothesis defines the condition that the researchers need to discredit before suggesting an effect exists.

Let’s see how you reject the null hypothesis and get to those more exciting findings!

When to Reject the Null Hypothesis

So, you want to reject the null hypothesis, but how and when can you do that? To start, you’ll need to perform a statistical test on your data. The following is an overview of performing a study that uses a hypothesis test.

The first step is to devise a research question and the appropriate null hypothesis. After that, the investigators need to formulate an experimental design and data collection procedures that will allow them to gather data that can answer the research question. Then they collect the data. For more information about designing a scientific study that uses statistics, read my post 5 Steps for Conducting Studies with Statistics .

After data collection is complete, statistics and hypothesis testing enter the picture. Hypothesis testing takes your sample data and evaluates how consistent they are with the null hypothesis. The p-value is a crucial part of the statistical results because it quantifies how strongly the sample data contradict the null hypothesis.

When the sample data provide sufficient evidence, you can reject the null hypothesis. In a hypothesis test, this process involves comparing the p-value to your significance level .

Rejecting the Null Hypothesis

Reject the null hypothesis when the p-value is less than or equal to your significance level. Your sample data favor the alternative hypothesis, which suggests that the effect exists in the population. For a mnemonic device, remember—when the p-value is low, the null must go!

When you can reject the null hypothesis, your results are statistically significant. Learn more about Statistical Significance: Definition & Meaning .

Failing to Reject the Null Hypothesis

Conversely, when the p-value is greater than your significance level, you fail to reject the null hypothesis. The sample data provides insufficient data to conclude that the effect exists in the population. When the p-value is high, the null must fly!

Note that failing to reject the null is not the same as proving it. For more information about the difference, read my post about Failing to Reject the Null .

That’s a very general look at the process. But I hope you can see how the path to more exciting findings depends on being able to rule out the less exciting null hypothesis that states there’s nothing to see here!

Let’s move on to learning how to write the null hypothesis for different types of effects, relationships, and tests.

Related posts : How Hypothesis Tests Work and Interpreting P-values

How to Write a Null Hypothesis

The null hypothesis varies by the type of statistic and hypothesis test. Remember that inferential statistics use samples to draw conclusions about populations. Consequently, when you write a null hypothesis, it must make a claim about the relevant population parameter . Further, that claim usually indicates that the effect does not exist in the population. Below are typical examples of writing a null hypothesis for various parameters and hypothesis tests.

Related posts : Descriptive vs. Inferential Statistics and Populations, Parameters, and Samples in Inferential Statistics

Group Means

T-tests and ANOVA assess the differences between group means. For these tests, the null hypothesis states that there is no difference between group means in the population. In other words, the experimental conditions that define the groups do not affect the mean outcome. Mu (µ) is the population parameter for the mean, and you’ll need to include it in the statement for this type of study.

For example, an experiment compares the mean bone density changes for a new osteoporosis medication. The control group does not receive the medicine, while the treatment group does. The null states that the mean bone density changes for the control and treatment groups are equal.

  • Null Hypothesis H 0 : Group means are equal in the population: µ 1 = µ 2 , or µ 1 – µ 2 = 0
  • Alternative Hypothesis H A : Group means are not equal in the population: µ 1 ≠ µ 2 , or µ 1 – µ 2 ≠ 0.

Group Proportions

Proportions tests assess the differences between group proportions. For these tests, the null hypothesis states that there is no difference between group proportions. Again, the experimental conditions did not affect the proportion of events in the groups. P is the population proportion parameter that you’ll need to include.

For example, a vaccine experiment compares the infection rate in the treatment group to the control group. The treatment group receives the vaccine, while the control group does not. The null states that the infection rates for the control and treatment groups are equal.

  • Null Hypothesis H 0 : Group proportions are equal in the population: p 1 = p 2 .
  • Alternative Hypothesis H A : Group proportions are not equal in the population: p 1 ≠ p 2 .

Correlation and Regression Coefficients

Some studies assess the relationship between two continuous variables rather than differences between groups.

In these studies, analysts often use either correlation or regression analysis . For these tests, the null states that there is no relationship between the variables. Specifically, it says that the correlation or regression coefficient is zero. As one variable increases, there is no tendency for the other variable to increase or decrease. Rho (ρ) is the population correlation parameter and beta (β) is the regression coefficient parameter.

For example, a study assesses the relationship between screen time and test performance. The null states that there is no correlation between this pair of variables. As screen time increases, test performance does not tend to increase or decrease.

  • Null Hypothesis H 0 : The correlation in the population is zero: ρ = 0.
  • Alternative Hypothesis H A : The correlation in the population is not zero: ρ ≠ 0.

For all these cases, the analysts define the hypotheses before the study. After collecting the data, they perform a hypothesis test to determine whether they can reject the null hypothesis.

The preceding examples are all for two-tailed hypothesis tests. To learn about one-tailed tests and how to write a null hypothesis for them, read my post One-Tailed vs. Two-Tailed Tests .

Related post : Understanding Correlation

Neyman, J; Pearson, E. S. (January 1, 1933).  On the Problem of the most Efficient Tests of Statistical Hypotheses .  Philosophical Transactions of the Royal Society A .  231  (694–706): 289–337.

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January 11, 2024 at 2:57 pm

Thanks for the reply.

January 10, 2024 at 1:23 pm

Hi Jim, In your comment you state that equivalence test null and alternate hypotheses are reversed. For hypothesis tests of data fits to a probability distribution, the null hypothesis is that the probability distribution fits the data. Is this correct?

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January 10, 2024 at 2:15 pm

Those two separate things, equivalence testing and normality tests. But, yes, you’re correct for both.

Hypotheses are switched for equivalence testing. You need to “work” (i.e., collect a large sample of good quality data) to be able to reject the null that the groups are different to be able to conclude they’re the same.

With typical hypothesis tests, if you have low quality data and a low sample size, you’ll fail to reject the null that they’re the same, concluding they’re equivalent. But that’s more a statement about the low quality and small sample size than anything to do with the groups being equal.

So, equivalence testing make you work to obtain a finding that the groups are the same (at least within some amount you define as a trivial difference).

For normality testing, and other distribution tests, the null states that the data follow the distribution (normal or whatever). If you reject the null, you have sufficient evidence to conclude that your sample data don’t follow the probability distribution. That’s a rare case where you hope to fail to reject the null. And it suffers from the problem I describe above where you might fail to reject the null simply because you have a small sample size. In that case, you’d conclude the data follow the probability distribution but it’s more that you don’t have enough data for the test to register the deviation. In this scenario, if you had a larger sample size, you’d reject the null and conclude it doesn’t follow that distribution.

I don’t know of any equivalence testing type approach for distribution fit tests where you’d need to work to show the data follow a distribution, although I haven’t looked for one either!

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February 20, 2022 at 9:26 pm

Is a null hypothesis regularly (always) stated in the negative? “there is no” or “does not”

February 23, 2022 at 9:21 pm

Typically, the null hypothesis includes an equal sign. The null hypothesis states that the population parameter equals a particular value. That value is usually one that represents no effect. In the case of a one-sided hypothesis test, the null still contains an equal sign but it’s “greater than or equal to” or “less than or equal to.” If you wanted to translate the null hypothesis from its native mathematical expression, you could use the expression “there is no effect.” But the mathematical form more specifically states what it’s testing.

It’s the alternative hypothesis that typically contains does not equal.

There are some exceptions. For example, in an equivalence test where the researchers want to show that two things are equal, the null hypothesis states that they’re not equal.

In short, the null hypothesis states the condition that the researchers hope to reject. They need to work hard to set up an experiment and data collection that’ll gather enough evidence to be able to reject the null condition.

' src=

February 15, 2022 at 9:32 am

Dear sir I always read your notes on Research methods.. Kindly tell is there any available Book on all these..wonderfull Urgent

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  • Null and Alternative Hypotheses | Definitions & Examples

Null and Alternative Hypotheses | Definitions & Examples

Published on 5 October 2022 by Shaun Turney . Revised on 6 December 2022.

The null and alternative hypotheses are two competing claims that researchers weigh evidence for and against using a statistical test :

  • Null hypothesis (H 0 ): There’s no effect in the population .
  • Alternative hypothesis (H A ): There’s an effect in the population.

The effect is usually the effect of the independent variable on the dependent variable .

Table of contents

Answering your research question with hypotheses, what is a null hypothesis, what is an alternative hypothesis, differences between null and alternative hypotheses, how to write null and alternative hypotheses, frequently asked questions about null and alternative hypotheses.

The null and alternative hypotheses offer competing answers to your research question . When the research question asks “Does the independent variable affect the dependent variable?”, the null hypothesis (H 0 ) answers “No, there’s no effect in the population.” On the other hand, the alternative hypothesis (H A ) answers “Yes, there is an effect in the population.”

The null and alternative are always claims about the population. That’s because the goal of hypothesis testing is to make inferences about a population based on a sample . Often, we infer whether there’s an effect in the population by looking at differences between groups or relationships between variables in the sample.

You can use a statistical test to decide whether the evidence favors the null or alternative hypothesis. Each type of statistical test comes with a specific way of phrasing the null and alternative hypothesis. However, the hypotheses can also be phrased in a general way that applies to any test.

The null hypothesis is the claim that there’s no effect in the population.

If the sample provides enough evidence against the claim that there’s no effect in the population ( p ≤ α), then we can reject the null hypothesis . Otherwise, we fail to reject the null hypothesis.

Although “fail to reject” may sound awkward, it’s the only wording that statisticians accept. Be careful not to say you “prove” or “accept” the null hypothesis.

Null hypotheses often include phrases such as “no effect”, “no difference”, or “no relationship”. When written in mathematical terms, they always include an equality (usually =, but sometimes ≥ or ≤).

Examples of null hypotheses

The table below gives examples of research questions and null hypotheses. There’s always more than one way to answer a research question, but these null hypotheses can help you get started.

( )
Does tooth flossing affect the number of cavities? Tooth flossing has on the number of cavities. test:

The mean number of cavities per person does not differ between the flossing group (µ ) and the non-flossing group (µ ) in the population; µ = µ .

Does the amount of text highlighted in the textbook affect exam scores? The amount of text highlighted in the textbook has on exam scores. :

There is no relationship between the amount of text highlighted and exam scores in the population; β = 0.

Does daily meditation decrease the incidence of depression? Daily meditation the incidence of depression.* test:

The proportion of people with depression in the daily-meditation group ( ) is greater than or equal to the no-meditation group ( ) in the population; ≥ .

*Note that some researchers prefer to always write the null hypothesis in terms of “no effect” and “=”. It would be fine to say that daily meditation has no effect on the incidence of depression and p 1 = p 2 .

The alternative hypothesis (H A ) is the other answer to your research question . It claims that there’s an effect in the population.

Often, your alternative hypothesis is the same as your research hypothesis. In other words, it’s the claim that you expect or hope will be true.

The alternative hypothesis is the complement to the null hypothesis. Null and alternative hypotheses are exhaustive, meaning that together they cover every possible outcome. They are also mutually exclusive, meaning that only one can be true at a time.

Alternative hypotheses often include phrases such as “an effect”, “a difference”, or “a relationship”. When alternative hypotheses are written in mathematical terms, they always include an inequality (usually ≠, but sometimes > or <). As with null hypotheses, there are many acceptable ways to phrase an alternative hypothesis.

Examples of alternative hypotheses

The table below gives examples of research questions and alternative hypotheses to help you get started with formulating your own.

Does tooth flossing affect the number of cavities? Tooth flossing has an on the number of cavities. test:

The mean number of cavities per person differs between the flossing group (µ ) and the non-flossing group (µ ) in the population; µ ≠ µ .

Does the amount of text highlighted in a textbook affect exam scores? The amount of text highlighted in the textbook has an on exam scores. :

There is a relationship between the amount of text highlighted and exam scores in the population; β ≠ 0.

Does daily meditation decrease the incidence of depression? Daily meditation the incidence of depression. test:

The proportion of people with depression in the daily-meditation group ( ) is less than the no-meditation group ( ) in the population; < .

Null and alternative hypotheses are similar in some ways:

  • They’re both answers to the research question
  • They both make claims about the population
  • They’re both evaluated by statistical tests.

However, there are important differences between the two types of hypotheses, summarized in the following table.

A claim that there is in the population. A claim that there is in the population.

Equality symbol (=, ≥, or ≤) Inequality symbol (≠, <, or >)
Rejected Supported
Failed to reject Not supported

To help you write your hypotheses, you can use the template sentences below. If you know which statistical test you’re going to use, you can use the test-specific template sentences. Otherwise, you can use the general template sentences.

The only thing you need to know to use these general template sentences are your dependent and independent variables. To write your research question, null hypothesis, and alternative hypothesis, fill in the following sentences with your variables:

Does independent variable affect dependent variable ?

  • Null hypothesis (H 0 ): Independent variable does not affect dependent variable .
  • Alternative hypothesis (H A ): Independent variable affects dependent variable .

Test-specific

Once you know the statistical test you’ll be using, you can write your hypotheses in a more precise and mathematical way specific to the test you chose. The table below provides template sentences for common statistical tests.

( )
test 

with two groups

The mean dependent variable does not differ between group 1 (µ ) and group 2 (µ ) in the population; µ = µ . The mean dependent variable differs between group 1 (µ ) and group 2 (µ ) in the population; µ ≠ µ .
with three groups The mean dependent variable does not differ between group 1 (µ ), group 2 (µ ), and group 3 (µ ) in the population; µ = µ = µ . The mean dependent variable of group 1 (µ ), group 2 (µ ), and group 3 (µ ) are not all equal in the population.
There is no correlation between independent variable and dependent variable in the population; ρ = 0. There is a correlation between independent variable and dependent variable in the population; ρ ≠ 0.
There is no relationship between independent variable and dependent variable in the population; β = 0. There is a relationship between independent variable and dependent variable in the population; β ≠ 0.
Two-proportions test The dependent variable expressed as a proportion does not differ between group 1 ( ) and group 2 ( ) in the population; = . The dependent variable expressed as a proportion differs between group 1 ( ) and group 2 ( ) in the population; ≠ .

Note: The template sentences above assume that you’re performing one-tailed tests . One-tailed tests are appropriate for most studies.

The null hypothesis is often abbreviated as H 0 . When the null hypothesis is written using mathematical symbols, it always includes an equality symbol (usually =, but sometimes ≥ or ≤).

The alternative hypothesis is often abbreviated as H a or H 1 . When the alternative hypothesis is written using mathematical symbols, it always includes an inequality symbol (usually ≠, but sometimes < or >).

A research hypothesis is your proposed answer to your research question. The research hypothesis usually includes an explanation (‘ x affects y because …’).

A statistical hypothesis, on the other hand, is a mathematical statement about a population parameter. Statistical hypotheses always come in pairs: the null and alternative hypotheses. In a well-designed study , the statistical hypotheses correspond logically to the research hypothesis.

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Turney, S. (2022, December 06). Null and Alternative Hypotheses | Definitions & Examples. Scribbr. Retrieved 12 August 2024, from https://www.scribbr.co.uk/stats/null-and-alternative-hypothesis/

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Writing Null Hypotheses in Research and Statistics

Last Updated: January 17, 2024 Fact Checked

This article was co-authored by Joseph Quinones and by wikiHow staff writer, Jennifer Mueller, JD . Joseph Quinones is a High School Physics Teacher working at South Bronx Community Charter High School. Joseph specializes in astronomy and astrophysics and is interested in science education and science outreach, currently practicing ways to make physics accessible to more students with the goal of bringing more students of color into the STEM fields. He has experience working on Astrophysics research projects at the Museum of Natural History (AMNH). Joseph recieved his Bachelor's degree in Physics from Lehman College and his Masters in Physics Education from City College of New York (CCNY). He is also a member of a network called New York City Men Teach. There are 7 references cited in this article, which can be found at the bottom of the page. This article has been fact-checked, ensuring the accuracy of any cited facts and confirming the authority of its sources. This article has been viewed 28,500 times.

Are you working on a research project and struggling with how to write a null hypothesis? Well, you've come to the right place! Start by recognizing that the basic definition of "null" is "none" or "zero"—that's your biggest clue as to what a null hypothesis should say. Keep reading to learn everything you need to know about the null hypothesis, including how it relates to your research question and your alternative hypothesis as well as how to use it in different types of studies.

Things You Should Know

  • Write a research null hypothesis as a statement that the studied variables have no relationship to each other, or that there's no difference between 2 groups.

{\displaystyle \mu _{1}=\mu _{2}}

  • Adjust the format of your null hypothesis to match the statistical method you used to test it, such as using "mean" if you're comparing the mean between 2 groups.

What is a null hypothesis?

A null hypothesis states that there's no relationship between 2 variables.

  • Research hypothesis: States in plain language that there's no relationship between the 2 variables or there's no difference between the 2 groups being studied.
  • Statistical hypothesis: States the predicted outcome of statistical analysis through a mathematical equation related to the statistical method you're using.

Examples of Null Hypotheses

Step 1 Research question:

Null Hypothesis vs. Alternative Hypothesis

Step 1 Null hypotheses and alternative hypotheses are mutually exclusive.

  • For example, your alternative hypothesis could state a positive correlation between 2 variables while your null hypothesis states there's no relationship. If there's a negative correlation, then both hypotheses are false.

Step 2 Proving the null hypothesis false is a precursor to proving the alternative.

  • You need additional data or evidence to show that your alternative hypothesis is correct—proving the null hypothesis false is just the first step.
  • In smaller studies, sometimes it's enough to show that there's some relationship and your hypothesis could be correct—you can leave the additional proof as an open question for other researchers to tackle.

How do I test a null hypothesis?

Use statistical methods on collected data to test the null hypothesis.

  • Group means: Compare the mean of the variable in your sample with the mean of the variable in the general population. [6] X Research source
  • Group proportions: Compare the proportion of the variable in your sample with the proportion of the variable in the general population. [7] X Research source
  • Correlation: Correlation analysis looks at the relationship between 2 variables—specifically, whether they tend to happen together. [8] X Research source
  • Regression: Regression analysis reveals the correlation between 2 variables while also controlling for the effect of other, interrelated variables. [9] X Research source

Templates for Null Hypotheses

Step 1 Group means

  • Research null hypothesis: There is no difference in the mean [dependent variable] between [group 1] and [group 2].

{\displaystyle \mu _{1}+\mu _{2}=0}

  • Research null hypothesis: The proportion of [dependent variable] in [group 1] and [group 2] is the same.

{\displaystyle p_{1}=p_{2}}

  • Research null hypothesis: There is no correlation between [independent variable] and [dependent variable] in the population.

\rho =0

  • Research null hypothesis: There is no relationship between [independent variable] and [dependent variable] in the population.

{\displaystyle \beta =0}

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  • ↑ https://online.stat.psu.edu/stat100/lesson/10/10.1
  • ↑ https://online.stat.psu.edu/stat501/lesson/2/2.12
  • ↑ https://support.minitab.com/en-us/minitab/21/help-and-how-to/statistics/basic-statistics/supporting-topics/basics/null-and-alternative-hypotheses/
  • ↑ https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5635437/
  • ↑ https://online.stat.psu.edu/statprogram/reviews/statistical-concepts/hypothesis-testing
  • ↑ https://education.arcus.chop.edu/null-hypothesis-testing/
  • ↑ https://sphweb.bumc.bu.edu/otlt/mph-modules/bs/bs704_hypothesistest-means-proportions/bs704_hypothesistest-means-proportions_print.html

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Null Hypothesis Examples

Null Hypothesis Example

The null hypothesis (H 0 ) is the hypothesis that states there is no statistical difference between two sample sets. In other words, it assumes the independent variable does not have an effect on the dependent variable in a scientific experiment .

The null hypothesis is the most powerful type of hypothesis in the scientific method because it’s the easiest one to test with a high confidence level using statistics. If the null hypothesis is accepted, then it’s evidence any observed differences between two experiment groups are due to random chance. If the null hypothesis is rejected, then it’s strong evidence there is a true difference between test sets or that the independent variable affects the dependent variable.

  • The null hypothesis is a nullifiable hypothesis. A researcher seeks to reject it because this result strongly indicates observed differences are real and not just due to chance.
  • The null hypothesis may be accepted or rejected, but not proven. There is always a level of confidence in the outcome.

What Is the Null Hypothesis?

The null hypothesis is written as H 0 , which is read as H-zero, H-nought, or H-null. It is associated with another hypothesis, called the alternate or alternative hypothesis H A or H 1 . When the null hypothesis and alternate hypothesis are written mathematically, they cover all possible outcomes of an experiment.

An experimenter tests the null hypothesis with a statistical analysis called a significance test. The significance test determines the likelihood that the results of the test are not due to chance. Usually, a researcher uses a confidence level of 95% or 99% (p-value of 0.05 or 0.01). But, even if the confidence in the test is high, there is always a small chance the outcome is incorrect. This means you can’t prove a null hypothesis. It’s also a good reason why it’s important to repeat experiments.

Exact and Inexact Null Hypothesis

The most common type of null hypothesis assumes no difference between two samples or groups or no measurable effect of a treatment. This is the exact hypothesis . If you’re asked to state a null hypothesis for a science class, this is the one to write. It is the easiest type of hypothesis to test and is the only one accepted for certain types of analysis. Examples include:

There is no difference between two groups H 0 : μ 1  = μ 2 (where H 0  = the null hypothesis, μ 1  = the mean of population 1, and μ 2  = the mean of population 2)

Both groups have value of 100 (or any number or quality) H 0 : μ = 100

However, sometimes a researcher may test an inexact hypothesis . This type of hypothesis specifies ranges or intervals. Examples include:

Recovery time from a treatment is the same or worse than a placebo: H 0 : μ ≥ placebo time

There is a 5% or less difference between two groups: H 0 : 95 ≤ μ ≤ 105

An inexact hypothesis offers “directionality” about a phenomenon. For example, an exact hypothesis can indicate whether or not a treatment has an effect, while an inexact hypothesis can tell whether an effect is positive of negative. However, an inexact hypothesis may be harder to test and some scientists and statisticians disagree about whether it’s a true null hypothesis .

How to State the Null Hypothesis

To state the null hypothesis, first state what you expect the experiment to show. Then, rephrase the statement in a form that assumes there is no relationship between the variables or that a treatment has no effect.

Example: A researcher tests whether a new drug speeds recovery time from a certain disease. The average recovery time without treatment is 3 weeks.

  • State the goal of the experiment: “I hope the average recovery time with the new drug will be less than 3 weeks.”
  • Rephrase the hypothesis to assume the treatment has no effect: “If the drug doesn’t shorten recovery time, then the average time will be 3 weeks or longer.” Mathematically: H 0 : μ ≥ 3

This null hypothesis (inexact hypothesis) covers both the scenario in which the drug has no effect and the one in which the drugs makes the recovery time longer. The alternate hypothesis is that average recovery time will be less than three weeks:

H A : μ < 3

Of course, the researcher could test the no-effect hypothesis (exact null hypothesis): H 0 : μ = 3

The danger of testing this hypothesis is that rejecting it only implies the drug affected recovery time (not whether it made it better or worse). This is because the alternate hypothesis is:

H A : μ ≠ 3 (which includes μ <3 and μ >3)

Even though the no-effect null hypothesis yields less information, it’s used because it’s easier to test using statistics. Basically, testing whether something is unchanged/changed is easier than trying to quantify the nature of the change.

Remember, a researcher hopes to reject the null hypothesis because this supports the alternate hypothesis. Also, be sure the null and alternate hypothesis cover all outcomes. Finally, remember a simple true/false, equal/unequal, yes/no exact hypothesis is easier to test than a more complex inexact hypothesis.

Does chewing willow bark relieve pain?Pain relief is the same compared with a . (exact)
Pain relief after chewing willow bark is the same or worse versus taking a placebo. (inexact)
Pain relief is different compared with a placebo. (exact)
Pain relief is better compared to a placebo. (inexact)
Do cats care about the shape of their food?Cats show no food preference based on shape. (exact)Cat show a food preference based on shape. (exact)
Do teens use mobile devices more than adults?Teens and adults use mobile devices the same amount. (exact)
Teens use mobile devices less than or equal to adults. (inexact)
Teens and adults used mobile devices different amounts. (exact)
Teens use mobile devices more than adults. (inexact)
Does the color of light influence plant growth?The color of light has no effect on plant growth. (exact)The color of light affects plant growth. (exact)
  • Adèr, H. J.; Mellenbergh, G. J. & Hand, D. J. (2007).  Advising on Research Methods: A Consultant’s Companion . Huizen, The Netherlands: Johannes van Kessel Publishing. ISBN  978-90-79418-01-5 .
  • Cox, D. R. (2006).  Principles of Statistical Inference . Cambridge University Press. ISBN  978-0-521-68567-2 .
  • Everitt, Brian (1998).  The Cambridge Dictionary of Statistics . Cambridge, UK New York: Cambridge University Press. ISBN 978-0521593465.
  • Weiss, Neil A. (1999).  Introductory Statistics  (5th ed.). ISBN 9780201598773.

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  • How to Write a Strong Hypothesis | Steps & Examples

How to Write a Strong Hypothesis | Steps & Examples

Published on May 6, 2022 by Shona McCombes . Revised on November 20, 2023.

A hypothesis is a statement that can be tested by scientific research. If you want to test a relationship between two or more variables, you need to write hypotheses before you start your experiment or data collection .

Example: Hypothesis

Daily apple consumption leads to fewer doctor’s visits.

Table of contents

What is a hypothesis, developing a hypothesis (with example), hypothesis examples, other interesting articles, frequently asked questions about writing hypotheses.

A hypothesis states your predictions about what your research will find. It is a tentative answer to your research question that has not yet been tested. For some research projects, you might have to write several hypotheses that address different aspects of your research question.

A hypothesis is not just a guess – it should be based on existing theories and knowledge. It also has to be testable, which means you can support or refute it through scientific research methods (such as experiments, observations and statistical analysis of data).

Variables in hypotheses

Hypotheses propose a relationship between two or more types of variables .

  • An independent variable is something the researcher changes or controls.
  • A dependent variable is something the researcher observes and measures.

If there are any control variables , extraneous variables , or confounding variables , be sure to jot those down as you go to minimize the chances that research bias  will affect your results.

In this example, the independent variable is exposure to the sun – the assumed cause . The dependent variable is the level of happiness – the assumed effect .

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Step 1. ask a question.

Writing a hypothesis begins with a research question that you want to answer. The question should be focused, specific, and researchable within the constraints of your project.

Step 2. Do some preliminary research

Your initial answer to the question should be based on what is already known about the topic. Look for theories and previous studies to help you form educated assumptions about what your research will find.

At this stage, you might construct a conceptual framework to ensure that you’re embarking on a relevant topic . This can also help you identify which variables you will study and what you think the relationships are between them. Sometimes, you’ll have to operationalize more complex constructs.

Step 3. Formulate your hypothesis

Now you should have some idea of what you expect to find. Write your initial answer to the question in a clear, concise sentence.

4. Refine your hypothesis

You need to make sure your hypothesis is specific and testable. There are various ways of phrasing a hypothesis, but all the terms you use should have clear definitions, and the hypothesis should contain:

  • The relevant variables
  • The specific group being studied
  • The predicted outcome of the experiment or analysis

5. Phrase your hypothesis in three ways

To identify the variables, you can write a simple prediction in  if…then form. The first part of the sentence states the independent variable and the second part states the dependent variable.

In academic research, hypotheses are more commonly phrased in terms of correlations or effects, where you directly state the predicted relationship between variables.

If you are comparing two groups, the hypothesis can state what difference you expect to find between them.

6. Write a null hypothesis

If your research involves statistical hypothesis testing , you will also have to write a null hypothesis . The null hypothesis is the default position that there is no association between the variables. The null hypothesis is written as H 0 , while the alternative hypothesis is H 1 or H a .

  • H 0 : The number of lectures attended by first-year students has no effect on their final exam scores.
  • H 1 : The number of lectures attended by first-year students has a positive effect on their final exam scores.
Research question Hypothesis Null hypothesis
What are the health benefits of eating an apple a day? Increasing apple consumption in over-60s will result in decreasing frequency of doctor’s visits. Increasing apple consumption in over-60s will have no effect on frequency of doctor’s visits.
Which airlines have the most delays? Low-cost airlines are more likely to have delays than premium airlines. Low-cost and premium airlines are equally likely to have delays.
Can flexible work arrangements improve job satisfaction? Employees who have flexible working hours will report greater job satisfaction than employees who work fixed hours. There is no relationship between working hour flexibility and job satisfaction.
How effective is high school sex education at reducing teen pregnancies? Teenagers who received sex education lessons throughout high school will have lower rates of unplanned pregnancy teenagers who did not receive any sex education. High school sex education has no effect on teen pregnancy rates.
What effect does daily use of social media have on the attention span of under-16s? There is a negative between time spent on social media and attention span in under-16s. There is no relationship between social media use and attention span in under-16s.

If you want to know more about the research process , methodology , research bias , or statistics , make sure to check out some of our other articles with explanations and examples.

  • Sampling methods
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A hypothesis is not just a guess — it should be based on existing theories and knowledge. It also has to be testable, which means you can support or refute it through scientific research methods (such as experiments, observations and statistical analysis of data).

Null and alternative hypotheses are used in statistical hypothesis testing . The null hypothesis of a test always predicts no effect or no relationship between variables, while the alternative hypothesis states your research prediction of an effect or relationship.

Hypothesis testing is a formal procedure for investigating our ideas about the world using statistics. It is used by scientists to test specific predictions, called hypotheses , by calculating how likely it is that a pattern or relationship between variables could have arisen by chance.

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Null Hypothesis Definition and Examples

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In a scientific experiment, the null hypothesis is the proposition that there is no effect or no relationship between phenomena or populations. If the null hypothesis is true, any observed difference in phenomena or populations would be due to sampling error (random chance) or experimental error. The null hypothesis is useful because it can be tested and found to be false, which then implies that there is a relationship between the observed data. It may be easier to think of it as a nullifiable hypothesis or one that the researcher seeks to nullify. The null hypothesis is also known as the H 0, or no-difference hypothesis.

The alternate hypothesis, H A or H 1 , proposes that observations are influenced by a non-random factor. In an experiment, the alternate hypothesis suggests that the experimental or independent variable has an effect on the dependent variable .

How to State a Null Hypothesis

There are two ways to state a null hypothesis. One is to state it as a declarative sentence, and the other is to present it as a mathematical statement.

For example, say a researcher suspects that exercise is correlated to weight loss, assuming diet remains unchanged. The average length of time to achieve a certain amount of weight loss is six weeks when a person works out five times a week. The researcher wants to test whether weight loss takes longer to occur if the number of workouts is reduced to three times a week.

The first step to writing the null hypothesis is to find the (alternate) hypothesis. In a word problem like this, you're looking for what you expect to be the outcome of the experiment. In this case, the hypothesis is "I expect weight loss to take longer than six weeks."

This can be written mathematically as: H 1 : μ > 6

In this example, μ is the average.

Now, the null hypothesis is what you expect if this hypothesis does not happen. In this case, if weight loss isn't achieved in greater than six weeks, then it must occur at a time equal to or less than six weeks. This can be written mathematically as:

H 0 : μ ≤ 6

The other way to state the null hypothesis is to make no assumption about the outcome of the experiment. In this case, the null hypothesis is simply that the treatment or change will have no effect on the outcome of the experiment. For this example, it would be that reducing the number of workouts would not affect the time needed to achieve weight loss:

H 0 : μ = 6

Null Hypothesis Examples

"Hyperactivity is unrelated to eating sugar " is an example of a null hypothesis. If the hypothesis is tested and found to be false, using statistics, then a connection between hyperactivity and sugar ingestion may be indicated. A significance test is the most common statistical test used to establish confidence in a null hypothesis.

Another example of a null hypothesis is "Plant growth rate is unaffected by the presence of cadmium in the soil ." A researcher could test the hypothesis by measuring the growth rate of plants grown in a medium lacking cadmium, compared with the growth rate of plants grown in mediums containing different amounts of cadmium. Disproving the null hypothesis would set the groundwork for further research into the effects of different concentrations of the element in soil.

Why Test a Null Hypothesis?

You may be wondering why you would want to test a hypothesis just to find it false. Why not just test an alternate hypothesis and find it true? The short answer is that it is part of the scientific method. In science, propositions are not explicitly "proven." Rather, science uses math to determine the probability that a statement is true or false. It turns out it's much easier to disprove a hypothesis than to positively prove one. Also, while the null hypothesis may be simply stated, there's a good chance the alternate hypothesis is incorrect.

For example, if your null hypothesis is that plant growth is unaffected by duration of sunlight, you could state the alternate hypothesis in several different ways. Some of these statements might be incorrect. You could say plants are harmed by more than 12 hours of sunlight or that plants need at least three hours of sunlight, etc. There are clear exceptions to those alternate hypotheses, so if you test the wrong plants, you could reach the wrong conclusion. The null hypothesis is a general statement that can be used to develop an alternate hypothesis, which may or may not be correct.

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Formulating a Null Hypothesis: Key Elements to Consider

Formulating a Null Hypothesis: Key Elements to Consider

The concept of the null hypothesis is a cornerstone of statistical hypothesis testing. In the article 'Formulating a Null Hypothesis: Key Elements to Consider,' we delve into what a null hypothesis is, why it's crucial for research, and how to properly formulate one. This article offers a comprehensive guide for researchers and students alike, providing the necessary tools to craft a null hypothesis that effectively sets the stage for rigorous scientific inquiry.

Key Takeaways

  • A null hypothesis (H0) is a statement that there is no effect or no difference, and it serves as the starting point for statistical testing.
  • Formulating a null hypothesis involves defining a clear and concise research question, stating the hypothesis in a way that allows for empirical testing, and considering the potential for Type I errors.
  • Evaluating a null hypothesis requires understanding its role in research design, recognizing common misconceptions, and being aware of the challenges in crafting a hypothesis that is both testable and meaningful.

Understanding the Null Hypothesis

Defining the null hypothesis.

The null hypothesis , often represented as H0, is the default assumption that there is no effect or no difference in the context of scientific research. It posits a position of neutrality, suggesting that any observed variations in data are due to chance rather than a specific cause or intervention. Formulating a null hypothesis is a foundational step in hypothesis testing , where it is contrasted with an alternative hypothesis (Ha) that predicts an effect or difference.

Importance of the Null Hypothesis in Research

In the research process, the null hypothesis plays a critical role as it provides a benchmark against which the validity of the study's findings is assessed. It is essential for identifying variables, crafting clear hypotheses, and conducting targeted research that advances scientific knowledge. The research process involves revisiting initial assumptions , evaluating the design, considering alternative explanations, adjusting methodology, and addressing limitations when faced with contradictory data.

Common Misconceptions and Clarifications

There are several misconceptions about the null hypothesis that can lead to confusion. One common error is the belief that a failure to reject the null hypothesis is evidence of no effect, which is not necessarily true. It may simply indicate insufficient evidence to support the alternative hypothesis. Another misunderstanding is equating the null hypothesis with the belief that there is no relationship between variables, which overlooks the fact that it is a tool for statistical testing, not a definitive statement about reality.

Crafting the Null Hypothesis

Steps for formulating a null hypothesis.

When you're learning how to write a thesis or a research paper, formulating a null hypothesis is a critical step. Begin by clearly defining the variables or groups you are studying. Next, state the null hypothesis as a position of no effect or no difference, implying that any observed effect is due to chance. Ensure that your hypothesis is testable and measurable, and consider any potential limitations or biases that could affect the results.

Examples of Null Hypotheses in Various Disciplines

In various academic fields, the null hypothesis takes on different forms. For instance, in psychology, a null hypothesis might state that a new therapy has no effect on depression levels compared to the standard treatment. In ecology, it could assert that there is no significant difference in biodiversity between two protected areas. These examples illustrate how the null hypothesis is tailored to the specific research question and discipline.

Evaluating the Null Hypothesis: Considerations and Challenges

Evaluating the null hypothesis involves selecting appropriate statistical tests and determining the significance level. It's essential to understand the difference between statistical and practical significance . Writing anxiety can arise during this phase, especially when interpreting complex data. However, a systematic approach to hypothesis testing can help alleviate this stress and lead to meaningful research conclusions.

Embarking on the journey of thesis writing can be daunting, but with Research Rebels , you're not alone. Our step-by-step Thesis Action Plan is designed to transform your anxiety and uncertainty into confidence and clarity. From crafting the perfect Null Hypothesis to navigating complex research methodologies, we've got you covered. Don't let sleepless nights hinder your academic success. Visit our website now to claim your special offer and take the first step towards a stress-free thesis experience.

In conclusion, formulating a null hypothesis is a fundamental step in the research process, serving as a critical benchmark against which scientific evidence is measured. A well-constructed null hypothesis provides clarity and direction, allowing for rigorous testing and meaningful interpretation of results. It is essential to articulate the null hypothesis with precision, ensuring it is testable, falsifiable, and appropriately framed to reflect the absence of an effect or relationship. By carefully considering the key elements discussed in this article, researchers can establish a robust foundation for their empirical inquiries, ultimately contributing to the advancement of knowledge within their respective fields.

Frequently Asked Questions

What is the null hypothesis in research.

The null hypothesis (H0) is a statement in research that suggests there is no significant effect or difference between certain populations, conditions, or variables. It is the default assumption that there is no relationship or impact, and it is tested to determine if there is evidence to support an alternative hypothesis.

How do you formulate a null hypothesis?

To formulate a null hypothesis, first identify the research question or problem. Then, state the null hypothesis in a way that it asserts no effect or no difference between groups or variables. It should be clear, specific, and testable, often structured as H0: parameter = value (e.g., H0: μ1 = μ2).

What are common challenges in evaluating the null hypothesis?

Challenges in evaluating the null hypothesis include ensuring the study design and data collection methods are appropriate, selecting the correct statistical test, interpreting the results correctly, and understanding the potential for Type I (false positive) and Type II (false negative) errors.

Crafting a Null Hypothesis: A Guide to Writing it Right

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Null Hypothesis Definition and Examples, How to State

What is the null hypothesis, how to state the null hypothesis, null hypothesis overview.

null hypothesis in research paper example

Why is it Called the “Null”?

The word “null” in this context means that it’s a commonly accepted fact that researchers work to nullify . It doesn’t mean that the statement is null (i.e. amounts to nothing) itself! (Perhaps the term should be called the “nullifiable hypothesis” as that might cause less confusion).

Why Do I need to Test it? Why not just prove an alternate one?

The short answer is, as a scientist, you are required to ; It’s part of the scientific process. Science uses a battery of processes to prove or disprove theories, making sure than any new hypothesis has no flaws. Including both a null and an alternate hypothesis is one safeguard to ensure your research isn’t flawed. Not including the null hypothesis in your research is considered very bad practice by the scientific community. If you set out to prove an alternate hypothesis without considering it, you are likely setting yourself up for failure. At a minimum, your experiment will likely not be taken seriously.

null hypothesis

  • Null hypothesis : H 0 : The world is flat.
  • Alternate hypothesis: The world is round.

Several scientists, including Copernicus , set out to disprove the null hypothesis. This eventually led to the rejection of the null and the acceptance of the alternate. Most people accepted it — the ones that didn’t created the Flat Earth Society !. What would have happened if Copernicus had not disproved the it and merely proved the alternate? No one would have listened to him. In order to change people’s thinking, he first had to prove that their thinking was wrong .

How to State the Null Hypothesis from a Word Problem

You’ll be asked to convert a word problem into a hypothesis statement in statistics that will include a null hypothesis and an alternate hypothesis . Breaking your problem into a few small steps makes these problems much easier to handle.

how to state the null hypothesis

Step 2: Convert the hypothesis to math . Remember that the average is sometimes written as μ.

H 1 : μ > 8.2

Broken down into (somewhat) English, that’s H 1 (The hypothesis): μ (the average) > (is greater than) 8.2

Step 3: State what will happen if the hypothesis doesn’t come true. If the recovery time isn’t greater than 8.2 weeks, there are only two possibilities, that the recovery time is equal to 8.2 weeks or less than 8.2 weeks.

H 0 : μ ≤ 8.2

Broken down again into English, that’s H 0 (The null hypothesis): μ (the average) ≤ (is less than or equal to) 8.2

How to State the Null Hypothesis: Part Two

But what if the researcher doesn’t have any idea what will happen.

Example Problem: A researcher is studying the effects of radical exercise program on knee surgery patients. There is a good chance the therapy will improve recovery time, but there’s also the possibility it will make it worse. Average recovery times for knee surgery patients is 8.2 weeks. 

Step 1: State what will happen if the experiment doesn’t make any difference. That’s the null hypothesis–that nothing will happen. In this experiment, if nothing happens, then the recovery time will stay at 8.2 weeks.

H 0 : μ = 8.2

Broken down into English, that’s H 0 (The null hypothesis): μ (the average) = (is equal to) 8.2

Step 2: Figure out the alternate hypothesis . The alternate hypothesis is the opposite of the null hypothesis. In other words, what happens if our experiment makes a difference?

H 1 : μ ≠ 8.2

In English again, that’s H 1 (The  alternate hypothesis): μ (the average) ≠ (is not equal to) 8.2

That’s How to State the Null Hypothesis!

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Video transcript

What is The Null Hypothesis & When Do You Reject The Null Hypothesis

Julia Simkus

Editor at Simply Psychology

BA (Hons) Psychology, Princeton University

Julia Simkus is a graduate of Princeton University with a Bachelor of Arts in Psychology. She is currently studying for a Master's Degree in Counseling for Mental Health and Wellness in September 2023. Julia's research has been published in peer reviewed journals.

Learn about our Editorial Process

Saul McLeod, PhD

Editor-in-Chief for Simply Psychology

BSc (Hons) Psychology, MRes, PhD, University of Manchester

Saul McLeod, PhD., is a qualified psychology teacher with over 18 years of experience in further and higher education. He has been published in peer-reviewed journals, including the Journal of Clinical Psychology.

Olivia Guy-Evans, MSc

Associate Editor for Simply Psychology

BSc (Hons) Psychology, MSc Psychology of Education

Olivia Guy-Evans is a writer and associate editor for Simply Psychology. She has previously worked in healthcare and educational sectors.

On This Page:

A null hypothesis is a statistical concept suggesting no significant difference or relationship between measured variables. It’s the default assumption unless empirical evidence proves otherwise.

The null hypothesis states no relationship exists between the two variables being studied (i.e., one variable does not affect the other).

The null hypothesis is the statement that a researcher or an investigator wants to disprove.

Testing the null hypothesis can tell you whether your results are due to the effects of manipulating ​ the dependent variable or due to random chance. 

How to Write a Null Hypothesis

Null hypotheses (H0) start as research questions that the investigator rephrases as statements indicating no effect or relationship between the independent and dependent variables.

It is a default position that your research aims to challenge or confirm.

For example, if studying the impact of exercise on weight loss, your null hypothesis might be:

There is no significant difference in weight loss between individuals who exercise daily and those who do not.

Examples of Null Hypotheses

Research QuestionNull Hypothesis
Do teenagers use cell phones more than adults?Teenagers and adults use cell phones the same amount.
Do tomato plants exhibit a higher rate of growth when planted in compost rather than in soil?Tomato plants show no difference in growth rates when planted in compost rather than soil.
Does daily meditation decrease the incidence of depression?Daily meditation does not decrease the incidence of depression.
Does daily exercise increase test performance?There is no relationship between daily exercise time and test performance.
Does the new vaccine prevent infections?The vaccine does not affect the infection rate.
Does flossing your teeth affect the number of cavities?Flossing your teeth has no effect on the number of cavities.

When Do We Reject The Null Hypothesis? 

We reject the null hypothesis when the data provide strong enough evidence to conclude that it is likely incorrect. This often occurs when the p-value (probability of observing the data given the null hypothesis is true) is below a predetermined significance level.

If the collected data does not meet the expectation of the null hypothesis, a researcher can conclude that the data lacks sufficient evidence to back up the null hypothesis, and thus the null hypothesis is rejected. 

Rejecting the null hypothesis means that a relationship does exist between a set of variables and the effect is statistically significant ( p > 0.05).

If the data collected from the random sample is not statistically significance , then the null hypothesis will be accepted, and the researchers can conclude that there is no relationship between the variables. 

You need to perform a statistical test on your data in order to evaluate how consistent it is with the null hypothesis. A p-value is one statistical measurement used to validate a hypothesis against observed data.

Calculating the p-value is a critical part of null-hypothesis significance testing because it quantifies how strongly the sample data contradicts the null hypothesis.

The level of statistical significance is often expressed as a  p  -value between 0 and 1. The smaller the p-value, the stronger the evidence that you should reject the null hypothesis.

Probability and statistical significance in ab testing. Statistical significance in a b experiments

Usually, a researcher uses a confidence level of 95% or 99% (p-value of 0.05 or 0.01) as general guidelines to decide if you should reject or keep the null.

When your p-value is less than or equal to your significance level, you reject the null hypothesis.

In other words, smaller p-values are taken as stronger evidence against the null hypothesis. Conversely, when the p-value is greater than your significance level, you fail to reject the null hypothesis.

In this case, the sample data provides insufficient data to conclude that the effect exists in the population.

Because you can never know with complete certainty whether there is an effect in the population, your inferences about a population will sometimes be incorrect.

When you incorrectly reject the null hypothesis, it’s called a type I error. When you incorrectly fail to reject it, it’s called a type II error.

Why Do We Never Accept The Null Hypothesis?

The reason we do not say “accept the null” is because we are always assuming the null hypothesis is true and then conducting a study to see if there is evidence against it. And, even if we don’t find evidence against it, a null hypothesis is not accepted.

A lack of evidence only means that you haven’t proven that something exists. It does not prove that something doesn’t exist. 

It is risky to conclude that the null hypothesis is true merely because we did not find evidence to reject it. It is always possible that researchers elsewhere have disproved the null hypothesis, so we cannot accept it as true, but instead, we state that we failed to reject the null. 

One can either reject the null hypothesis, or fail to reject it, but can never accept it.

Why Do We Use The Null Hypothesis?

We can never prove with 100% certainty that a hypothesis is true; We can only collect evidence that supports a theory. However, testing a hypothesis can set the stage for rejecting or accepting this hypothesis within a certain confidence level.

The null hypothesis is useful because it can tell us whether the results of our study are due to random chance or the manipulation of a variable (with a certain level of confidence).

A null hypothesis is rejected if the measured data is significantly unlikely to have occurred and a null hypothesis is accepted if the observed outcome is consistent with the position held by the null hypothesis.

Rejecting the null hypothesis sets the stage for further experimentation to see if a relationship between two variables exists. 

Hypothesis testing is a critical part of the scientific method as it helps decide whether the results of a research study support a particular theory about a given population. Hypothesis testing is a systematic way of backing up researchers’ predictions with statistical analysis.

It helps provide sufficient statistical evidence that either favors or rejects a certain hypothesis about the population parameter. 

Purpose of a Null Hypothesis 

  • The primary purpose of the null hypothesis is to disprove an assumption. 
  • Whether rejected or accepted, the null hypothesis can help further progress a theory in many scientific cases.
  • A null hypothesis can be used to ascertain how consistent the outcomes of multiple studies are.

Do you always need both a Null Hypothesis and an Alternative Hypothesis?

The null (H0) and alternative (Ha or H1) hypotheses are two competing claims that describe the effect of the independent variable on the dependent variable. They are mutually exclusive, which means that only one of the two hypotheses can be true. 

While the null hypothesis states that there is no effect in the population, an alternative hypothesis states that there is statistical significance between two variables. 

The goal of hypothesis testing is to make inferences about a population based on a sample. In order to undertake hypothesis testing, you must express your research hypothesis as a null and alternative hypothesis. Both hypotheses are required to cover every possible outcome of the study. 

What is the difference between a null hypothesis and an alternative hypothesis?

The alternative hypothesis is the complement to the null hypothesis. The null hypothesis states that there is no effect or no relationship between variables, while the alternative hypothesis claims that there is an effect or relationship in the population.

It is the claim that you expect or hope will be true. The null hypothesis and the alternative hypothesis are always mutually exclusive, meaning that only one can be true at a time.

What are some problems with the null hypothesis?

One major problem with the null hypothesis is that researchers typically will assume that accepting the null is a failure of the experiment. However, accepting or rejecting any hypothesis is a positive result. Even if the null is not refuted, the researchers will still learn something new.

Why can a null hypothesis not be accepted?

We can either reject or fail to reject a null hypothesis, but never accept it. If your test fails to detect an effect, this is not proof that the effect doesn’t exist. It just means that your sample did not have enough evidence to conclude that it exists.

We can’t accept a null hypothesis because a lack of evidence does not prove something that does not exist. Instead, we fail to reject it.

Failing to reject the null indicates that the sample did not provide sufficient enough evidence to conclude that an effect exists.

If the p-value is greater than the significance level, then you fail to reject the null hypothesis.

Is a null hypothesis directional or non-directional?

A hypothesis test can either contain an alternative directional hypothesis or a non-directional alternative hypothesis. A directional hypothesis is one that contains the less than (“<“) or greater than (“>”) sign.

A nondirectional hypothesis contains the not equal sign (“≠”).  However, a null hypothesis is neither directional nor non-directional.

A null hypothesis is a prediction that there will be no change, relationship, or difference between two variables.

The directional hypothesis or nondirectional hypothesis would then be considered alternative hypotheses to the null hypothesis.

Gill, J. (1999). The insignificance of null hypothesis significance testing.  Political research quarterly ,  52 (3), 647-674.

Krueger, J. (2001). Null hypothesis significance testing: On the survival of a flawed method.  American Psychologist ,  56 (1), 16.

Masson, M. E. (2011). A tutorial on a practical Bayesian alternative to null-hypothesis significance testing.  Behavior research methods ,  43 , 679-690.

Nickerson, R. S. (2000). Null hypothesis significance testing: a review of an old and continuing controversy.  Psychological methods ,  5 (2), 241.

Rozeboom, W. W. (1960). The fallacy of the null-hypothesis significance test.  Psychological bulletin ,  57 (5), 416.

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Null Hypothesis

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null hypothesis in research paper example

  • Tom Booth 3 ,
  • Alex Doumas 3 &
  • Aja Louise Murray 4  

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In formal hypothesis testing, the null hypothesis ( H 0 ) is the hypothesis assumed to be true in the population and which gives rise to the sampling distribution of the test statistic in question (Hays 1994 ). The critical feature of the null hypothesis across hypothesis testing frameworks is that it is stated with enough precision that it can be tested.

Introduction

A hypothesis is a statement or explanation about the nature or causes of some phenomena of interest. In the process of scientific study, we can distinguish two forms of hypotheses. A research hypothesis poses the question of interest, and if well stated, will include the variables under study and the expected relationship between them. A statistical hypothesis translates the research hypothesis into a mathematically precise, statistically testable statement concerning the assumed value of a parameter of interest in the population. The null hypothesis is an example of a statistical hypothesis.

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Fisher, R. (1925). Statistical methods for research workers (1st ed.). Edinburgh: Oliver and Boyd.

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Gigerenzer, G. (2004). Mindless statistics. The Journal of Socio-Economics, 33 , 587–606.

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Hays, W. L. (1994). Statistics (5th ed.). Belmont: Wadsworth.

Neyman, J., & Pearson, E. S. (1933). On the problem of the most efficient tests of statistical hypotheses. Philosophical Transactions of the Royal Society of London, Series A, 231 , 289–337.

Szucs, D., & Ioannidis, J. P. A. (2016). When null hypothesis significance testing is unsuitable for research: A reassessment. bioRxiv . https://doi.org/10.1101/095570 .

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Booth, T., Doumas, A., Murray, A.L. (2020). Null Hypothesis. In: Zeigler-Hill, V., Shackelford, T.K. (eds) Encyclopedia of Personality and Individual Differences. Springer, Cham. https://doi.org/10.1007/978-3-319-24612-3_1335

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10.1 - setting the hypotheses: examples.

A significance test examines whether the null hypothesis provides a plausible explanation of the data. The null hypothesis itself does not involve the data. It is a statement about a parameter (a numerical characteristic of the population). These population values might be proportions or means or differences between means or proportions or correlations or odds ratios or any other numerical summary of the population. The alternative hypothesis is typically the research hypothesis of interest. Here are some examples.

Example 10.2: Hypotheses with One Sample of One Categorical Variable Section  

About 10% of the human population is left-handed. Suppose a researcher at Penn State speculates that students in the College of Arts and Architecture are more likely to be left-handed than people found in the general population. We only have one sample since we will be comparing a population proportion based on a sample value to a known population value.

  • Research Question : Are artists more likely to be left-handed than people found in the general population?
  • Response Variable : Classification of the student as either right-handed or left-handed

State Null and Alternative Hypotheses

  • Null Hypothesis : Students in the College of Arts and Architecture are no more likely to be left-handed than people in the general population (population percent of left-handed students in the College of Art and Architecture = 10% or p = .10).
  • Alternative Hypothesis : Students in the College of Arts and Architecture are more likely to be left-handed than people in the general population (population percent of left-handed students in the College of Arts and Architecture > 10% or p > .10). This is a one-sided alternative hypothesis.

Example 10.3: Hypotheses with One Sample of One Measurement Variable Section  

 two Diphenhydramine pills

A generic brand of the anti-histamine Diphenhydramine markets a capsule with a 50 milligram dose. The manufacturer is worried that the machine that fills the capsules has come out of calibration and is no longer creating capsules with the appropriate dosage.

  • Research Question : Does the data suggest that the population mean dosage of this brand is different than 50 mg?
  • Response Variable : dosage of the active ingredient found by a chemical assay.
  • Null Hypothesis : On the average, the dosage sold under this brand is 50 mg (population mean dosage = 50 mg).
  • Alternative Hypothesis : On the average, the dosage sold under this brand is not 50 mg (population mean dosage ≠ 50 mg). This is a two-sided alternative hypothesis.

Example 10.4: Hypotheses with Two Samples of One Categorical Variable Section  

vegetarian airline meal

Many people are starting to prefer vegetarian meals on a regular basis. Specifically, a researcher believes that females are more likely than males to eat vegetarian meals on a regular basis.

  • Research Question : Does the data suggest that females are more likely than males to eat vegetarian meals on a regular basis?
  • Response Variable : Classification of whether or not a person eats vegetarian meals on a regular basis
  • Explanatory (Grouping) Variable: Sex
  • Null Hypothesis : There is no sex effect regarding those who eat vegetarian meals on a regular basis (population percent of females who eat vegetarian meals on a regular basis = population percent of males who eat vegetarian meals on a regular basis or p females = p males ).
  • Alternative Hypothesis : Females are more likely than males to eat vegetarian meals on a regular basis (population percent of females who eat vegetarian meals on a regular basis > population percent of males who eat vegetarian meals on a regular basis or p females > p males ). This is a one-sided alternative hypothesis.

Example 10.5: Hypotheses with Two Samples of One Measurement Variable Section  

low carb meal

Obesity is a major health problem today. Research is starting to show that people may be able to lose more weight on a low carbohydrate diet than on a low fat diet.

  • Research Question : Does the data suggest that, on the average, people are able to lose more weight on a low carbohydrate diet than on a low fat diet?
  • Response Variable : Weight loss (pounds)
  • Explanatory (Grouping) Variable : Type of diet
  • Null Hypothesis : There is no difference in the mean amount of weight loss when comparing a low carbohydrate diet with a low fat diet (population mean weight loss on a low carbohydrate diet = population mean weight loss on a low fat diet).
  • Alternative Hypothesis : The mean weight loss should be greater for those on a low carbohydrate diet when compared with those on a low fat diet (population mean weight loss on a low carbohydrate diet > population mean weight loss on a low fat diet). This is a one-sided alternative hypothesis.

Example 10.6: Hypotheses about the relationship between Two Categorical Variables Section  

  • Research Question : Do the odds of having a stroke increase if you inhale second hand smoke ? A case-control study of non-smoking stroke patients and controls of the same age and occupation are asked if someone in their household smokes.
  • Variables : There are two different categorical variables (Stroke patient vs control and whether the subject lives in the same household as a smoker). Living with a smoker (or not) is the natural explanatory variable and having a stroke (or not) is the natural response variable in this situation.
  • Null Hypothesis : There is no relationship between whether or not a person has a stroke and whether or not a person lives with a smoker (odds ratio between stroke and second-hand smoke situation is = 1).
  • Alternative Hypothesis : There is a relationship between whether or not a person has a stroke and whether or not a person lives with a smoker (odds ratio between stroke and second-hand smoke situation is > 1). This is a one-tailed alternative.

This research question might also be addressed like example 11.4 by making the hypotheses about comparing the proportion of stroke patients that live with smokers to the proportion of controls that live with smokers.

Example 10.7: Hypotheses about the relationship between Two Measurement Variables Section  

  • Research Question : A financial analyst believes there might be a positive association between the change in a stock's price and the amount of the stock purchased by non-management employees the previous day (stock trading by management being under "insider-trading" regulatory restrictions).
  • Variables : Daily price change information (the response variable) and previous day stock purchases by non-management employees (explanatory variable). These are two different measurement variables.
  • Null Hypothesis : The correlation between the daily stock price change (\$) and the daily stock purchases by non-management employees (\$) = 0.
  • Alternative Hypothesis : The correlation between the daily stock price change (\$) and the daily stock purchases by non-management employees (\$) > 0. This is a one-sided alternative hypothesis.

Example 10.8: Hypotheses about comparing the relationship between Two Measurement Variables in Two Samples Section  

Calculation of a person's approximate tip for their meal

  • Research Question : Is there a linear relationship between the amount of the bill (\$) at a restaurant and the tip (\$) that was left. Is the strength of this association different for family restaurants than for fine dining restaurants?
  • Variables : There are two different measurement variables. The size of the tip would depend on the size of the bill so the amount of the bill would be the explanatory variable and the size of the tip would be the response variable.
  • Null Hypothesis : The correlation between the amount of the bill (\$) at a restaurant and the tip (\$) that was left is the same at family restaurants as it is at fine dining restaurants.
  • Alternative Hypothesis : The correlation between the amount of the bill (\$) at a restaurant and the tip (\$) that was left is the difference at family restaurants then it is at fine dining restaurants. This is a two-sided alternative hypothesis.

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15 Null Hypothesis Examples

15 Null Hypothesis Examples

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Dr. Chris Drew is the founder of the Helpful Professor. He holds a PhD in education and has published over 20 articles in scholarly journals. He is the former editor of the Journal of Learning Development in Higher Education. [Image Descriptor: Photo of Chris]

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null hypothesis example and definition, explained below

A null hypothesis is a general assertion or default position that there is no relationship or effect between two measured phenomena.

It’s a critical part of statistics, data analysis, and the scientific method . This concept forms the basis of testing statistical significance and allows researchers to be objective in their conclusions.

A null hypothesis helps to eliminate biases and ensures that the observed results are not due to chance. The rejection or failure to reject the null hypothesis helps in guiding the course of research.

chris

Null Hypothesis Definition

The null hypothesis, often denoted as H 0 , is the hypothesis in a statistical test which proposes no statistical significance exists in a set of observed data.

It hypothesizes that any kind of difference or importance you see in a data set is due to chance.

Null hypotheses are typically proposed to be negated or disproved by statistical tests, paving way for the acceptance of an alternate hypothesis.

Importantly, a null hypothesis cannot be proven true; it can only be supported or rejected with confidence.

Should evidence – via statistical analysis – contradict the null hypothesis, it is rejected in favor of an alternative hypothesis. In essence, the null hypothesis is a tool to challenge and disprove that there is no effect or relationship between variables.

Video Explanation

I like to show this video to my students which outlines a null hypothesis really clearly and engagingly, using real life studies by research students! The into explains it really well:

“There’s an idea in science called the null hypothesis and it works like this: when you’re setting out to prove a theory, your default answer should be “it’s not going to work” and you have to convince the world otherwise through clear results”

Here’s the full video:

Null Hypothesis Examples

  • Equality of Means: The null hypothesis posits that the average of group A does not differ from the average of group B. It suggests that any observed difference between the two group means is due to sampling or experimental error.
  • No Correlation: The null hypothesis states there is no correlation between the variable X and variable Y in the population. It means that any correlation seen in sample data occurred by chance.
  • Drug Effectiveness: The null hypothesis proposes that a new drug does not reduce the number of days to recover from a disease compared to a standard drug. Any observed difference is merely by chance and not due to the new drug.
  • Classroom Teaching Method: The null hypothesis states that a new teaching method does not result in improved test scores compared to the traditional teaching method. Any improvement in scores can be attributed to chance or other unrelated factors.
  • Smoking and Life Expectancy: The null hypothesis asserts that the average life expectancy of smokers is the same as that of non-smokers. Any perceived difference in life expectancy is due to random variation or other factors.
  • Brand Preference: The null hypothesis suggests that the proportion of consumers preferring Brand A is the same as those preferring Brand B. Any observed preference in the sample is due to random selection.
  • Vaccination Efficacy: The null hypothesis states that the efficacy of Vaccine A does not differ from that of Vaccine B. Any differences observed in a sample are due to chance or other confounding factors.
  • Diet and Weight Loss: The null hypothesis proposes that following a specific diet does not result in more weight loss than not following the diet. Any weight loss observed among dieters is considered random or influenced by other factors.
  • Exercise and Heart Rate: The null hypothesis states that regular exercise does not lower resting heart rate compared to no exercise. Any lower heart rates observed in exercisers could be due to chance or other unrelated factors.
  • Climate Change: The null hypothesis asserts that the average global temperature this decade is not higher than the previous decade. Any observed temperature increase can be attributed to random variation or unaccounted factors.
  • Gender Wage Gap: The null hypothesis posits that men and women earn the same average wage for the same job. Any observed wage disparity is due to chance or non-gender related factors.
  • Psychotherapy Effectiveness: The null hypothesis states that patients undergoing psychotherapy do not show more improvement than those not undergoing therapy. Any improvement in the
  • Energy Drink Consumption and Sleep: The null hypothesis proposes that consuming energy drinks does not affect the quantity of sleep. Any observed differences in sleep duration among energy drink consumers is due to random variation or other factors.
  • Organic Food and Health: The null hypothesis asserts that consuming organic food does not lead to better health outcomes compared to consuming non-organic food. Any health differences observed in consumers of organic food are considered random or attributed to other confounding factors.
  • Online Learning Effectiveness: The null hypothesis states that students learning online do not perform differently on exams than students learning in traditional classrooms. Any difference in performance can be attributed to chance or unrelated factors.

Null Hypothesis vs Alternative Hypothesis

An alternative hypothesis is the direct contrast to the null hypothesis. It posits that there is a statistically significant relationship or effect between the variables being observed.

If the null hypothesis is rejected based on the test data, the alternative hypothesis is accepted.

Importantly, while the null hypothesis is typically a statement of ‘no effect’ or ‘no difference,’ the alternative hypothesis states that there is an effect or difference.

Comprehension Checkpoint: How does the null hypothesis help to ensure that research is objective and unbiased?

A statement of no effect or no relationshipA statement that suggests there is an effect or relationship
H H or H
The average time to recover using Drug A is the same as with Drug BThe average time to recover using Drug A is less than with Drug B
No statistical significance between observed dataStatistical significance exists between observed data
The observed result is due to chanceThe observed result is due to the effect or relationship

Applications of the Null Hypothesis in Research

The null hypothesis plays a critical role in numerous research settings, promoting objectivity and ensuring findings aren’t due to random chance.

  • Clinical Trials: Null hypothesis is used extensively in medical and pharmaceutical research. For example, when testing a new drug’s effectiveness, the null hypothesis might state that the drug has no effect on the disease. If data contradicts this, the null hypothesis is rejected, suggesting the drug might be effective.
  • Business and Economics: Businesses use null hypotheses to make informed decisions. For instance, a company might use a null hypothesis to test if a new marketing strategy improves sales. If data suggests a significant increase in sales, the null hypothesis is rejected, and the new strategy may be implemented.
  • Psychological Research: Psychologists use null hypotheses to test theories about behavior. For instance, a null hypothesis might state there’s no link between stress and sleep quality. Rejecting this hypothesis based on collected data could help establish a correlation between the two variables.
  • Environmental Science: Null hypotheses are used to understand environmental changes. For instance, researchers might form a null hypothesis stating there is no significant difference in air quality before and after a policy change. If this hypothesis is rejected, it indicates the policy may have impacted air quality.
  • Education: Educators and researchers often use null hypotheses to improve teaching methods. For example, a null hypothesis might propose a new teaching technique doesn’t enhance student performance. If data contradicts this, the technique may be beneficial.

In all these areas, the null hypothesis helps minimize bias, enabling researchers to support their findings with statistically significant data. It forms the backbone of many scientific research methodologies , promoting a disciplined approach to uncovering new knowledge.

See More Hypothesis Examples Here

The null hypothesis is a cornerstone of statistical analysis and empirical research. It serves as a starting point for investigations, providing a baseline premise that the observed effects are due to chance. By understanding and applying the concept of the null hypothesis, researchers can test the validity of their assumptions, making their findings more robust and reliable. In essence, the null hypothesis ensures that the scientific exploration remains objective, systematic, and free from unintended bias.

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Chapter 13: Inferential Statistics

Understanding Null Hypothesis Testing

Learning Objectives

  • Explain the purpose of null hypothesis testing, including the role of sampling error.
  • Describe the basic logic of null hypothesis testing.
  • Describe the role of relationship strength and sample size in determining statistical significance and make reasonable judgments about statistical significance based on these two factors.

The Purpose of Null Hypothesis Testing

As we have seen, psychological research typically involves measuring one or more variables for a sample and computing descriptive statistics for that sample. In general, however, the researcher’s goal is not to draw conclusions about that sample but to draw conclusions about the population that the sample was selected from. Thus researchers must use sample statistics to draw conclusions about the corresponding values in the population. These corresponding values in the population are called  parameters . Imagine, for example, that a researcher measures the number of depressive symptoms exhibited by each of 50 clinically depressed adults and computes the mean number of symptoms. The researcher probably wants to use this sample statistic (the mean number of symptoms for the sample) to draw conclusions about the corresponding population parameter (the mean number of symptoms for clinically depressed adults).

Unfortunately, sample statistics are not perfect estimates of their corresponding population parameters. This is because there is a certain amount of random variability in any statistic from sample to sample. The mean number of depressive symptoms might be 8.73 in one sample of clinically depressed adults, 6.45 in a second sample, and 9.44 in a third—even though these samples are selected randomly from the same population. Similarly, the correlation (Pearson’s  r ) between two variables might be +.24 in one sample, −.04 in a second sample, and +.15 in a third—again, even though these samples are selected randomly from the same population. This random variability in a statistic from sample to sample is called  sampling error . (Note that the term error  here refers to random variability and does not imply that anyone has made a mistake. No one “commits a sampling error.”)

One implication of this is that when there is a statistical relationship in a sample, it is not always clear that there is a statistical relationship in the population. A small difference between two group means in a sample might indicate that there is a small difference between the two group means in the population. But it could also be that there is no difference between the means in the population and that the difference in the sample is just a matter of sampling error. Similarly, a Pearson’s  r  value of −.29 in a sample might mean that there is a negative relationship in the population. But it could also be that there is no relationship in the population and that the relationship in the sample is just a matter of sampling error.

In fact, any statistical relationship in a sample can be interpreted in two ways:

  • There is a relationship in the population, and the relationship in the sample reflects this.
  • There is no relationship in the population, and the relationship in the sample reflects only sampling error.

The purpose of null hypothesis testing is simply to help researchers decide between these two interpretations.

The Logic of Null Hypothesis Testing

Null hypothesis testing  is a formal approach to deciding between two interpretations of a statistical relationship in a sample. One interpretation is called the   null hypothesis  (often symbolized  H 0  and read as “H-naught”). This is the idea that there is no relationship in the population and that the relationship in the sample reflects only sampling error. Informally, the null hypothesis is that the sample relationship “occurred by chance.” The other interpretation is called the  alternative hypothesis  (often symbolized as  H 1 ). This is the idea that there is a relationship in the population and that the relationship in the sample reflects this relationship in the population.

Again, every statistical relationship in a sample can be interpreted in either of these two ways: It might have occurred by chance, or it might reflect a relationship in the population. So researchers need a way to decide between them. Although there are many specific null hypothesis testing techniques, they are all based on the same general logic. The steps are as follows:

  • Assume for the moment that the null hypothesis is true. There is no relationship between the variables in the population.
  • Determine how likely the sample relationship would be if the null hypothesis were true.
  • If the sample relationship would be extremely unlikely, then reject the null hypothesis  in favour of the alternative hypothesis. If it would not be extremely unlikely, then  retain the null hypothesis .

Following this logic, we can begin to understand why Mehl and his colleagues concluded that there is no difference in talkativeness between women and men in the population. In essence, they asked the following question: “If there were no difference in the population, how likely is it that we would find a small difference of  d  = 0.06 in our sample?” Their answer to this question was that this sample relationship would be fairly likely if the null hypothesis were true. Therefore, they retained the null hypothesis—concluding that there is no evidence of a sex difference in the population. We can also see why Kanner and his colleagues concluded that there is a correlation between hassles and symptoms in the population. They asked, “If the null hypothesis were true, how likely is it that we would find a strong correlation of +.60 in our sample?” Their answer to this question was that this sample relationship would be fairly unlikely if the null hypothesis were true. Therefore, they rejected the null hypothesis in favour of the alternative hypothesis—concluding that there is a positive correlation between these variables in the population.

A crucial step in null hypothesis testing is finding the likelihood of the sample result if the null hypothesis were true. This probability is called the  p value . A low  p  value means that the sample result would be unlikely if the null hypothesis were true and leads to the rejection of the null hypothesis. A high  p  value means that the sample result would be likely if the null hypothesis were true and leads to the retention of the null hypothesis. But how low must the  p  value be before the sample result is considered unlikely enough to reject the null hypothesis? In null hypothesis testing, this criterion is called  α (alpha)  and is almost always set to .05. If there is less than a 5% chance of a result as extreme as the sample result if the null hypothesis were true, then the null hypothesis is rejected. When this happens, the result is said to be  statistically significant . If there is greater than a 5% chance of a result as extreme as the sample result when the null hypothesis is true, then the null hypothesis is retained. This does not necessarily mean that the researcher accepts the null hypothesis as true—only that there is not currently enough evidence to conclude that it is true. Researchers often use the expression “fail to reject the null hypothesis” rather than “retain the null hypothesis,” but they never use the expression “accept the null hypothesis.”

The Misunderstood  p  Value

The  p  value is one of the most misunderstood quantities in psychological research (Cohen, 1994) [1] . Even professional researchers misinterpret it, and it is not unusual for such misinterpretations to appear in statistics textbooks!

The most common misinterpretation is that the  p  value is the probability that the null hypothesis is true—that the sample result occurred by chance. For example, a misguided researcher might say that because the  p  value is .02, there is only a 2% chance that the result is due to chance and a 98% chance that it reflects a real relationship in the population. But this is incorrect . The  p  value is really the probability of a result at least as extreme as the sample result  if  the null hypothesis  were  true. So a  p  value of .02 means that if the null hypothesis were true, a sample result this extreme would occur only 2% of the time.

You can avoid this misunderstanding by remembering that the  p  value is not the probability that any particular  hypothesis  is true or false. Instead, it is the probability of obtaining the  sample result  if the null hypothesis were true.

Role of Sample Size and Relationship Strength

Recall that null hypothesis testing involves answering the question, “If the null hypothesis were true, what is the probability of a sample result as extreme as this one?” In other words, “What is the  p  value?” It can be helpful to see that the answer to this question depends on just two considerations: the strength of the relationship and the size of the sample. Specifically, the stronger the sample relationship and the larger the sample, the less likely the result would be if the null hypothesis were true. That is, the lower the  p  value. This should make sense. Imagine a study in which a sample of 500 women is compared with a sample of 500 men in terms of some psychological characteristic, and Cohen’s  d  is a strong 0.50. If there were really no sex difference in the population, then a result this strong based on such a large sample should seem highly unlikely. Now imagine a similar study in which a sample of three women is compared with a sample of three men, and Cohen’s  d  is a weak 0.10. If there were no sex difference in the population, then a relationship this weak based on such a small sample should seem likely. And this is precisely why the null hypothesis would be rejected in the first example and retained in the second.

Of course, sometimes the result can be weak and the sample large, or the result can be strong and the sample small. In these cases, the two considerations trade off against each other so that a weak result can be statistically significant if the sample is large enough and a strong relationship can be statistically significant even if the sample is small. Table 13.1 shows roughly how relationship strength and sample size combine to determine whether a sample result is statistically significant. The columns of the table represent the three levels of relationship strength: weak, medium, and strong. The rows represent four sample sizes that can be considered small, medium, large, and extra large in the context of psychological research. Thus each cell in the table represents a combination of relationship strength and sample size. If a cell contains the word  Yes , then this combination would be statistically significant for both Cohen’s  d  and Pearson’s  r . If it contains the word  No , then it would not be statistically significant for either. There is one cell where the decision for  d  and  r  would be different and another where it might be different depending on some additional considerations, which are discussed in Section 13.2 “Some Basic Null Hypothesis Tests”

Table 13.1 How Relationship Strength and Sample Size Combine to Determine Whether a Result Is Statistically Significant
Sample Size Weak relationship Medium-strength relationship Strong relationship
Small (  = 20) No No  = Maybe

 = Yes

Medium (  = 50) No Yes Yes
Large (  = 100)  = Yes

 = No

Yes Yes
Extra large (  = 500) Yes Yes Yes

Although Table 13.1 provides only a rough guideline, it shows very clearly that weak relationships based on medium or small samples are never statistically significant and that strong relationships based on medium or larger samples are always statistically significant. If you keep this lesson in mind, you will often know whether a result is statistically significant based on the descriptive statistics alone. It is extremely useful to be able to develop this kind of intuitive judgment. One reason is that it allows you to develop expectations about how your formal null hypothesis tests are going to come out, which in turn allows you to detect problems in your analyses. For example, if your sample relationship is strong and your sample is medium, then you would expect to reject the null hypothesis. If for some reason your formal null hypothesis test indicates otherwise, then you need to double-check your computations and interpretations. A second reason is that the ability to make this kind of intuitive judgment is an indication that you understand the basic logic of this approach in addition to being able to do the computations.

Statistical Significance Versus Practical Significance

Table 13.1 illustrates another extremely important point. A statistically significant result is not necessarily a strong one. Even a very weak result can be statistically significant if it is based on a large enough sample. This is closely related to Janet Shibley Hyde’s argument about sex differences (Hyde, 2007) [2] . The differences between women and men in mathematical problem solving and leadership ability are statistically significant. But the word  significant  can cause people to interpret these differences as strong and important—perhaps even important enough to influence the college courses they take or even who they vote for. As we have seen, however, these statistically significant differences are actually quite weak—perhaps even “trivial.”

This is why it is important to distinguish between the  statistical  significance of a result and the  practical  significance of that result.  Practical significance refers to the importance or usefulness of the result in some real-world context. Many sex differences are statistically significant—and may even be interesting for purely scientific reasons—but they are not practically significant. In clinical practice, this same concept is often referred to as “clinical significance.” For example, a study on a new treatment for social phobia might show that it produces a statistically significant positive effect. Yet this effect still might not be strong enough to justify the time, effort, and other costs of putting it into practice—especially if easier and cheaper treatments that work almost as well already exist. Although statistically significant, this result would be said to lack practical or clinical significance.

Key Takeaways

  • Null hypothesis testing is a formal approach to deciding whether a statistical relationship in a sample reflects a real relationship in the population or is just due to chance.
  • The logic of null hypothesis testing involves assuming that the null hypothesis is true, finding how likely the sample result would be if this assumption were correct, and then making a decision. If the sample result would be unlikely if the null hypothesis were true, then it is rejected in favour of the alternative hypothesis. If it would not be unlikely, then the null hypothesis is retained.
  • The probability of obtaining the sample result if the null hypothesis were true (the  p  value) is based on two considerations: relationship strength and sample size. Reasonable judgments about whether a sample relationship is statistically significant can often be made by quickly considering these two factors.
  • Statistical significance is not the same as relationship strength or importance. Even weak relationships can be statistically significant if the sample size is large enough. It is important to consider relationship strength and the practical significance of a result in addition to its statistical significance.
  • Discussion: Imagine a study showing that people who eat more broccoli tend to be happier. Explain for someone who knows nothing about statistics why the researchers would conduct a null hypothesis test.
  • The correlation between two variables is  r  = −.78 based on a sample size of 137.
  • The mean score on a psychological characteristic for women is 25 ( SD  = 5) and the mean score for men is 24 ( SD  = 5). There were 12 women and 10 men in this study.
  • In a memory experiment, the mean number of items recalled by the 40 participants in Condition A was 0.50 standard deviations greater than the mean number recalled by the 40 participants in Condition B.
  • In another memory experiment, the mean scores for participants in Condition A and Condition B came out exactly the same!
  • A student finds a correlation of  r  = .04 between the number of units the students in his research methods class are taking and the students’ level of stress.

Long Descriptions

“Null Hypothesis” long description: A comic depicting a man and a woman talking in the foreground. In the background is a child working at a desk. The man says to the woman, “I can’t believe schools are still teaching kids about the null hypothesis. I remember reading a big study that conclusively disproved it years ago.” [Return to “Null Hypothesis”]

“Conditional Risk” long description: A comic depicting two hikers beside a tree during a thunderstorm. A bolt of lightning goes “crack” in the dark sky as thunder booms. One of the hikers says, “Whoa! We should get inside!” The other hiker says, “It’s okay! Lightning only kills about 45 Americans a year, so the chances of dying are only one in 7,000,000. Let’s go on!” The comic’s caption says, “The annual death rate among people who know that statistic is one in six.” [Return to “Conditional Risk”]

Media Attributions

  • Null Hypothesis by XKCD  CC BY-NC (Attribution NonCommercial)
  • Conditional Risk by XKCD  CC BY-NC (Attribution NonCommercial)
  • Cohen, J. (1994). The world is round: p < .05. American Psychologist, 49 , 997–1003. ↵
  • Hyde, J. S. (2007). New directions in the study of gender similarities and differences. Current Directions in Psychological Science, 16 , 259–263. ↵

Values in a population that correspond to variables measured in a study.

The random variability in a statistic from sample to sample.

A formal approach to deciding between two interpretations of a statistical relationship in a sample.

The idea that there is no relationship in the population and that the relationship in the sample reflects only sampling error.

The idea that there is a relationship in the population and that the relationship in the sample reflects this relationship in the population.

When the relationship found in the sample would be extremely unlikely, the idea that the relationship occurred “by chance” is rejected.

When the relationship found in the sample is likely to have occurred by chance, the null hypothesis is not rejected.

The probability that, if the null hypothesis were true, the result found in the sample would occur.

How low the p value must be before the sample result is considered unlikely in null hypothesis testing.

When there is less than a 5% chance of a result as extreme as the sample result occurring and the null hypothesis is rejected.

Research Methods in Psychology - 2nd Canadian Edition Copyright © 2015 by Paul C. Price, Rajiv Jhangiani, & I-Chant A. Chiang is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License , except where otherwise noted.

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Null and Alternative Hypothesis: Research Guidelines

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When undertaking a qualitative or quantitative research project, researchers must first formulate a research question, from which they develop their theories. By definition, an assumption is a prediction that a researcher makes about an actual research question and can either be affirmative or negative. In this case, writing a research question has three main components: variables (independent and dependent), a population sample, and the relation between these variables. To find null and alternative hypotheses, scholars identify a specific research question, determine the variables involved, and state H0 as no effect or difference and H1 or Ha as a significant effect or difference. When the prediction contradicts the research question, it is referred to as a null assumption. In short, an initial theory is a statement that implies there is no relationship between independent and dependent variables. Hence, researchers need to learn how to write a good null and alternative hypothesis to present quality studies.

General Aspects

Students with qualitative or quantitative research assignments must learn how to formulate and write good research questions and proposition statements. In essence, hypothesis testing is a statistical method used to determine if there is enough evidence to reject an initial theory and support an alternative assumption based on sample data. By definition, a research proposition is an assumption or prediction that a scholar makes before undertaking an experimental investigation. Basically, academic standards require such a prediction to be a precise and testable statement, meaning that researchers must prove or disapprove of it in the course of their assignment and provide alternatives if possible. In this case, the main components of a typical research assumption are variables (independent and dependent), a population sample, and the relation between these variables. To formulate a null hypothesis (H0) in quantitative research, researchers state there is no effect or difference between variables (e.g., µ1 = µ2), and, for an alternative hypothesis (H1 or Ha), they posit there is a significant effect or difference (e.g., µ1 ≠ µ2). Therefore, a research proposition is a prediction that scholars write about the relationship between two or more variables. In turn, a standard research inquiry is a particular process that seeks to answer a specific research question and, in the process, test a particular theory by confirming or disapproving it.

How to write a null and alternative hypothesis

Types of Hypotheses

There are several types of hypotheses, including null, alternative, directional, and non-directional assumptions. Basically, a directional hypothesis is a prediction of how an independent variable affects a dependent variable. In contrast, a non-directional hypothesis predicts that an independent variable influences a dependent variable but does not specify how. Regardless of the type, all propositions are about predicting the relationship between independent and dependent variables. To write H0 (null assumption) and H1 or Ha (alternative prediction), researchers clearly state H0 as a central assumption of no effect or no difference (e.g., µ1 = µ2) and H1 or Ha as a secondary assumption of a significant effect or difference (e.g., µ1 ≠ µ2).

What Is a Null Hypothesis (H0) and Its Purpose

According to its definition, a null hypothesis is a foundational statement in statistical testing that posits there is no significant effect, relationship, or difference between groups or variables within a given study. In simple words, a null hypothesis, usually symbolized as “H0,” is a statement that contradicts an actual research theory (Watt & Collins, 2019). The main purpose of writing a null hypothesis is to provide a basis for comparison, allowing researchers to determine whether there is sufficient evidence to reject this assumption in favor of an alternative theory, which suggests a real effect or relationship. As such, it is a negative statement, indicating that there is no relationship or connection between independent and dependent variables (Harrison et al., 2020). By starting with a null proposition, researchers can also employ various statistical tests to evaluate an entire data, ensuring the objectivity of findings and minimizing their bias. The process helps to ensure the validity of scientific research, minimizing the likelihood of drawing incorrect conclusions from the data collected. Moreover, by testing an initial theory, researchers can determine whether the inquiry results are due to the chance or the effect of manipulating a dependent variable (McNulty, 2022). In most instances, a null assumption corresponds with an alternative theory, a positive statement that covers a relationship that exists between independent and dependent variables. Finally, it is highly recommended that researchers should write an alternative assumption first before a null proposition.

What Is an Alternative Hypothesis (H1 or Ha) and Its Purpose

According to its definition and opposite to a null assumption, an alternative hypothesis in research is another statement in statistical testing that suggests there is a significant effect, relationship, or difference between groups or variables in a given study. Basically, this statement contrasts with what a null theory posits, which asserts that no such effect or relationship exists (Baker, 2021). The main purpose of writing an alternative hypothesis is to guide researchers in testing and validating new theories or effects and determine whether the observed data can provide evidence against a null proposition. The process involves comparing observed results to what would be expected under a null assumption. When statistical tests provide enough evidence to reject an initial postulation, an alternative theory becomes true, indicating that the observed effect or relationship is likely real and not due to random variation (Jawlik, 2016). By framing their research around an alternative hypothesis, scientists can focus their investigations on discovering meaningful effects and relationships, thereby advancing knowledge and understanding in their study fields. Hence, writing good null and alternative hypotheses is important because they provide a structured framework for statistical testing, allowing researchers to objectively evaluate evidence and draw conclusions about the presence of significant effects or relationships in an entire data.

Null vs. Alternative Hypothesis Formats

AspectNull Assumption (H0)Alternative Prediction (H1 or Ha)
MeaningAssumes no effect or relationshipAssumes a significant effect or relationship
PurposeServes as a default or baseline assumptionProvides a specific direction for research
ClaimNo difference or effect existsA difference or effect exists
Statistical TestingBasis for comparisonSought to be supported by rejecting H0
Example (Means)µ1 = µ2µ1 ≠ µ2, µ1 > µ2, or µ1 < µ2
Example (Proportions)p1 = p2p1 ≠ p2, p1 > p2, or p1 < p2
Role in ResearchUsed to test for significance of resultsIndicates a presence of an effect if H0 is rejected
Decision MakingRetain H0 if there is insufficient evidence to rejectAccept H1 or Ha as an alternative option if there is sufficient evidence to reject H0
Result InterpretationObserved effect is due to chanceObserved effect is real and not due to chance
Scientific InquiryMaintains objectivity and prevents false positivesProvides alternative discovery and theory testing

Steps on How to Write a Good Null and Alternative Hypothesis

  • Identify a Specific Research Question: Start with clearly defining a particular problem or phenomenon you want to study.
  • Determine Key Variables: Identify independent and dependent variables involved in your study.
  • State a Specific Null Hypothesis (H0): Formulate a concrete statement that suggests no effect, no difference, or no relationship between your variables. This is usually a statement of equality (e.g., µ1 = µ2).
  • State a Clear Alternative Hypothesis (H1 or Ha): Formulate another statement that suggests a significant effect, difference, or relationship between your variables. This is usually a statement of inequality (e.g., µ1 ≠ µ2, µ1 > µ2, or µ1 < µ2).
  • Means: H0: µ1 = µ2 vs. H1: µ1 ≠ µ2
  • Proportions: H0: p1 = p2 vs. H1: p1 ≠ p2
  • One-tailed test: If you are testing for a specific direction of effect (e.g., H1: µ1 > µ2).
  • Two-tailed test: If you are testing for any difference, regardless of direction (e.g., H1: µ1 ≠ µ2).
  • Consult Literature: Review existing research to see how similar or alternative theories have been formulated. This can provide guidance and ensure your expectations are aligned with standard practices in your field.
  • Write in Simple Terms: Ensure both null and alternative theories are stated clearly and concisely, making them easy to understand.
  • Review and Refine: Double-check your propositions for clarity and correctness. Make sure they are mutually exclusive and collectively exhaustive, covering all possible outcomes.
  • Seek Feedback: Discuss your approaches with peers or advisors to ensure they are logical, relevant, and testable. Adjust as necessary based on their input.

Note: A null hypothesis is a specific statement assuming no effect or difference, while other hypotheses refer to general statements that include writing null and alternative hypotheses and proposing possible outcomes to be tested.

Written Examples of Research Questions With H0 and H1 Hypotheses

Before developing any study proposition, a researcher must formulate a specific research question. In this case, a research hypothesis is a broad, testable statement about the expected relationship between variables, while a statistical hypothesis specifically refers to writing null and alternative hypotheses used in statistical testing to validate or refute an initial study assumption (O’Donnell et al., 2023). Then, the next step is to transform this study question into a negative statement that claims the lack of a relationship between independent and dependent variables. Alternatively, researchers can change the question into a positive statement that includes a relationship that exists between the variables. In turn, this latter statement becomes an alternative hypothesis and is symbolized as H1 or Ha. Hence, some of the examples of research questions and hull and alternative hypotheses are as follows:

Research Question (RQ) 1: Do physical exercises help individuals to age gracefully?

  • A Null Hypothesis (H0): Physical exercises are not a guarantee for graceful old age.
  • An Alternative Hypothesis (H1): Engaging in physical exercises enables individuals to remain healthy and active into old age.

RQ 2: What are the implications of therapeutic interventions in the fight against substance abuse?

  • H0: Therapeutic interventions are of no help in the fight against substance abuse.
  • H1: Exposing individuals with substance abuse disorders to therapeutic interventions helps to control and even stop their addictions.

RQ 3: How do sexual orientation and gender identity affect the experiences of late adolescents in foster care?

  • H0: Sexual orientation and gender identity have no effects on the experiences of late adolescents in foster care.
  • H1: The reality of stereotypes in society makes sexual orientation and gender identity factors complicate the experiences of late adolescents in foster care.

RQ 4: Does income inequality contribute to crime in high-density urban areas?

  • H0: There is no correlation between income inequality and incidences of crime in high-density urban areas.
  • H1: The high crime rates in high-density urban areas are due to the incidence of income inequality in those areas.

RQ 5: Does placement in foster care impact individuals’ mental health?

  • H0: There is no correlation between being in foster care and having mental health problems.
  • H1: Individuals placed in foster care experience anxiety and depression at one point in their life.

RQ 6: Do assistive devices and technologies lessen the mobility challenges of older adults with a stroke?

  • H0: Assistive devices and technologies do not provide any assistance to the mobility of older adults diagnosed with a stroke.
  • H1: Assistive devices and technologies enhance the mobility of older adults diagnosed with a stroke.

RQ 7: Does race identity undermine classroom participation?

  • H0: There is no correlation between racial identity and the ability to participate in classroom learning.
  • H1: Students from racial minorities are not as active as white students in classroom participation.

RQ 8: Do high school grades determine future success?

  • H0: There is no correlation between how one performs in high school and their success level in life.
  • H1: Attaining high grades in high school positions one for greater success in the future personal and professional lives.

RQ 9: Does critical thinking predict academic achievement?

  • H0: There is no correlation between critical thinking and academic achievement.
  • H1: Being a critical thinker is a pathway to academic success.

RQ 10: What benefits does group therapy provide to victims of domestic violence?

  • H0: Group therapy does not help victims of domestic violence because individuals prefer to hide rather than expose their shame.
  • H1: Group therapy provides domestic violence victims with a platform to share their hurt and connect with others with similar experiences.

Symbols and Signs in Writing

AspectNull Theory (H0)Alternative Proposition (H1 or Ha)
SymbolsH0H1 or Ha
Equality Sign=
Greater Than SignNot used
Less Than SignNot used
Meansµ1 = µ2: No difference in population meansµ1 ≠ µ2: Population means are different
Proportionsp1 = p2: No difference in population proportionsp1 ≠ p2: Population proportions are different
p1 > p2: Proportion of population 1 is greater than proportion of population 2
p1 < p2: Proportion of population 1 is less than proportion of population 2
Testing ApproachServes as a default assumption; tested to be retained or rejectedProvides an alternative claim to be tested for evidence
Outcome if AcceptedIndicates that the observed data is due to chance in a null theoryIndicates that the observed data shows a true effect or relationship as an alternative to a null statement

Common Mistakes

  • Ambiguity in Theories: Writing vague or unclear null and alternative assumptions.
  • Directional vs. Non-Directional Confusion: Confusing one-tailed (directional) and two-tailed (non-directional) claims.
  • Using Sample Statistics: Stating initial and alternative propositions in terms of sample statistics instead of population parameters.
  • Overlapping Assumptions: Creating null and alternative statements that are not mutually exclusive.
  • Testing Multiple Variables: Including multiple variables or conditions in a single theory.
  • Misinterpreting a Null Proposition: Assuming an initial statement is what you want to prove.
  • Incorrect Symbols and Signs: Using incorrect or inconsistent symbols and signs for writing null and alternative propositions.
  • Ignoring Context: Writing initial and alternative theories that are not relevant to an assigned research question or context.
  • Not Testable Hypotheses: Formulating null and alternative statements that are not testable with the available data or methods.
  • Confusing Null and Alternative Hypotheses: Swapping the roles of null and alternative assumptions.

The formulation of research questions in qualitative and quantitative assignments helps students to develop a specific theory for their experiments. In this case, learning how to write a good null and alternative hypothesis helps students and researchers to make their research relevant. Basically, the difference between a null and alternative hypothesis is that the former contradicts an entire research question, while the latter affirms it. In short, an initial proposition is a negative statement relative to a particular research question, and an alternative theory is a positive assumption. Moreover, it is important to note that developing a null hypothesis at the beginning of the assignment is for prediction purposes. As such, the research work must answer a specific research question and confirm or disapprove of an initial proposition. Hence, some of the tips that students and researchers need to know when developing any theory include:

  • Formulate a research question that specifies the relationship between an independent variable and a dependent variable.
  • Develop an alternative assumption that says a relationship exists between the variables.
  • Develop a null proposition that says a relationship does not exist between the variables.
  • Conduct an experiment to answer a research question under analysis, which allows the confirmation of a disapproval of a null theory or considering alternative options.

Baker, L. (2021). Hypothesis testing: How to choose the correct test (Getting started with statistics) . Chi-Squared Innovations.

Harrison, A. J., McErlain-Naylor, S. A., Bradshaw, E. J., Dai, B., Nunome, H., Hughes, G. T. G., Kong, P. W., Vanwanseele, B., Vilas-Boas, J. P., & Fong, D. T. (2020). Recommendations for statistical analysis involving null hypothesis significance testing. Sports Biomechanics , 19 (5), 561–568. https://doi.org/10.1080/14763141.2020.1782555

Jawlik, A. (2016). Statistics from A to Z: Confusing concepts clarified . John Wiley & Sons, Inc.

McNulty, R. (2022). A logical analysis of null hypothesis significance testing using popular terminology. BMC Medical Research Methodology , 22 (1), 1–9. https://doi.org/10.1186/s12874-022-01696-5

O’Donnell, C. T., Fielding-Singh, V., & Vanneman, M. W. (2023). The art of the null hypothesis — Considerations for study design and scientific reporting. Journal of Cardiothoracic and Vascular Anesthesia , 37 (6), 867–869. https://doi.org/10.1053/j.jvca.2023.02.026

Watt, R., & Collins, E. (2019). Null hypothesis testing . SAGE Publications Ltd.

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Neag School of Education

Educational Research Basics by Del Siegle

Null and alternative hypotheses.

Converting research questions to hypothesis is a simple task. Take the questions and make it a positive statement that says a relationship exists (correlation studies) or a difference exists between the groups (experiment study) and you have the alternative hypothesis. Write the statement such that a relationship does not exist or a difference does not exist and you have the null hypothesis. You can reverse the process if you have a hypothesis and wish to write a research question.

When you are comparing two groups, the groups are the independent variable. When you are testing whether something affects something else, the cause is the independent variable. The independent variable is the one you manipulate.

Teachers given higher pay will have more positive attitudes toward children than teachers given lower pay. The first step is to ask yourself “Are there two or more groups being compared?” The answer is “Yes.” What are the groups? Teachers who are given higher pay and teachers who are given lower pay. The independent variable is teacher pay. The dependent variable (the outcome) is attitude towards school.

You could also approach is another way. “Is something causing something else?” The answer is “Yes.”  What is causing what? Teacher pay is causing attitude towards school. Therefore, teacher pay is the independent variable (cause) and attitude towards school is the dependent variable (outcome).

By tradition, we try to disprove (reject) the null hypothesis. We can never prove a null hypothesis, because it is impossible to prove something does not exist. We can disprove something does not exist by finding an example of it. Therefore, in research we try to disprove the null hypothesis. When we do find that a relationship (or difference) exists then we reject the null and accept the alternative. If we do not find that a relationship (or difference) exists, we fail to reject the null hypothesis (and go with it). We never say we accept the null hypothesis because it is never possible to prove something does not exist. That is why we say that we failed to reject the null hypothesis, rather than we accepted it.

Del Siegle, Ph.D. Neag School of Education – University of Connecticut [email protected] www.delsiegle.com

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Null hypothesis significance testing: a short tutorial

Cyril pernet.

1 Centre for Clinical Brain Sciences (CCBS), Neuroimaging Sciences, The University of Edinburgh, Edinburgh, UK

Version Changes

Revised. amendments from version 2.

This v3 includes minor changes that reflect the 3rd reviewers' comments - in particular the theoretical vs. practical difference between Fisher and Neyman-Pearson. Additional information and reference is also included regarding the interpretation of p-value for low powered studies.

Peer Review Summary

Review dateReviewer name(s)Version reviewedReview status
Dorothy Vera Margaret Bishop Approved with Reservations
Stephen J. Senn Approved
Stephen J. Senn Approved with Reservations
Marcel ALM van Assen Not Approved
Daniel Lakens Not Approved

Although thoroughly criticized, null hypothesis significance testing (NHST) remains the statistical method of choice used to provide evidence for an effect, in biological, biomedical and social sciences. In this short tutorial, I first summarize the concepts behind the method, distinguishing test of significance (Fisher) and test of acceptance (Newman-Pearson) and point to common interpretation errors regarding the p-value. I then present the related concepts of confidence intervals and again point to common interpretation errors. Finally, I discuss what should be reported in which context. The goal is to clarify concepts to avoid interpretation errors and propose reporting practices.

The Null Hypothesis Significance Testing framework

NHST is a method of statistical inference by which an experimental factor is tested against a hypothesis of no effect or no relationship based on a given observation. The method is a combination of the concepts of significance testing developed by Fisher in 1925 and of acceptance based on critical rejection regions developed by Neyman & Pearson in 1928 . In the following I am first presenting each approach, highlighting the key differences and common misconceptions that result from their combination into the NHST framework (for a more mathematical comparison, along with the Bayesian method, see Christensen, 2005 ). I next present the related concept of confidence intervals. I finish by discussing practical aspects in using NHST and reporting practice.

Fisher, significance testing, and the p-value

The method developed by ( Fisher, 1934 ; Fisher, 1955 ; Fisher, 1959 ) allows to compute the probability of observing a result at least as extreme as a test statistic (e.g. t value), assuming the null hypothesis of no effect is true. This probability or p-value reflects (1) the conditional probability of achieving the observed outcome or larger: p(Obs≥t|H0), and (2) is therefore a cumulative probability rather than a point estimate. It is equal to the area under the null probability distribution curve from the observed test statistic to the tail of the null distribution ( Turkheimer et al. , 2004 ). The approach proposed is of ‘proof by contradiction’ ( Christensen, 2005 ), we pose the null model and test if data conform to it.

In practice, it is recommended to set a level of significance (a theoretical p-value) that acts as a reference point to identify significant results, that is to identify results that differ from the null-hypothesis of no effect. Fisher recommended using p=0.05 to judge whether an effect is significant or not as it is roughly two standard deviations away from the mean for the normal distribution ( Fisher, 1934 page 45: ‘The value for which p=.05, or 1 in 20, is 1.96 or nearly 2; it is convenient to take this point as a limit in judging whether a deviation is to be considered significant or not’). A key aspect of Fishers’ theory is that only the null-hypothesis is tested, and therefore p-values are meant to be used in a graded manner to decide whether the evidence is worth additional investigation and/or replication ( Fisher, 1971 page 13: ‘it is open to the experimenter to be more or less exacting in respect of the smallness of the probability he would require […]’ and ‘no isolated experiment, however significant in itself, can suffice for the experimental demonstration of any natural phenomenon’). How small the level of significance is, is thus left to researchers.

What is not a p-value? Common mistakes

The p-value is not an indication of the strength or magnitude of an effect . Any interpretation of the p-value in relation to the effect under study (strength, reliability, probability) is wrong, since p-values are conditioned on H0. In addition, while p-values are randomly distributed (if all the assumptions of the test are met) when there is no effect, their distribution depends of both the population effect size and the number of participants, making impossible to infer strength of effect from them.

Similarly, 1-p is not the probability to replicate an effect . Often, a small value of p is considered to mean a strong likelihood of getting the same results on another try, but again this cannot be obtained because the p-value is not informative on the effect itself ( Miller, 2009 ). Because the p-value depends on the number of subjects, it can only be used in high powered studies to interpret results. In low powered studies (typically small number of subjects), the p-value has a large variance across repeated samples, making it unreliable to estimate replication ( Halsey et al. , 2015 ).

A (small) p-value is not an indication favouring a given hypothesis . Because a low p-value only indicates a misfit of the null hypothesis to the data, it cannot be taken as evidence in favour of a specific alternative hypothesis more than any other possible alternatives such as measurement error and selection bias ( Gelman, 2013 ). Some authors have even argued that the more (a priori) implausible the alternative hypothesis, the greater the chance that a finding is a false alarm ( Krzywinski & Altman, 2013 ; Nuzzo, 2014 ).

The p-value is not the probability of the null hypothesis p(H0), of being true, ( Krzywinski & Altman, 2013 ). This common misconception arises from a confusion between the probability of an observation given the null p(Obs≥t|H0) and the probability of the null given an observation p(H0|Obs≥t) that is then taken as an indication for p(H0) (see Nickerson, 2000 ).

Neyman-Pearson, hypothesis testing, and the α-value

Neyman & Pearson (1933) proposed a framework of statistical inference for applied decision making and quality control. In such framework, two hypotheses are proposed: the null hypothesis of no effect and the alternative hypothesis of an effect, along with a control of the long run probabilities of making errors. The first key concept in this approach, is the establishment of an alternative hypothesis along with an a priori effect size. This differs markedly from Fisher who proposed a general approach for scientific inference conditioned on the null hypothesis only. The second key concept is the control of error rates . Neyman & Pearson (1928) introduced the notion of critical intervals, therefore dichotomizing the space of possible observations into correct vs. incorrect zones. This dichotomization allows distinguishing correct results (rejecting H0 when there is an effect and not rejecting H0 when there is no effect) from errors (rejecting H0 when there is no effect, the type I error, and not rejecting H0 when there is an effect, the type II error). In this context, alpha is the probability of committing a Type I error in the long run. Alternatively, Beta is the probability of committing a Type II error in the long run.

The (theoretical) difference in terms of hypothesis testing between Fisher and Neyman-Pearson is illustrated on Figure 1 . In the 1 st case, we choose a level of significance for observed data of 5%, and compute the p-value. If the p-value is below the level of significance, it is used to reject H0. In the 2 nd case, we set a critical interval based on the a priori effect size and error rates. If an observed statistic value is below and above the critical values (the bounds of the confidence region), it is deemed significantly different from H0. In the NHST framework, the level of significance is (in practice) assimilated to the alpha level, which appears as a simple decision rule: if the p-value is less or equal to alpha, the null is rejected. It is however a common mistake to assimilate these two concepts. The level of significance set for a given sample is not the same as the frequency of acceptance alpha found on repeated sampling because alpha (a point estimate) is meant to reflect the long run probability whilst the p-value (a cumulative estimate) reflects the current probability ( Fisher, 1955 ; Hubbard & Bayarri, 2003 ).

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The figure was prepared with G-power for a one-sided one-sample t-test, with a sample size of 32 subjects, an effect size of 0.45, and error rates alpha=0.049 and beta=0.80. In Fisher’s procedure, only the nil-hypothesis is posed, and the observed p-value is compared to an a priori level of significance. If the observed p-value is below this level (here p=0.05), one rejects H0. In Neyman-Pearson’s procedure, the null and alternative hypotheses are specified along with an a priori level of acceptance. If the observed statistical value is outside the critical region (here [-∞ +1.69]), one rejects H0.

Acceptance or rejection of H0?

The acceptance level α can also be viewed as the maximum probability that a test statistic falls into the rejection region when the null hypothesis is true ( Johnson, 2013 ). Therefore, one can only reject the null hypothesis if the test statistics falls into the critical region(s), or fail to reject this hypothesis. In the latter case, all we can say is that no significant effect was observed, but one cannot conclude that the null hypothesis is true. This is another common mistake in using NHST: there is a profound difference between accepting the null hypothesis and simply failing to reject it ( Killeen, 2005 ). By failing to reject, we simply continue to assume that H0 is true, which implies that one cannot argue against a theory from a non-significant result (absence of evidence is not evidence of absence). To accept the null hypothesis, tests of equivalence ( Walker & Nowacki, 2011 ) or Bayesian approaches ( Dienes, 2014 ; Kruschke, 2011 ) must be used.

Confidence intervals

Confidence intervals (CI) are builds that fail to cover the true value at a rate of alpha, the Type I error rate ( Morey & Rouder, 2011 ) and therefore indicate if observed values can be rejected by a (two tailed) test with a given alpha. CI have been advocated as alternatives to p-values because (i) they allow judging the statistical significance and (ii) provide estimates of effect size. Assuming the CI (a)symmetry and width are correct (but see Wilcox, 2012 ), they also give some indication about the likelihood that a similar value can be observed in future studies. For future studies of the same sample size, 95% CI give about 83% chance of replication success ( Cumming & Maillardet, 2006 ). If sample sizes however differ between studies, CI do not however warranty any a priori coverage.

Although CI provide more information, they are not less subject to interpretation errors (see Savalei & Dunn, 2015 for a review). The most common mistake is to interpret CI as the probability that a parameter (e.g. the population mean) will fall in that interval X% of the time. The correct interpretation is that, for repeated measurements with the same sample sizes, taken from the same population, X% of times the CI obtained will contain the true parameter value ( Tan & Tan, 2010 ). The alpha value has the same interpretation as testing against H0, i.e. we accept that 1-alpha CI are wrong in alpha percent of the times in the long run. This implies that CI do not allow to make strong statements about the parameter of interest (e.g. the mean difference) or about H1 ( Hoekstra et al. , 2014 ). To make a statement about the probability of a parameter of interest (e.g. the probability of the mean), Bayesian intervals must be used.

The (correct) use of NHST

NHST has always been criticized, and yet is still used every day in scientific reports ( Nickerson, 2000 ). One question to ask oneself is what is the goal of a scientific experiment at hand? If the goal is to establish a discrepancy with the null hypothesis and/or establish a pattern of order, because both requires ruling out equivalence, then NHST is a good tool ( Frick, 1996 ; Walker & Nowacki, 2011 ). If the goal is to test the presence of an effect and/or establish some quantitative values related to an effect, then NHST is not the method of choice since testing is conditioned on H0.

While a Bayesian analysis is suited to estimate that the probability that a hypothesis is correct, like NHST, it does not prove a theory on itself, but adds its plausibility ( Lindley, 2000 ). No matter what testing procedure is used and how strong results are, ( Fisher, 1959 p13) reminds us that ‘ […] no isolated experiment, however significant in itself, can suffice for the experimental demonstration of any natural phenomenon'. Similarly, the recent statement of the American Statistical Association ( Wasserstein & Lazar, 2016 ) makes it clear that conclusions should be based on the researchers understanding of the problem in context, along with all summary data and tests, and that no single value (being p-values, Bayesian factor or else) can be used support or invalidate a theory.

What to report and how?

Considering that quantitative reports will always have more information content than binary (significant or not) reports, we can always argue that raw and/or normalized effect size, confidence intervals, or Bayes factor must be reported. Reporting everything can however hinder the communication of the main result(s), and we should aim at giving only the information needed, at least in the core of a manuscript. Here I propose to adopt optimal reporting in the result section to keep the message clear, but have detailed supplementary material. When the hypothesis is about the presence/absence or order of an effect, and providing that a study has sufficient power, NHST is appropriate and it is sufficient to report in the text the actual p-value since it conveys the information needed to rule out equivalence. When the hypothesis and/or the discussion involve some quantitative value, and because p-values do not inform on the effect, it is essential to report on effect sizes ( Lakens, 2013 ), preferably accompanied with confidence or credible intervals. The reasoning is simply that one cannot predict and/or discuss quantities without accounting for variability. For the reader to understand and fully appreciate the results, nothing else is needed.

Because science progress is obtained by cumulating evidence ( Rosenthal, 1991 ), scientists should also consider the secondary use of the data. With today’s electronic articles, there are no reasons for not including all of derived data: mean, standard deviations, effect size, CI, Bayes factor should always be included as supplementary tables (or even better also share raw data). It is also essential to report the context in which tests were performed – that is to report all of the tests performed (all t, F, p values) because of the increase type one error rate due to selective reporting (multiple comparisons and p-hacking problems - Ioannidis, 2005 ). Providing all of this information allows (i) other researchers to directly and effectively compare their results in quantitative terms (replication of effects beyond significance, Open Science Collaboration, 2015 ), (ii) to compute power to future studies ( Lakens & Evers, 2014 ), and (iii) to aggregate results for meta-analyses whilst minimizing publication bias ( van Assen et al. , 2014 ).

[version 3; referees: 1 approved

Funding Statement

The author(s) declared that no grants were involved in supporting this work.

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Referee response for version 3

Dorothy vera margaret bishop.

1 Department of Experimental Psychology, University of Oxford, Oxford, UK

I can see from the history of this paper that the author has already been very responsive to reviewer comments, and that the process of revising has now been quite protracted.

That makes me reluctant to suggest much more, but I do see potential here for making the paper more impactful. So my overall view is that, once a few typos are fixed (see below), this could be published as is, but I think there is an issue with the potential readership and that further revision could overcome this.

I suspect my take on this is rather different from other reviewers, as I do not regard myself as a statistics expert, though I am on the more quantitative end of the continuum of psychologists and I try to keep up to date. I think I am quite close to the target readership , insofar as I am someone who was taught about statistics ages ago and uses stats a lot, but never got adequate training in the kinds of topic covered by this paper. The fact that I am aware of controversies around the interpretation of confidence intervals etc is simply because I follow some discussions of this on social media. I am therefore very interested to have a clear account of these issues.

This paper contains helpful information for someone in this position, but it is not always clear, and I felt the relevance of some of the content was uncertain. So here are some recommendations:

  • As one previous reviewer noted, it’s questionable that there is a need for a tutorial introduction, and the limited length of this article does not lend itself to a full explanation. So it might be better to just focus on explaining as clearly as possible the problems people have had in interpreting key concepts. I think a title that made it clear this was the content would be more appealing than the current one.
  • P 3, col 1, para 3, last sentence. Although statisticians always emphasise the arbitrary nature of p < .05, we all know that in practice authors who use other values are likely to have their analyses queried. I wondered whether it would be useful here to note that in some disciplines different cutoffs are traditional, e.g. particle physics. Or you could cite David Colquhoun’s paper in which he recommends using p < .001 ( http://rsos.royalsocietypublishing.org/content/1/3/140216) - just to be clear that the traditional p < .05 has been challenged.

What I can’t work out is how you would explain the alpha from Neyman-Pearson in the same way (though I can see from Figure 1 that with N-P you could test an alternative hypothesis, such as the idea that the coin would be heads 75% of the time).

‘By failing to reject, we simply continue to assume that H0 is true, which implies that one cannot….’ have ‘In failing to reject, we do not assume that H0 is true; one cannot argue against a theory from a non-significant result.’

I felt most readers would be interested to read about tests of equivalence and Bayesian approaches, but many would be unfamiliar with these and might like to see an example of how they work in practice – if space permitted.

  • Confidence intervals: I simply could not understand the first sentence – I wondered what was meant by ‘builds’ here. I understand about difficulties in comparing CI across studies when sample sizes differ, but I did not find the last sentence on p 4 easy to understand.
  • P 5: The sentence starting: ‘The alpha value has the same interpretation’ was also hard to understand, especially the term ‘1-alpha CI’. Here too I felt some concrete illustration might be helpful to the reader. And again, I also found the reference to Bayesian intervals tantalising – I think many readers won’t know how to compute these and something like a figure comparing a traditional CI with a Bayesian interval and giving a source for those who want to read on would be very helpful. The reference to ‘credible intervals’ in the penultimate paragraph is very unclear and needs a supporting reference – most readers will not be familiar with this concept.

P 3, col 1, para 2, line 2; “allows us to compute”

P 3, col 2, para 2, ‘probability of replicating’

P 3, col 2, para 2, line 4 ‘informative about’

P 3, col 2, para 4, line 2 delete ‘of’

P 3, col 2, para 5, line 9 – ‘conditioned’ is either wrong or too technical here: would ‘based’ be acceptable as alternative wording

P 3, col 2, para 5, line 13 ‘This dichotomisation allows one to distinguish’

P 3, col 2, para 5, last sentence, delete ‘Alternatively’.

P 3, col 2, last para line 2 ‘first’

P 4, col 2, para 2, last sentence is hard to understand; not sure if this is better: ‘If sample sizes differ between studies, the distribution of CIs cannot be specified a priori’

P 5, col 1, para 2, ‘a pattern of order’ – I did not understand what was meant by this

P 5, col 1, para 2, last sentence unclear: possible rewording: “If the goal is to test the size of an effect then NHST is not the method of choice, since testing can only reject the null hypothesis.’ (??)

P 5, col 1, para 3, line 1 delete ‘that’

P 5, col 1, para 3, line 3 ‘on’ -> ‘by’

P 5, col 2, para 1, line 4 , rather than ‘Here I propose to adopt’ I suggest ‘I recommend adopting’

P 5, col 2, para 1, line 13 ‘with’ -> ‘by’

P 5, col 2, para 1 – recommend deleting last sentence

P 5, col 2, para 2, line 2 ‘consider’ -> ‘anticipate’

P 5, col 2, para 2, delete ‘should always be included’

P 5, col 2, para 2, ‘type one’ -> ‘Type I’

I have read this submission. I believe that I have an appropriate level of expertise to confirm that it is of an acceptable scientific standard, however I have significant reservations, as outlined above.

The University of Edinburgh, UK

I wondered about changing the focus slightly and modifying the title to reflect this to say something like: Null hypothesis significance testing: a guide to commonly misunderstood concepts and recommendations for good practice

Thank you for the suggestion – you indeed saw the intention behind the ‘tutorial’ style of the paper.

  • P 3, col 1, para 3, last sentence. Although statisticians always emphasise the arbitrary nature of p < .05, we all know that in practice authors who use other values are likely to have their analyses queried. I wondered whether it would be useful here to note that in some disciplines different cutoffs are traditional, e.g. particle physics. Or you could cite David Colquhoun’s paper in which he recommends using p < .001 ( http://rsos.royalsocietypublishing.org/content/1/3/140216)  - just to be clear that the traditional p < .05 has been challenged.

I have added a sentence on this citing Colquhoun 2014 and the new Benjamin 2017 on using .005.

I agree that this point is always hard to appreciate, especially because it seems like in practice it makes little difference. I added a paragraph but using reaction times rather than a coin toss – thanks for the suggestion.

Added an example based on new table 1, following figure 1 – giving CI, equivalence tests and Bayes Factor (with refs to easy to use tools)

Changed builds to constructs (this simply means they are something we build) and added that the implication that probability coverage is not warranty when sample size change, is that we cannot compare CI.

I changed ‘ i.e. we accept that 1-alpha CI are wrong in alpha percent of the times in the long run’ to ‘, ‘e.g. a 95% CI is wrong in 5% of the times in the long run (i.e. if we repeat the experiment many times).’ – for Bayesian intervals I simply re-cited Morey & Rouder, 2011.

It is not the CI cannot be specified, it’s that the interval is not predictive of anything anymore! I changed it to ‘If sample sizes, however, differ between studies, there is no warranty that a CI from one study will be true at the rate alpha in a different study, which implies that CI cannot be compared across studies at this is rarely the same sample sizes’

I added (i.e. establish that A > B) – we test that conditions are ordered, but without further specification of the probability of that effect nor its size

Yes it works – thx

P 5, col 2, para 2, ‘type one’ -> ‘Type I’ 

Typos fixed, and suggestions accepted – thanks for that.

Stephen J. Senn

1 Luxembourg Institute of Health, Strassen, L-1445, Luxembourg

The revisions are OK for me, and I have changed my status to Approved.

I have read this submission. I believe that I have an appropriate level of expertise to confirm that it is of an acceptable scientific standard.

Referee response for version 2

On the whole I think that this article is reasonable, my main reservation being that I have my doubts on whether the literature needs yet another tutorial on this subject.

A further reservation I have is that the author, following others, stresses what in my mind is a relatively unimportant distinction between the Fisherian and Neyman-Pearson (NP) approaches. The distinction stressed by many is that the NP approach leads to a dichotomy accept/reject based on probabilities established in advance, whereas the Fisherian approach uses tail area probabilities calculated from the observed statistic. I see this as being unimportant and not even true. Unless one considers that the person carrying out a hypothesis test (original tester) is mandated to come to a conclusion on behalf of all scientific posterity, then one must accept that any remote scientist can come to his or her conclusion depending on the personal type I error favoured. To operate the results of an NP test carried out by the original tester, the remote scientist then needs to know the p-value. The type I error rate is then compared to this to come to a personal accept or reject decision (1). In fact Lehmann (2), who was an important developer of and proponent of the NP system, describes exactly this approach as being good practice. (See Testing Statistical Hypotheses, 2nd edition P70). Thus using tail-area probabilities calculated from the observed statistics does not constitute an operational difference between the two systems.

A more important distinction between the Fisherian and NP systems is that the former does not use alternative hypotheses(3). Fisher's opinion was that the null hypothesis was more primitive than the test statistic but that the test statistic was more primitive than the alternative hypothesis. Thus, alternative hypotheses could not be used to justify choice of test statistic. Only experience could do that.

Further distinctions between the NP and Fisherian approach are to do with conditioning and whether a null hypothesis can ever be accepted.

I have one minor quibble about terminology. As far as I can see, the author uses the usual term 'null hypothesis' and the eccentric term 'nil hypothesis' interchangeably. It would be simpler if the latter were abandoned.

Referee response for version 1

Marcel alm van assen.

1 Department of Methodology and Statistics, Tilburgh University, Tilburg, Netherlands

Null hypothesis significance testing (NHST) is a difficult topic, with misunderstandings arising easily. Many texts, including basic statistics books, deal with the topic, and attempt to explain it to students and anyone else interested. I would refer to a good basic text book, for a detailed explanation of NHST, or to a specialized article when wishing an explaining the background of NHST. So, what is the added value of a new text on NHST? In any case, the added value should be described at the start of this text. Moreover, the topic is so delicate and difficult that errors, misinterpretations, and disagreements are easy. I attempted to show this by giving comments to many sentences in the text.

Abstract: “null hypothesis significance testing is the statistical method of choice in biological, biomedical and social sciences to investigate if an effect is likely”. No, NHST is the method to test the hypothesis of no effect.

Intro: “Null hypothesis significance testing (NHST) is a method of statistical inference by which an observation is tested against a hypothesis of no effect or no relationship.” What is an ‘observation’? NHST is difficult to describe in one sentence, particularly here. I would skip this sentence entirely, here.

Section on Fisher; also explain the one-tailed test.

Section on Fisher; p(Obs|H0) does not reflect the verbal definition (the ‘or more extreme’ part).

Section on Fisher; use a reference and citation to Fisher’s interpretation of the p-value

Section on Fisher; “This was however only intended to be used as an indication that there is something in the data that deserves further investigation. The reason for this is that only H0 is tested whilst the effect under study is not itself being investigated.” First sentence, can you give a reference? Many people say a lot about Fisher’s intentions, but the good man is dead and cannot reply… Second sentence is a bit awkward, because the effect is investigated in a way, by testing the H0.

Section on p-value; Layout and structure can be improved greatly, by first again stating what the p-value is, and then statement by statement, what it is not, using separate lines for each statement. Consider adding that the p-value is randomly distributed under H0 (if all the assumptions of the test are met), and that under H1 the p-value is a function of population effect size and N; the larger each is, the smaller the p-value generally is.

Skip the sentence “If there is no effect, we should replicate the absence of effect with a probability equal to 1-p”. Not insightful, and you did not discuss the concept ‘replicate’ (and do not need to).

Skip the sentence “The total probability of false positives can also be obtained by aggregating results ( Ioannidis, 2005 ).” Not strongly related to p-values, and introduces unnecessary concepts ‘false positives’ (perhaps later useful) and ‘aggregation’.

Consider deleting; “If there is an effect however, the probability to replicate is a function of the (unknown) population effect size with no good way to know this from a single experiment ( Killeen, 2005 ).”

The following sentence; “ Finally, a (small) p-value  is not an indication favouring a hypothesis . A low p-value indicates a misfit of the null hypothesis to the data and cannot be taken as evidence in favour of a specific alternative hypothesis more than any other possible alternatives such as measurement error and selection bias ( Gelman, 2013 ).” is surely not mainstream thinking about NHST; I would surely delete that sentence. In NHST, a p-value is used for testing the H0. Why did you not yet discuss significance level? Yes, before discussing what is not a p-value, I would explain NHST (i.e., what it is and how it is used). 

Also the next sentence “The more (a priori) implausible the alternative hypothesis, the greater the chance that a finding is a false alarm ( Krzywinski & Altman, 2013 ;  Nuzzo, 2014 ).“ is not fully clear to me. This is a Bayesian statement. In NHST, no likelihoods are attributed to hypotheses; the reasoning is “IF H0 is true, then…”.

Last sentence: “As  Nickerson (2000)  puts it ‘theory corroboration requires the testing of multiple predictions because the chance of getting statistically significant results for the wrong reasons in any given case is high’.” What is relation of this sentence to the contents of this section, precisely?

Next section: “For instance, we can estimate that the probability of a given F value to be in the critical interval [+2 +∞] is less than 5%” This depends on the degrees of freedom.

“When there is no effect (H0 is true), the erroneous rejection of H0 is known as type I error and is equal to the p-value.” Strange sentence. The Type I error is the probability of erroneously rejecting the H0 (so, when it is true). The p-value is … well, you explained it before; it surely does not equal the Type I error.

Consider adding a figure explaining the distinction between Fisher’s logic and that of Neyman and Pearson.

“When the test statistics falls outside the critical region(s)” What is outside?

“There is a profound difference between accepting the null hypothesis and simply failing to reject it ( Killeen, 2005 )” I agree with you, but perhaps you may add that some statisticians simply define “accept H0’” as obtaining a p-value larger than the significance level. Did you already discuss the significance level, and it’s mostly used values?

“To accept or reject equally the null hypothesis, Bayesian approaches ( Dienes, 2014 ;  Kruschke, 2011 ) or confidence intervals must be used.” Is ‘reject equally’ appropriate English? Also using Cis, one cannot accept the H0.

Do you start discussing alpha only in the context of Cis?

“CI also indicates the precision of the estimate of effect size, but unless using a percentile bootstrap approach, they require assumptions about distributions which can lead to serious biases in particular regarding the symmetry and width of the intervals ( Wilcox, 2012 ).” Too difficult, using new concepts. Consider deleting.

“Assuming the CI (a)symmetry and width are correct, this gives some indication about the likelihood that a similar value can be observed in future studies, with 95% CI giving about 83% chance of replication success ( Lakens & Evers, 2014 ).” This statement is, in general, completely false. It very much depends on the sample sizes of both studies. If the replication study has a much, much, much larger N, then the probability that the original CI will contain the effect size of the replication approaches (1-alpha)*100%. If the original study has a much, much, much larger N, then the probability that the original Ci will contain the effect size of the replication study approaches 0%.

“Finally, contrary to p-values, CI can be used to accept H0. Typically, if a CI includes 0, we cannot reject H0. If a critical null region is specified rather than a single point estimate, for instance [-2 +2] and the CI is included within the critical null region, then H0 can be accepted. Importantly, the critical region must be specified a priori and cannot be determined from the data themselves.” No. H0 cannot be accepted with Cis.

“The (posterior) probability of an effect can however not be obtained using a frequentist framework.” Frequentist framework? You did not discuss that, yet.

“X% of times the CI obtained will contain the same parameter value”. The same? True, you mean?

“e.g. X% of the times the CI contains the same mean” I do not understand; which mean?

“The alpha value has the same interpretation as when using H0, i.e. we accept that 1-alpha CI are wrong in alpha percent of the times. “ What do you mean, CI are wrong? Consider rephrasing.

“To make a statement about the probability of a parameter of interest, likelihood intervals (maximum likelihood) and credibility intervals (Bayes) are better suited.” ML gives the likelihood of the data given the parameter, not the other way around.

“Many of the disagreements are not on the method itself but on its use.” Bayesians may disagree.

“If the goal is to establish the likelihood of an effect and/or establish a pattern of order, because both requires ruling out equivalence, then NHST is a good tool ( Frick, 1996 )” NHST does not provide evidence on the likelihood of an effect.

“If the goal is to establish some quantitative values, then NHST is not the method of choice.” P-values are also quantitative… this is not a precise sentence. And NHST may be used in combination with effect size estimation (this is even recommended by, e.g., the American Psychological Association (APA)).

“Because results are conditioned on H0, NHST cannot be used to establish beliefs.” It can reinforce some beliefs, e.g., if H0 or any other hypothesis, is true.

“To estimate the probability of a hypothesis, a Bayesian analysis is a better alternative.” It is the only alternative?

“Note however that even when a specific quantitative prediction from a hypothesis is shown to be true (typically testing H1 using Bayes), it does not prove the hypothesis itself, it only adds to its plausibility.” How can we show something is true?

I do not agree on the contents of the last section on ‘minimal reporting’. I prefer ‘optimal reporting’ instead, i.e., the reporting the information that is essential to the interpretation of the result, to any ready, which may have other goals than the writer of the article. This reporting includes, for sure, an estimate of effect size, and preferably a confidence interval, which is in line with recommendations of the APA.

I have read this submission. I believe that I have an appropriate level of expertise to state that I do not consider it to be of an acceptable scientific standard, for reasons outlined above.

The idea of this short review was to point to common interpretation errors (stressing again and again that we are under H0) being in using p-values or CI, and also proposing reporting practices to avoid bias. This is now stated at the end of abstract.

Regarding text books, it is clear that many fail to clearly distinguish Fisher/Pearson/NHST, see Glinet et al (2012) J. Exp Education 71, 83-92. If you have 1 or 2 in mind that you know to be good, I’m happy to include them.

I agree – yet people use it to investigate (not test) if an effect is likely. The issue here is wording. What about adding this distinction at the end of the sentence?: ‘null hypothesis significance testing is the statistical method of choice in biological, biomedical and social sciences used to investigate if an effect is likely, even though it actually tests for the hypothesis of no effect’.

I think a definition is needed, as it offers a starting point. What about the following: ‘NHST is a method of statistical inference by which an experimental factor is tested against a hypothesis of no effect or no relationship based on a given observation’

The section on Fisher has been modified (more or less) as suggested: (1) avoiding talking about one or two tailed tests (2) updating for p(Obs≥t|H0) and (3) referring to Fisher more explicitly (ie pages from articles and book) ; I cannot tell his intentions but these quotes leave little space to alternative interpretations.

The reasoning here is as you state yourself, part 1: ‘a p-value is used for testing the H0; and part 2: ‘no likelihoods are attributed to hypotheses’ it follows we cannot favour a hypothesis. It might seems contentious but this is the case that all we can is to reject the null – how could we favour a specific alternative hypothesis from there? This is explored further down the manuscript (and I now point to that) – note that we do not need to be Bayesian to favour a specific H1, all I’m saying is this cannot be attained with a p-value.

The point was to emphasise that a p value is not there to tell us a given H1 is true and can only be achieved through multiple predictions and experiments. I deleted it for clarity.

This sentence has been removed

Indeed, you are right and I have modified the text accordingly. When there is no effect (H0 is true), the erroneous rejection of H0 is known as type 1 error. Importantly, the type 1 error rate, or alpha value is determined a priori. It is a common mistake but the level of significance (for a given sample) is not the same as the frequency of acceptance alpha found on repeated sampling (Fisher, 1955).

A figure is now presented – with levels of acceptance, critical region, level of significance and p-value.

I should have clarified further here – as I was having in mind tests of equivalence. To clarify, I simply states now: ‘To accept the null hypothesis, tests of equivalence or Bayesian approaches must be used.’

It is now presented in the paragraph before.

Yes, you are right, I completely overlooked this problem. The corrected sentence (with more accurate ref) is now “Assuming the CI (a)symmetry and width are correct, this gives some indication about the likelihood that a similar value can be observed in future studies. For future studies of the same sample size, 95% CI giving about 83% chance of replication success (Cumming and Mallardet, 2006). If sample sizes differ between studies, CI do not however warranty any a priori coverage”.

Again, I had in mind equivalence testing, but in both cases you are right we can only reject and I therefore removed that sentence.

Yes, p-values must be interpreted in context with effect size, but this is not what people do. The point here is to be pragmatic, does and don’t. The sentence was changed.

Not for testing, but for probability, I am not aware of anything else.

Cumulative evidence is, in my opinion, the only way to show it. Even in hard science like physics multiple experiments. In the recent CERN study on finding Higgs bosons, 2 different and complementary experiments ran in parallel – and the cumulative evidence was taken as a proof of the true existence of Higgs bosons.

Daniel Lakens

1 School of Innovation Sciences, Eindhoven University of Technology, Eindhoven, Netherlands

I appreciate the author's attempt to write a short tutorial on NHST. Many people don't know how to use it, so attempts to educate people are always worthwhile. However, I don't think the current article reaches it's aim. For one, I think it might be practically impossible to explain a lot in such an ultra short paper - every section would require more than 2 pages to explain, and there are many sections. Furthermore, there are some excellent overviews, which, although more extensive, are also much clearer (e.g., Nickerson, 2000 ). Finally, I found many statements to be unclear, and perhaps even incorrect (noted below). Because there is nothing worse than creating more confusion on such a topic, I have extremely high standards before I think such a short primer should be indexed. I note some examples of unclear or incorrect statements below. I'm sorry I can't make a more positive recommendation.

“investigate if an effect is likely” – ambiguous statement. I think you mean, whether the observed DATA is probable, assuming there is no effect?

The Fisher (1959) reference is not correct – Fischer developed his method much earlier.

“This p-value thus reflects the conditional probability of achieving the observed outcome or larger, p(Obs|H0)” – please add 'assuming the null-hypothesis is true'.

“p(Obs|H0)” – explain this notation for novices.

“Following Fisher, the smaller the p-value, the greater the likelihood that the null hypothesis is false.”  This is wrong, and any statement about this needs to be much more precise. I would suggest direct quotes.

“there is something in the data that deserves further investigation” –unclear sentence.

“The reason for this” – unclear what ‘this’ refers to.

“ not the probability of the null hypothesis of being true, p(H0)” – second of can be removed?

“Any interpretation of the p-value in relation to the effect under study (strength, reliability, probability) is indeed

wrong, since the p-value is conditioned on H0”  - incorrect. A big problem is that it depends on the sample size, and that the probability of a theory depends on the prior.

“If there is no effect, we should replicate the absence of effect with a probability equal to 1-p.” I don’t understand this, but I think it is incorrect.

“The total probability of false positives can also be obtained by aggregating results (Ioannidis, 2005).” Unclear, and probably incorrect.

“By failing to reject, we simply continue to assume that H0 is true, which implies that one cannot, from a nonsignificant result, argue against a theory” – according to which theory? From a NP perspective, you can ACT as if the theory is false.

“(Lakens & Evers, 2014”) – we are not the original source, which should be cited instead.

“ Typically, if a CI includes 0, we cannot reject H0.”  - when would this not be the case? This assumes a CI of 1-alpha.

“If a critical null region is specified rather than a single point estimate, for instance [-2 +2] and the CI is included within the critical null region, then H0 can be accepted.” – you mean practically, or formally? I’m pretty sure only the former.

The section on ‘The (correct) use of NHST’ seems to conclude only Bayesian statistics should be used. I don’t really agree.

“ we can always argue that effect size, power, etc. must be reported.” – which power? Post-hoc power? Surely not? Other types are unknown. So what do you mean?

The recommendation on what to report remains vague, and it is unclear why what should be reported.

This sentence was changed, following as well the other reviewer, to ‘null hypothesis significance testing is the statistical method of choice in biological, biomedical and social sciences to investigate if an effect is likely, even though it actually tests whether the observed data are probable, assuming there is no effect’

Changed, refers to Fisher 1925

I changed a little the sentence structure, which should make explicit that this is the condition probability.

This has been changed to ‘[…] to decide whether the evidence is worth additional investigation and/or replication (Fisher, 1971 p13)’

my mistake – the sentence structure is now ‘ not the probability of the null hypothesis p(H0), of being true,’ ; hope this makes more sense (and this way refers back to p(Obs>t|H0)

Fair enough – my point was to stress the fact that p value and effect size or H1 have very little in common, but yes that the part in common has to do with sample size. I left the conditioning on H0 but also point out the dependency on sample size.

The whole paragraph was changed to reflect a more philosophical take on scientific induction/reasoning. I hope this is clearer.

Changed to refer to equivalence testing

I rewrote this, as to show frequentist analysis can be used  - I’m trying to sell Bayes more than any other approach.

I’m arguing we should report it all, that’s why there is no exhausting list – I can if needed.

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null hypothesis in research paper example

Null Hypothesis

The null hypothesis, H 0 , is an essential part of any research design, and is always tested, even indirectly.

This article is a part of the guide:

  • Research Hypothesis
  • Defining a Research Problem
  • Selecting Method
  • Test Hypothesis

Browse Full Outline

  • 1 Scientific Method
  • 2.1.1 Null Hypothesis
  • 2.1.2 Research Hypothesis
  • 2.2 Prediction
  • 2.3 Conceptual Variable
  • 3.1 Operationalization
  • 3.2 Selecting Method
  • 3.3 Measurements
  • 3.4 Scientific Observation
  • 4.1 Empirical Evidence
  • 5.1 Generalization
  • 5.2 Errors in Conclusion

The simplistic definition of the null is as the opposite of the alternative hypothesis , H 1 , although the principle is a little more complex than that.

The null hypothesis (H 0 ) is a hypothesis which the researcher tries to disprove, reject or nullify.

The 'null' often refers to the common view of something, while the alternative hypothesis is what the researcher really thinks is the cause of a phenomenon.

An experiment conclusion always refers to the null, rejecting or accepting H 0 rather than H 1 .

Despite this, many researchers neglect the null hypothesis when testing hypotheses , which is poor practice and can have adverse effects.

null hypothesis in research paper example

Examples of the Null Hypothesis

A researcher may postulate a hypothesis:

H 1 : Tomato plants exhibit a higher rate of growth when planted in compost rather than in soil.

And a null hypothesis:

H 0 : Tomato plants do not exhibit a higher rate of growth when planted in compost rather than soil.

It is important to carefully select the wording of the null, and ensure that it is as specific as possible. For example, the researcher might postulate a null hypothesis:

H 0 : Tomato plants show no difference in growth rates when planted in compost rather than soil.

There is a major flaw with this H 0 . If the plants actually grow more slowly in compost than in soil, an impasse is reached. H 1 is not supported, but neither is H 0 , because there is a difference in growth rates.

If the null is rejected, with no alternative, the experiment may be invalid. This is the reason why science uses a battery of deductive and inductive processes to ensure that there are no flaws in the hypotheses.

Reasoning Cycle - Scientific Research

Many scientists neglect the null, assuming that it is merely the opposite of the alternative, but it is good practice to spend a little time creating a sound hypothesis. It is not possible to change any hypothesis retrospectively, including H 0 .

null hypothesis in research paper example

Significance Tests

If significance tests generate 95% or 99% likelihood that the results do not fit the null hypothesis, then it is rejected, in favor of the alternative.

Otherwise, the null is accepted. These are the only correct assumptions, and it is incorrect to reject, or accept, H 1 .

Accepting the null hypothesis does not mean that it is true. It is still a hypothesis, and must conform to the principle of falsifiability , in the same way that rejecting the null does not prove the alternative.

Perceived Problems With the Null

The major problem with the H 0 is that many researchers, and reviewers, see accepting the null as a failure of the experiment . This is very poor science, as accepting or rejecting any hypothesis is a positive result.

Even if the null is not refuted, the world of science has learned something new. Strictly speaking, the term ‘failure’, should only apply to errors in the experimental design , or incorrect initial assumptions.

Development of the Null

The Flat Earth model was common in ancient times, such as in the civilizations of the Bronze Age or Iron Age. This may be thought of as the null hypothesis, H 0 , at the time.

H 0 : World is Flat

Many of the Ancient Greek philosophers assumed that the sun, moon and other objects in the universe circled around the Earth. Hellenistic astronomy established the spherical shape of the earth around 300 BC.

H 0 : The Geocentric Model: Earth is the centre of the Universe and it is Spherical

Copernicus had an alternative hypothesis , H 1 that the world actually circled around the sun, thus being the center of the universe. Eventually, people got convinced and accepted it as the null, H 0 .

H 0 : The Heliocentric Model: Sun is the centre of the universe

Later someone proposed an alternative hypothesis that the sun itself also circled around the something within the galaxy, thus creating a new H 0 . This is how research works - the H 0 gets closer to the reality each time, even if it isn't correct, it is better than the last H 0 .

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Martyn Shuttleworth (Feb 3, 2008). Null Hypothesis. Retrieved Aug 18, 2024 from Explorable.com: https://explorable.com/null-hypothesis

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IMAGES

  1. 15 Null Hypothesis Examples (2024)

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  3. Null Hypothesis

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  4. hypothesis in research methodology notes

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  5. Where Does The Hypothesis Go In A Research Paper

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  6. The Null Hypothesis and Research Hypothesis

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COMMENTS

  1. Null & Alternative Hypotheses

    The null and alternative hypotheses offer competing answers to your research question. When the research question asks "Does the independent variable affect the dependent variable?": The null hypothesis ( H0) answers "No, there's no effect in the population.". The alternative hypothesis ( Ha) answers "Yes, there is an effect in the ...

  2. How to Formulate a Null Hypothesis (With Examples)

    To distinguish it from other hypotheses, the null hypothesis is written as H 0 (which is read as "H-nought," "H-null," or "H-zero"). A significance test is used to determine the likelihood that the results supporting the null hypothesis are not due to chance. A confidence level of 95% or 99% is common. Keep in mind, even if the confidence level is high, there is still a small chance the ...

  3. How to Write a Null Hypothesis (5 Examples)

    H 0 (Null Hypothesis): Population parameter =, ≤, ≥ some value. H A (Alternative Hypothesis): Population parameter <, >, ≠ some value. Note that the null hypothesis always contains the equal sign. We interpret the hypotheses as follows: Null hypothesis: The sample data provides no evidence to support some claim being made by an individual.

  4. Null Hypothesis: Definition, Rejecting & Examples

    It is one of two mutually exclusive hypotheses about a population in a hypothesis test. When your sample contains sufficient evidence, you can reject the null and conclude that the effect is statistically significant. Statisticians often denote the null hypothesis as H 0 or H A. Null Hypothesis H0: No effect exists in the population.

  5. Null and Alternative Hypotheses

    The null and alternative hypotheses offer competing answers to your research question. When the research question asks "Does the independent variable affect the dependent variable?", the null hypothesis (H 0) answers "No, there's no effect in the population.". On the other hand, the alternative hypothesis (H A) answers "Yes, there ...

  6. How to Write a Null Hypothesis (with Examples and Templates)

    Write a research null hypothesis as a statement that the studied variables have no relationship to each other, or that there's no difference between 2 groups. Write a statistical null hypothesis as a mathematical equation, such as. μ 1 = μ 2 {\displaystyle \mu _ {1}=\mu _ {2}} if you're comparing group means.

  7. Crafting a Null Hypothesis: A Guide to Writing it Right

    The null hypothesis is denoted as H0 and is a critical component in how to write a thesis or a research paper. Formulating the Alternate Hypothesis The alternate hypothesis (H1) represents what you're trying to demonstrate, such as a significant effect or difference between groups.

  8. Null Hypothesis Examples

    An example of the null hypothesis is that light color has no effect on plant growth. The null hypothesis (H 0) is the hypothesis that states there is no statistical difference between two sample sets. In other words, it assumes the independent variable does not have an effect on the dependent variable in a scientific experiment.

  9. How to Write a Strong Hypothesis

    Developing a hypothesis (with example) Step 1. Ask a question. Writing a hypothesis begins with a research question that you want to answer. The question should be focused, specific, and researchable within the constraints of your project. Example: Research question.

  10. Null Hypothesis Definition and Examples

    Null Hypothesis Examples. "Hyperactivity is unrelated to eating sugar " is an example of a null hypothesis. If the hypothesis is tested and found to be false, using statistics, then a connection between hyperactivity and sugar ingestion may be indicated. A significance test is the most common statistical test used to establish confidence in a ...

  11. Formulating a Null Hypothesis: Key Elements to Consider

    A null hypothesis (H0) is a statement that there is no effect or no difference, and it serves as the starting point for statistical testing. Formulating a null hypothesis involves defining a clear and concise research question, stating the hypothesis in a way that allows for empirical testing, and considering the potential for Type I errors.

  12. Null Hypothesis Definition and Examples, How to State

    Step 1: Figure out the hypothesis from the problem. The hypothesis is usually hidden in a word problem, and is sometimes a statement of what you expect to happen in the experiment. The hypothesis in the above question is "I expect the average recovery period to be greater than 8.2 weeks.". Step 2: Convert the hypothesis to math.

  13. Examples of null and alternative hypotheses

    The null hypothesis is what happens at baseline. It is the uninteresting hypothesis--the boring hypothesis. Usually, it is the hypothesis that assumes no difference. It is the opposite of your research hypothesis. The alternative hypothesis--that is, the research hypothesis--is the idea, phenomenon, observation that you want to prove.

  14. What Is The Null Hypothesis & When To Reject It

    When your p-value is less than or equal to your significance level, you reject the null hypothesis. In other words, smaller p-values are taken as stronger evidence against the null hypothesis. Conversely, when the p-value is greater than your significance level, you fail to reject the null hypothesis. In this case, the sample data provides ...

  15. Null Hypothesis

    The null hypothesis is an example of a statistical hypothesis. In order to test these hypotheses, the researcher designs a study, measures the variables of interest, and calculates a statistic, such as a mean difference, or a correlation, which acts as a sample estimate of the population parameter defined by our statistical hypothesis.

  16. 10.1

    10.1 - Setting the Hypotheses: Examples. A significance test examines whether the null hypothesis provides a plausible explanation of the data. The null hypothesis itself does not involve the data. It is a statement about a parameter (a numerical characteristic of the population). These population values might be proportions or means or ...

  17. 15 Null Hypothesis Examples (2024)

    A null hypothesis is a general assertion or default position that there is no relationship or effect between two measured phenomena. It's a critical part of statistics, data analysis, and the scientific method. This concept forms the basis of testing statistical significance and allows researchers to be objective in their conclusions.

  18. An Introduction to Statistics: Understanding Hypothesis Testing and

    HYPOTHESIS TESTING. A clinical trial begins with an assumption or belief, and then proceeds to either prove or disprove this assumption. In statistical terms, this belief or assumption is known as a hypothesis. Counterintuitively, what the researcher believes in (or is trying to prove) is called the "alternate" hypothesis, and the opposite ...

  19. Understanding Null Hypothesis Testing

    A crucial step in null hypothesis testing is finding the likelihood of the sample result if the null hypothesis were true. This probability is called the p value. A low p value means that the sample result would be unlikely if the null hypothesis were true and leads to the rejection of the null hypothesis. A high p value means that the sample ...

  20. 7.3: The Research Hypothesis and the Null Hypothesis

    The Research Hypothesis. A research hypothesis is a mathematical way of stating a research question. A research hypothesis names the groups (we'll start with a sample and a population), what was measured, and which we think will have a higher mean. The last one gives the research hypothesis a direction. In other words, a research hypothesis ...

  21. Null and Alternative Hypothesis: Research Guidelines

    Steps on How to Write a Good Null and Alternative Hypothesis. Identify a Specific Research Question: Start with clearly defining a particular problem or phenomenon you want to study. Determine Key Variables: Identify independent and dependent variables involved in your study. State a Specific Null Hypothesis (H0): Formulate a concrete statement that suggests no effect, no difference, or no ...

  22. Null and Alternative Hypotheses

    By tradition, we try to disprove (reject) the null hypothesis. We can never prove a null hypothesis, because it is impossible to prove something does not exist. We can disprove something does not exist by finding an example of it. Therefore, in research we try to disprove the null hypothesis. When we do find that a relationship (or difference ...

  23. Null hypothesis significance testing: a short tutorial

    Fisher, significance testing, and the p-value. The method developed by ( Fisher, 1934; Fisher, 1955; Fisher, 1959) allows to compute the probability of observing a result at least as extreme as a test statistic (e.g. t value), assuming the null hypothesis of no effect is true.This probability or p-value reflects (1) the conditional probability of achieving the observed outcome or larger: p(Obs ...

  24. Null Hypothesis

    The null hypothesis (H 0) is a hypothesis which the researcher tries to disprove, reject or nullify. The 'null' often refers to the common view of something, while the alternative hypothesis is what the researcher really thinks is the cause of a phenomenon. An experiment conclusion always refers to the null, rejecting or accepting H 0 rather ...