huji experiments

A database of Hfq-mediated RNA interactions determined by RIL-seq

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Institute of Earth Sciences

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Stucture and Scope: 

An MSc degree from the Institute of Earth Sciences opens doors to diverse employment opportunities in industries such as environmental consulting, natural resource management, geotechnical engineering, research institutions, government agencies, and more. Our graduates are sought after for their specialized knowledge and hands-on experience, positioning them for fulfilling careers that deal with global environmental issues.  At our institute you can specialize in one of five disciplines: Geology, Oceanography, Hydrology and Water Resources, Atmospheric Sciences, and Environmental Sciences.

The studies span two years, during which students learn 32 academic course credits, and conduct independent research under the guidance of leading scientists.

Degree Program:

Advanced mandatory and elective courses taught by top experts, providing specialized scientific knowledge and skills;

Research proposal and interim examination at the end of the first year

Written thesis and examination at the end of 2 years

Courses are chosen after consultation with the academic supervisor.

Master’s Degree in Geology (Department 590, track 6040)

Master’s Degree in Atmospheric Sciences (Department 545, track 6041)

Master’s Degree in Oceanography (Department 592, track 6042)

Master’s Degree in Environmental Sciences (Department 591, track 6090)

Master's program in Hydrology and Water Resources (Department 589, track 6044)

Specialization in Environmental Sciences for Oceanography/Geology/Atmosphere students (5904)

Research Fields

• Atmospheric Sciences
• Envirinmental Sciences
• Oceanography
• Hydrology and Water Resources

Geological studies at the Hebrew University invites you to delve into a world of research and discovery, offering a fundamental understanding of Earth and its processes. Our Master's program combines theoretical knowledge with extensive practical experience, preparing graduates for a wide array of research and employment opportunities. Key research areas include seismology and earthquakes, carbon capture and storage, volcanism, landscape formation processes, paleoclimate, geochemistry, geophysics, and more. 

For the research groups in the field of Geology 

Advisor: Prof. Ron Shaar |  [email protected]

Tackling the climate crisis requires basic research, necessitating the development and utilization of advanced tools and models in atmospheric sciences. Throughout the program, you will learn cutting edge methods for understanding and researching chemical and physical processes in the atmosphere, such as the impact of air pollution on rain formation processes, strategies for enhancing precipitation, prediction of extreme weather events, cloud dynamics, radiation transfer, aerosol effects on global warming, and more.

For the research groups in Atmospheric Sciences

Advisor: Prof.  Chaim I. Garfinkel  |  [email protected]

The growing awareness of environmental issues such as climate change, renewable energy, air pollution, and waste management highlights the need for experts with advanced knowledge and tools to develop innovative solutions and influence policy. Our program utilizes tools from chemistry, physics, and biology to investigate and understand urgent environmental challenges. Research in Environmental Sciences facilitates collaboration between leading researchers from the Institute of Earth Sciences and other research institutes. You can tailor your studies by selecting courses from various fields according to your background and research topic. Scholarships are available for Environmental Sciences Master's students, with details announced periodically.

 For the research groups in Environmental Sciences

Advisor: Prof. Simon Emmanuel |  [email protected]  

Oceans cover over 70% of the Earth's surface. They play a crucial role in the climate system, and serve as a vital source of natural resources and food. Oceanography encompasses diverse fields such as changes in the chemical composition of the sea, the impact of climate change, carbon capture and storage, the preservation of coral reefs and habitats, the interaction between the sea and atmosphere and more. Research in the department combines theoretical methods with laboratory and field experiments. You will have the opportunity to collaborate and conduct research at the Inter-University Institute in Eilat.

For the research groups in the field of oceanography

Advisor: Prof. Adi Torfstein |  [email protected]

The program in Hydrology and Water Resources offers an interdisciplinary approach that aims to equip students with strong analytical skills and enable them to study the world’s hydrosphere. Research areas include flood dynamics, contaminant transport in groundwater, and water resource management. Research methodologies include laboratory experiments, fieldwork, and advanced modeling techniques, and students can select courses from various disciplines such as geology, meteorology, environmental sciences, and soil and water studies.

For the research groups in the field of Hydrology

Advisor: Prof. Efrat Morin |  [email protected]

Requirements for Admission:

Identify a suitable supervisor (link to search for a supervisor)

Bachelor's Degree grade average of at least 85;

Candidates with an average grade between 80 and 85 will be accepted only with the approval of the Institute's Teaching Committee and will be required to take supplementary courses according to their undergraduate degree and the recommendations of the department head. 

Candidates with an average grade between 75 and 80, will be able to be admitted to a master's degree preparation year.

A university level exemption in English is required.

Registration:

Registration for a Master’s Degree is carried out in two stages:

Through the online registration at the university website

Registration with the Science Faculty by creating an account in the registration system , followed by filling out personal and academic details. After completing the registration process, proceed to the "Submission Status" page and follow the instructions.

Candidates who studied for a Bachelor’s degree at the Hebrew University can attach an unofficial grade sheet (from the personal information);

Candidates who have not yet received confirmation of eligibility for a graduate degree, should only attach a grade sheet that includes the average for the degree only.

For further information, please contact us by phone at 02-6586722 or by email at [email protected]

Scholarships and Positions

Students who devote most of their time to research are entitled to a monthly stipend, in addition to an exemption from tuition fees and the possibility of employment as teaching assistants.

Details of eligibility:

Full exemption from tuition fees;

Minimum monthly stipend of NIS 4500;

Travel funding: the institute supports the funding of travel to conferences and research trips in Israel and abroad;

Possibility of employment as a teaching assistant, subject to submission of an application to the teaching secretary and her approval. Please contact Magiuy Perkin   [email protected]  

Scholarship conditions are valid for 4 semesters (including holidays).

Changes in conditions may occur based on agreements between the supervisor and the student.

Direct path to a doctorate

Master's degree students who wish to continue their studies towards a doctorate may switch to a direct doctorate track. Transitioning to a doctoral program in this way means that research done during the master's degree can count towards your PhD thesis.

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Diffusion NMR

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Use our NMR service to measure diffusion.

Diffusion NMR experiments resolve different compounds spectroscopically in a mixture based on their differing diffusion coefficients, depending on the size and shape of the molecules. Diffusion NMR may be used to resolve otherwise intractable spectra of mixtures or it may be used to determine the size of molecules and aggregates, determining the degree of polymerization, size of a solvation shell or other microscopic structure. The spectra produced resemble chromatograms is some respects while also providing NMR information that can be used for assignment of individual components. For most mixtures though, the resolution is not sufficient for assignment and NMR chromatography techniques need to be applied.

What you should already know before continuing to read this:

Before you continue reading about diffusion NMR, you should have some knowledge of:

• The basis of NMR spectroscopy
• Pulse sequences and the vector model

To understand the section on 3D-DOSY , you should have some knowledge of:

• Heteronuclear correlation

If you want to read about these subjects first, please go to the links above.

The diffusion NMR technique is often referred to as Self-Diffusion (SD)-NMR or Diffusion Ordered SpectroscopY (DOSY). This is achieved by combining radio-frequency pulses as used in routine NMR spectroscopy with magnetic field gradients that encode spatial information.

In the simplest form of the pulsed gradient diffusion experiment (more sophisticated experiments will be described later ), called the pulsed field gradient echo (PGSE, fig. 1), the magnetization is excited with a 90° radiofrequency pulse then dispersed using a magnetic field gradient pulse . After a period of Δ/2 a 180° radiofrequency pulse inverts the dispersed magnetization such that after a period of Δ the magnetization is the negative of what it was following the gradient pulse. At this point, a second gradient pulse is applied to refocus the signal (fig. 2).

Fig. 1. Pulse sequence for gradient PGSE

Fig. 2. Effect of a magnetic field gradient pulse. The magnetization vector is rotates differently at different positions in the tube cancelling out the total signal. A refocusing gradient pulse can make the total signal reappear depending on its sign and intervening rf pulses.

Refocusing is only achieved for those nuclei that have not moved significantly up or down the tube. Diffusion causes some of the nuclei to move away from where their signals can be refocused thereby reducing the intensity of the resulting signal (fig. 3).

Fig. 3. Effect of diffusion combined with magnetic field gradient pulses. The physical movement of nuclei reduces the effectiveness of the refocusing pulse reducing the resulting signal strength.

The more intense and the longer the magnetic field gradient pulse, the more spatially selective it is (fig. 4) and the weaker the resulting signal. The intensity and duration of the magnetic field gradient pulse determine the distance that a nucleus can diffuse and still yield a signal.

Fig. 4. The effect of magnetic field gradient strength on signal strength is like focusing on the molecules that have not diffused out of range. The stronger the gradient the smaller the range and the weaker the gradient the larger the range.

Fig. 5. Diffusion spectrum. The peak on the left decays faster with increasing gradient strength and has a higher diffusion constant than the peaks on the right.

Fig. 6. Gaussian fit to diffusion peak intensity using a non-linear fit

The diffusion rate can also be calculated by a linear fit to ln( I ) versus g 2 (fig. 7). In this case, a value of 3.13 × 10 –9 m 2 s –1 is obtained. This is very similar and easier to calculate but less accurate than the non-linear fit because the points are not evenly distributed and the accuracy of ln( I ) is not the same for each point.

Fig. 7. Linear fit to diffusion peak intensity

Often signals overlap or arise from multiple environments. In such cases, a bigaussian or polygaussian decay results. This can be analyzed using a non-linear fit and does not lend itself to linear analysis. In fig. 8 the red and green Gaussian curves add to fit the experimental data.

Fig. 8. Bigaussian fit to diffusion peak intensity using a non-linear fit

Fig. 9. ILT of a bigaussian decay converted to diffusion constants and plotted on a logarithmic scale

Diffusion spectra are usually presented as a 2D plot with chemical shift on the horizontal axis and log(Diffusion constant) on the vertical axis (fig. 10). This representation is called Diffusion Ordered SpectroscopY (DOSY). The acquisition dimension is easily analyzed by a Fourier transform yielding high resolution in the frequency domain. However, analysis of the diffusion dimension involves an inversion of the Laplace transform (ILT). While this is quite accurate at up to 2% for a single decay, it has very low resolution when separating two or more overlapping signals with little chance of resolving diffusions of signals that have overlapping frequencies that differ by less than 30–50%.

Fig. 10. DOSY spectrum of norcamphor and β -cyclodextrin in DMSO- d 6

Each slice of the DOSY spectrum corresponds to the regular NMR spectrum of one component (fig. 11). The diffusion rate of the components can be read of the DOSY spectrum (fig. 10 and table 1).

Fig. 11. Individual spectra extracted from a DOSY spectrum of norcamphor and β -cyclodextrin in DMSO- d 6

Table 1. Diffusion rates measured from the DOSY spectrum of norcamphor and β -cyclodextrin in DMSO- d 6

Size measurement using diffusion

The self-diffusion constant is measured in m 2 s -1 and is larger for smaller molecules and less viscous solvents than it is for large molecules and viscous media (fig. 12). For example, at 25°C the self-diffusion constant of water is 2.299 × 10 -9 m 2 s -1 . For the less viscous acetone, it is 4.57 × 10 -9 m 2 s -1 while for the more viscous and larger octan-1-ol it is 1.4 × 10 -10 m 2 s -1 . Our equipment enables us to diffusion constants in the range 10 -7 to 10 -14 m 2 s -1 . The molecular size can be estimated from the Stokes-Einstein equation, where r is the van der Waals radius of the molecule in meters, k is the Boltzmann constant (1.380 × 10 -23 J K -1 ), T is the temperature in Kelvin, η is the viscosity of the solution in Pascal seconds (Pa s = 1000 centipoises) and D is the self-diffusion constant. For example, the self-diffusion constant of 9,10-diphenylanthracene in THF- d 8 at 25°C is 1.04 × 10 -9 m 2 s -1 and the viscosity is 0.501 mPa s. Applying the Stokes equation, the radius is calculated to be 0.42 nm, comparing well with the measured mean van der Waals radius of 0.41 nm.

Fig. 12. The effect of diffusion rate on signal strength is like focusing on the molecules that have not diffused out of range. Small molecules diffuse quickly leaving few nuclei that refocus while large molecules diffuse slowly leaving more nuclei in place yielding a stronger signal.

However, the Stokes-Einstein equation assumes spherical molecules much larger than the solvent molecules. Small molecules diffuse faster than expected while large planar molecules diffuse slower than expected. In the above case, the small size of the molecule relative to THF- d 8 is counteracted by its planarity giving a near perfect result (fig. 13). In ionic solutions, the effective diffusion radius is extended by a solvent shell becoming significantly larger than the van der Waals radius.

Fig. 13. Comparison of the molecular size calculated from the Stokes-Einstein equation with the van der Waals radius

R. E. Hoffman, et al. , J. Chem. Soc. Perkin 2 , 1998, 1659-1664.

Stokes-Einstein equation can be more successfully applied to larger entities such as micelles that are usually spherical (fig. 14). However, if there is a significant difference in magnetic susceptibility inside and outside the droplets, the magnetic field of the spectrometer will distort the micelles and the diffusion rate will vary with gradient direction.

Fig. 14. Comparison of the emulsion droplet size calculated from the Stokes-Einstein equation with that determined by other methods

J.P.N. Duynhoven, et al. , Magn. Reson. Chem. , 2002, 40 , S51-S59.

Another use for diffusion is the study of phase mobility in complex liquids. For example, an emulsion of oil and water can exist in three states: water in oil (W/O), bicontinuous or oil in water (O/W). A substrate that is hydrophilic ( i. e. , dissolves better in water) will diffuse much faster in an O/W or bicontinuous emulsion than in a W/O emulsion. Conversely, a substrate that is hydrophobic ( i. e. , dissolves better in oil) will diffuse much faster in an W/O or bicontinuous emulsion than in a O/W emulsion. A combination of the diffusion constants of both species indicates the state of the emulsion (fig. 15).

Fig. 15. Diffusion constants of substrates in water (circles) and oil (triangles) as a function of dilution used to differentiate three emulsion states: W/O, bicontinuous and O/W

A. Spernath, et al. , J. Agric. Food Chem. , 2003, 51 , 2359-2364.

Diffusion spectra can be combined with any 2D technique in order to separate 2D spectra in the diffusion dimension. The advantage is that there is greater dispersion of the signals reducing the need to resolve overlapping signals in the diffusion dimension, thereby increasing the accuracy of the diffusion measurement. For example, a DOSY-TOCSY is a 3D spectrum (fig. 16) whose projections are DOSY and TOCSY spectra while its slices are individual TOCSY spectra of each molecule (fig. 17).

Fig. 16. 3D-DOSY-TOCSY spectrum of a mixture of β -cyclodextrin, norcamphor in DMSO- d 6

To see this figure correctly place a red filter of the left eye and a cyan filter over the right eye.

Fig. 17. Projections and slices of a 3D-DOSY-TOCSY spectrum of a mixture of β -cyclodextrin, norcamphor in DMSO- d 6

Heteronuclear correlation (in this case HSQC) between 1 H and 13 C has the advantage of greatly increased dispersion along the 13 C axis that reduces the adverse effects of overlap when combined with DOSY (Fig. 18). However, the low isotopic abundance of 13 C reduces sensitivity. Nonetheless, for concentrated samples, excellent resolution compounds (fig. 19) outweighs the sensitivity issue.

Fig. 18. DOSY-HSQC spectrum of a mixture of β -cyclodextrin and norcamphor in DMSO- d 6

Fig. 19. Projections and slices of a DOSY-HSQC spectrum of a mixture of β -cyclodextrin and norcamphor in DMSO- d 6

DOSY can be also combined with other 2D NMR methods such as COSY , NOESY and ROESY .

Experimental conditions and methods

For best results care must be taken that the gradients are shielded and that the pre-emphasis is correctly adjusted to yield a pulse of the expected shape, that the temperature is stable and that thermal convection is not occurring. Although the shape of the magnetic field gradient pulses (fig. 20) in the diagrams is rectangular, the sudden gradient switching causes artifacts. Sine shaped pulses are commonly used although they are about a third less intense. A compromise between smoothness is the smoothed square pulse that may be necessary for measuring slow diffusion. If the experimental conditions are not set up carefully, the decay curve will look decidedly ungaussian and even wavy and the diffusion rate will appear faster than it should. Sensitivity will be lost and the wrong value of the diffusion constant will be obtained.

Fig. 20. Magnetic field gradient pulse shapes

There are four main methods used for such measurements: Pulsed Gradient Spin Echo (PGSE), Pulsed Field Gradient Stimulated Spin Echo (PFG-SSE or PFG-STE), bipolar pulse longitudinal eddy current delay (BPP-LED) and asymmetric bipolar PFG-SSE that is popularly referred to as 'oneshot' because for technical reasons it can be acquired more quickly than the others.

PGSE (fig. 1 above) is the simplest pulsed field gradient NMR method or measuring diffusion and best suited to spectra consisting only of singlets where the transverse relaxation ( T 2 ) is not much faster than the longitudinal relaxation ( T 1 ) .

In most cases, T 2 is much shorter than T 1 and PFG-SSE (fig. 21) yields much more sensitivity than PGSE even though half the theoretically available signal is lost. When the spectrum contains multiplets, PGSE severely distorts them so PFG-SSE is a must. The pulse sequence first excites the magnetization then disperses its phase with a magnetic field gradient pulse . A second radiofrequency pules is applied that moves half of the dispersed magnetization onto the z -axis. After a delay that allows diffusion to occur, a third radiofrequency pulse returns the dispersed magnetization to the x , y -plane where a final magnetic field gradient pulse refocuses it to yield a signal whose intensity is dependent on the diffusion rate.

Fig. 21. Pulse sequence for gradient PFG-SSE

For gradient systems such as ours (Bruker DRX 400 with BGU II gradient unit) that lack a B 0 compensation unit, bipolar pulses and eddy current reduction dramatically reduce the required phase cycling and improve the line-shape. Therefore, we use BPP-LED (fig. 22). However, for very strong gradient pulses, artifacts such as waviness in the decay curves may occur and a fully phase-cycled PFG-SSE may be required to yield an accurate result. Bipolar gradient pulses consist of two opposing gradient pulses separated by a 180° radiofrequency pulse. This has the same effect on the magnitude of the NMR signal as a single magnetic field gradient pulse but cancels out temporary perturbations in the overall magnetic field improving spectra resolution. The LED sequence adds two pulses at the end, the first moves the magnetization onto the z -axis and the second returns it to the x , y -plane a few milliseconds later, effectively storing the signal while any eddy-currents decay.

Fig. 22. Pulse sequence for gradient BPP-LED

On our 500 MHz spectrometer, there is better gradient shielding and we use the oneshot sequence (fig. 23) which is an asymmetric bipolar PFG-SSE experiment. A combination of asymmetric pulses and spoil gradients (applied while the magnetization is on the z -axis so as not to affect it) reduces the number of pulses required to obtain reliable results.

Fig. 23. Pulse sequence for oneshot

Convection in a regular 5 mm NMR tube is commonly observed at room temperature for low-viscosity solvents such as acetone and methanol or for other systems at elevated temperatures. Convection moves molecules in a different way than diffusion distorting the Gaussian decay. One solution is to use narrower (3 to 4 mm outer diameter, 0.85 to 2 mm inner diameter) regular Pyrex tubing containing the sample inserted into a 5 mm NMR tube. The narrower tube suppresses convection and we often use 4 mm outer diameter (2 mm inner diameter) tubes inside 5mm NMR tubes.

Convection compromises measurements of slow diffusion more than fast diffusion. For diffusion rates less than 10 -11 m 2 s –1 it is necessary to use a convection compensated sequence (fig. 24), even if no convection is apparent, in order to obtain accurate results. The disadvantage of this sequence is that it retains only a quarter of the signal because it uses a double gradient echo.

Fig. 24. Pulse sequence for convection-compensated oneshot

3D-DOSY pulse sequences are made by replacing the first pulse of a 2D pulse sequence (such as TOCSY ) with the DOSY sequence (fig. 25).

Fig. 25. Combination of pulse sequences to make a 3D-DOSY-TOCSY sequence. The greyed regions of the DOSY acquisition and the first pulse of the TOCSY sequence are elimination when combined.

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School of Pharmacy

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• Thermal analysis & NMR
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• The Mass Spec Unit

We offer analytical services for various applications. Services can include method development, sample preparation, data acquisition and analysis. A selection of analytical services is also offered to the general public.

Thermal Analysis lab

Thermal Analysis is a group of techniques in which a physical property of a substance is measured as a function of temperature. The Thermal Analysis lab at the Institute for Drug Research is equipped with 2 instruments:

• Differential Scanning Calorimeter - METTLER TOLEDO DSC1
• Thermogravimetric Analysis- METTLER TOLEDO TGA/DSC1

Instruments are accessible to staff of the Faculty of Medicine who has been trained on their use and has passed a practical and theoretical exam.

Thermal analysis services is available to all inside and outside users, both academic and industrial.

The lab is located at The Faculty of Medicine Ein Karem, School of Pharmacy building, (-1) floor, room 27.

Thermal Analysis Lab Instrumentation:

Differential Scanning Calorimeter (DSC)

In DSC, the heat flow in and out of a sample and a reference material is measured as a function of temperature as the sample is heated, cooled or held isothermally at constant temperature. The measurement signal is the energy absorbed or released by the sample. METTLER TOLEDO DSC 1 is used in our thermal analysis laboratories to determine Physical properties and processes like: 

• Crystallization
• Glass transition
• Melting point
• Thermal stability
• Solid-solid transitions and polymorphism
• Chemical reactions such as thermal decomposition or polymerization
• Comparison of different batches of product

METTLER TOLEDO DSC1 Temperature Range: -150°C – 700°C.

Thermogravimetric Analysis (TGA)

TGA is an experimental technique in which the mass of a sample is measured as a function of sample temperature or as a function of the time in isothermal experiments. The results of a TGA measurement are display as a curve of mass against temperature or time.  METTLER TOLEDO TGA/DSC1 instrument that is used in our thermal analysis laboratories, simultaneously measures heat flow in addition to weight change. The effects that can cause a sample to lose mass include:

• Evaporation of volatile constituents
• Loss of water of crystallization
• Oxidation of metals
• Oxidative decomposition of organic substances
• Thermal decomposition with the formation of gaseous products.

METTLER TOLEDO TGA/DSC1 Temperature Range: RT–1000°C. 

Thermal Analysis Service s

Thermal Analysis service is provided on DSC and TGA both for the Faculty of Medicine staff (Internal Services) as well as for external users, academic and industrial (External Services).

Samples may be submitted for measuring only or measuring and data analysis. The service offers specialist support and advice in thermal analysis includes:

• Sample preparation 
• Performing the right measurements 
• Interpretation of DSC curve

For price quote please contact:  Dr. Aviva Friedman-Ezra, Tel: 87030,  E-mail: [email protected]

Nuclear Magnetic Resonance (NMR) Unit

Lab instrumentation  

Bruker Fourier 80

Varian Mercury 300 MHz NMR

Bruker Avance III 500 MHz NMR

The NMR lab of the Institute for Drug Research is equipped with three NMR spectrometers including a benchtop system Bruker Fourier 80, a 300 MHz instrument and a 500 MHz instrument. Our primary goal is to support the research at the Faculty of Medicine, by providing a variety of NMR applications such as

• Chemical structure analysis for detailed structural characterization for small molecules
• Quantification of mixture components
• Kinetic studies of reaction mixtures
• Characterization of polymers

The NMR lab instruments are accessible to staff and students of the Faculty of Medicine who have been trained on their use and have passed a practical and theoretical exam. NMR services are available to all inside and outside users, both academic and industrial.

The NMR lab (NMR300, NMR500) is located at the Faculty of Medicine, building 4, (-2) floor, room 5. Bruker Fourier 80 is located at the Faculty of Medicine, School of Pharmacy building, 4th floor, Room 412

Lab Instrumentation:

• Compact benchtop system.
• Cryogen-free permanent magnet (no liquid nitrogen or liquid helium required).
• Easy-to-use, minimal training is required to perform a measurements.
• Allowing students' hands-on training.
• Standard 5mm diameter, 7’’ NMR tubes.
• Deuterated solvents are not necessary
• Suitable for:  - Assess the purity of samples  - Reaction monitoring  - Polymers characterization (determination of the molecular weight or copolymer compositions)   - Chemistry education

Is used primarily for routine identification and standard work with small organic molecules

• Equipped with a 5mm auto-switchable 4-nuclei (1H/19F/13C/31P) probe
• Suitable for preforming routine one- and two-dimensional experiments, such as : APT, DEPT, 1D-NOESY, 1D-TOCSY, J-RESOLVE, COSY, NOESY, TOCSY, HSQC, HMBC, etc.
• Instrument is accessible to staff of the Faculty of Medicine and Hadassah Medical Center who has been trained on their use and has passed a practical and theoretical exam.

Bruker Avance III 500MHz NMR .

• Equipped with an auto-tuning multi-nuclear direct observation probe.
• Has variable temperature (20°C to 80 °C) 
• Suitable for preforming a wide range of advanced 1D and 2D proton and carbon experiments such as APT, DEPT, 1D_NOESY, 1D_TOCSY, J-RESOLVE, COSY, NOESY, TOCSY, HSQC, HMBC,T1, T2, DOSY etc
• 31P, 19F, 11B, 2H 195Pt, 15N are also configured.
• The spectrometer is running Bruker TopSpin software.
• Instruments are accessible to staff of the Faculty of Medicine and Hadassah Medical Center, who have been trained on their use and have passed a practical and theoretical exam.

A NMR service is provided on 300 MHz NMR and 500 MHz NMR both for the Faculty of Medicine staff (Internal NMR Services) as well as for external users, academic and industrial (External NMR Services).

• Internal NMR service

The Internal NMR service is available for all research at the Faculty of Medicine. Samples may be submitted for data collection only or data collection and data analysis. The NMR service includes specialist support and advice such as:

• Guidance in the choice of experiment.
• Help in setting up new experiments. 
• Quantitative and qualitative data acquisition and data analysis on the composition of a sample.
• Opportunity to undertake collaborative research projects requiring NMR expertise.

External NMR Service

The NMR lab also offers an external NMR service for outside users, both academic and industrial. A full range of NMR experiments are offered on NMR 500 MHz, including advanced one- and two-dimensional experiments. We also offer advice on experiment selection, data interpretation and reports.

For price quote please contact - Dr. Aviva Friedman-Ezra, Tel: 87030,

E-mail:    [email protected]

• People with medical implants (such as cardiac implants pacemakers, aneurysm clips, surgical clips or prostheses) should check with us before entering the NMR room.
•  All magnetic objects should be kept outside the NMR magnet room.
•  Keep electronics, credit cards and magnetic storage media outside of the NMR magnet room.

Dr. Aviva Friedman-Ezra

Faculty of Medicine ,School of Pharmacy Building

Ein Karem Campus

The Hebrew University

Tel: +972-2-6757030

Fax: +972-2-6757076

E-mail:  [email protected]

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The use of animals for scientific purposes at the University is subject to the  University Guidelines  regarding the use of animals in research and teaching. The provisions in these guidelines are based on the requirements and regulations below.

Researchers and research students intending to engage in activities involving the use of laboratory animals must:

• Obtain a permit for practice and animal experiments from the Authority for Biological and Pre-Clinical Models.   To do so, please fill out the online application form for a permit to work with laboratory animals.
• To undergo training on the ethics of using laboratory animals. See details on  trainings for researchers
• The Prevention of Cruelty to Animals Law (Experiments on Animals) - 1994  (in Hebrew)
• ​ Prevention of Cruelty to Animals - Rules - 2001 (in Hebrew)
• Authorization requirements of the AAALAC